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Plasmid#28228DepositorInsertHygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
UseRetroviralTagsEGFP and nLAP-tagExpressionMutationPromoterAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S15
Plasmid#127823PurposeProduction of recombinant human ribosomal protein S15DepositorInsertRPS15 (RPS15 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZS1[LacI-L]
Plasmid#60745PurposeContains PI driving expression dimeric LacI, the Lactose inducible repressor.DepositorInsertLacI
UseSynthetic BiologyTagsExpressionBacterialMutationwt LacI without the tetramerization domainPromoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV Lex VP16 (F442A) HA (P#1710)
Plasmid#14595DepositorInsertLexA
UseTagsHA and VP16 (F442A)ExpressionMammalianMutationThe VP16 sequence contains an F442A mutation. The…PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
DVA_DH
Plasmid#66049PurposeMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:H]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVA_EB
Plasmid#66050PurposeMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:B]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
JBEI-6822
Plasmid#84985PurposeFor expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol productionDepositorInsertMevTaco-MKco-PMKco
UseTagsExpressionBacterialMutationPromoterLacUV5Available sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL6 SMR1747
Plasmid#28029DepositorInsertMutS (mutS E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE intramol-1m
Plasmid#13551DepositorInsertFLIP-E intramol A207W (gltI E. coli)
UseTagsEYFPExpressionBacterialMutationA207W, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
HL 3722 (=HL 716 + pHL 404)
Plasmid#30084DepositorInsertpLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-trmI
Plasmid#89955PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.DepositorInserttrmI gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDTnLAPP2A frame 1
Plasmid#28227DepositorInsertHygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
UseRetroviralTagsEGFP and nLAP-tagExpressionMutationPromoterAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAL942
Plasmid#119721Purposeprecursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 CysteineDepositorInsertprecusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C
UseTagsnoneExpressionBacterialMutationAlanine 248 to Threonine, Alanine 188 to Cysteine…PromoterT7Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pKDsgRNA-xylA
Plasmid#89964PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.DepositorInsertxylA gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-lpoB
Plasmid#89959PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.DepositorInsertlpoB gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
HL 1329 (=HL 865 + pHL 344)
Plasmid#30014DepositorInsertpLtetO-1:mCherry, pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p)
Plasmid#70197PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)
Plasmid#70213Purpose1 Part of 4-part switchDepositorInsertlacI
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU3-B-RFP
Plasmid#72946PurposeLevel 3 Destination vector - Assembly of 4 Level 2 modules - ABCDDepositorInsertLevel 3 - B site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
HL 2818 (=HL 865 + pHL 489)
Plasmid#30032DepositorInsertpLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4218 (=HL 716 + pHL 1411)
Plasmid#30105DepositorInsertpLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsT7noRBS, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4212 (=HL 716 + pHL 1414)
Plasmid#30104DepositorInsertpCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsT7noRBS, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 839 (=HL 716 + pHL 108)
Plasmid#30008DepositorInsertpLlacO-1:MicC-mCherry, pLtetO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHL600
Plasmid#37563DepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 2312 (=HL 716 + pHL 787)
Plasmid#30024DepositorInsertpLtetO-1:MicC, pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3858 (=HL 716 + pHL 1304)
Plasmid#30088DepositorInsertpLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2817 (=HL 716 + pHL 720)
Plasmid#30031DepositorInsertpLtetO-1:mCherry, pLlacO-1:sodB::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4120 (=HL 716 + pHL 1370)
Plasmid#30094DepositorInsertpLtetO-1:DsrA, pCon:T7(st3) (900bps), pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2305 (=HL 716 + pHL 765)
Plasmid#30020DepositorInsertpLtetO-1:MicC, pLtetO-1:DsrA, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2308 (=HL 716 + pHL 814)
Plasmid#30022DepositorInsertpLtetO-1:MicC, pLtetO-1:OxyS, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE intramol-10u
Plasmid#13550DepositorInsertFLIP-E intramol A207R (gltI E. coli)
UseTagsEYFPExpressionBacterialMutationA207R, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
BtARR1 (1-378)
Plasmid#236264PurposeBacterial expression of bovine arrestin-1 (1-378) E379StopDepositorInsertarrestin-1 (SAG Bovine)
UseTagsExpressionBacterialMutation(1-378) E379Stop, inserted GGT codon after starti…PromoterlacAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
BtARR1 R175E
Plasmid#236263PurposeBacterial expression of bovine arrestin-1 R175E with inserted GGT codon after starting ATGDepositorInsertarrestin-1 (SAG Bovine)
UseTagsExpressionBacterialMutationR175E, inserted GGT codon after starting ATGPromoterlacAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_4_024_CrCD-marker
Plasmid#235900PurposeCytosine deaminase selection gene, codon optimized for Chlamydomonas reinhardtiiDepositorInsertCrCD-marker
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pISEre1reRNA
Plasmid#226821PurposeExpressing ISEre1 guide RNA targeting maeB site controled by PJ23119DepositorInsertISEre1 none target guide RNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
ECMAB - pET22b Ag43 160N 6H Mms6
Plasmid#225539PurposeConstruction of extracellular magnetite accumulating bacterial systems.DepositorInsertsIron binding protein
Autotransporter protein
UseTagsnoExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-Lysozyme fAb
Plasmid#214951PurposeBacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusionsDepositorInsertsVNp-anti-Lysozyme Fab heavy chain fusion
VNp-anti-Lysozyme Fab light chain fusion
UseTagsVNpExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-GFP mAb
Plasmid#214955PurposeBacterial expression of anti-GFP mAb light and heavy chain VNp fusions.DepositorInsertsVNp-anti-GFP mAb heavy chain
VNp-anti-GFP mAb light chain
UseTagsVNp6ExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS13
Plasmid#229474PurposeExpresses RssB with Q254A & N256A mutationDepositorInsertRssB (Q254A, N256A)
UseTagsExpressionBacterialMutationQ254A, N256APromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS19
Plasmid#229475PurposeExpresses RssB with E135A, E136A, & E137A mutationDepositorInsertRssB (E135A, E136A, E137A)
UseTagsExpressionBacterialMutationE135A, E136A, E137APromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF42
Plasmid#229467PurposeExpresses RssB with S29C & E229C mutationDepositorInsertRssB (S29C, E229C)
UseTagsExpressionBacterialMutationS29C, E229CPromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS21
Plasmid#229476PurposeExpresses RssB with E135A, E136A, & E137A mutation with His-tagDepositorInsertRssB (E135A, E136A, E137A)
UseTags10xHisExpressionBacterialMutationE135A, E136A, E137APromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS26
Plasmid#229471PurposeExpresses RssB with D104N mutation with His-tagDepositorInsertRssB (D104N)
UseTags10xHisExpressionBacterialMutationD104NPromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS23
Plasmid#229470PurposeExpresses RssB with D104A mutation with His-tagDepositorInsertRssB (D104A)
UseTags10xHisExpressionBacterialMutationD104APromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS18
Plasmid#229462PurposeExpresses IraD with Q68A mutation with His-tagDepositorInsertIraD (Q68A)
UseTags6xHisExpressionBacterialMutationQ68APromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS12
Plasmid#229461PurposeExpresses IraD with W64R mutation with His-tagDepositorInsertIraD (W64R)
UseTags6xHisExpressionBacterialMutationW64RPromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF40
Plasmid#229459PurposeExpresses IraD with R70A mutation with His-tagDepositorInsertIraD (R70A)
UseTags6xHisExpressionBacterialMutationR70APromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF26
Plasmid#229458PurposeExpresses IraD with Q84A mutation with His-tagDepositorInsertIraD (Q84A)
UseTags6xHisExpressionBacterialMutationQ84APromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF25
Plasmid#229457PurposeExpresses IraD with R67A mutation with His-tagDepositorInsertIraD (R67A)
UseTags6xHisExpressionBacterialMutationR67APromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only