We narrowed to 4,288 results for: PRS
-
Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only
-
pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-Cer-cMycPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSS63
Plasmid#47951PurposertTA positive feedback loop with single tet operator in the GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionExpressionYeastPromoterT3Available SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CII cat-/EE (E318Q; S431E; T432E)
Plasmid#42498DepositorInsertKaiC CII cat-/EE (E318Q; S431E; T432E)
ExpressionBacterialMutationE318Q; S431E; T432EPromotert7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CI cat- (E77Q;E78Q)
Plasmid#41886DepositorInsertKaiC CI cat -(E77Q;E78Q)
ExpressionBacterialMutationchanged glutamic acid 77 and 78 to glutaminePromotert7 promoterAvailable SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CII cat- (E318Q)
Plasmid#41887DepositorInsertKai C CII cat (E318Q)
UseBl de3 21ExpressionBacterialMutationchanged amino acid 318 E to QPromotert7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH490-tQSRTL
Plasmid#29699DepositorInserttR(CCG)J; tQ(CUG)M; tT(CGU)K; tS(CGA)C; tL(GAG)G
ExpressionYeastAvailable SinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F48
Plasmid#23072DepositorInsertyeast Histone H3-2 and Histone H4-2
ExpressionYeastMutationHistone H3 changed Lysine 14 to Glutamine, H3 K14…Available SinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F50
Plasmid#23074DepositorInsertyeast Histone H3-2 and Histone H4-2
ExpressionYeastMutationHistone H3 changed Lysine 14 to Arginine, H3 K14R…Available SinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F54
Plasmid#23078DepositorInsertyeast Histone H3-2 and Histone H4-2
ExpressionYeastMutationHistone H3 changed Lysine 9 to Glutamine, H3 K9Q …Available SinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/T22I
Plasmid#1110DepositorInsertHSP82 (HSP82 Budding Yeast)
ExpressionYeastMutationHSP82 with threonine 22 changed to isoleucine; re…Available SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G81S
Plasmid#1111DepositorInsertHSP82 (HSP82 Budding Yeast)
ExpressionYeastMutationHSP82 with glycine 81 changed to serine; regulate…Available SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G313S
Plasmid#1114DepositorInsertHSP82 (HSP82 Budding Yeast)
ExpressionYeastMutationHSP82 with glycine 313 changed to serine; regulat…Available SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/E381K
Plasmid#1115DepositorInsertHSP82 (HSP82 Budding Yeast)
ExpressionYeastMutationHSP82 with glutamic acid 381 changed to lysine; r…Available SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/A587T
Plasmid#1116DepositorInsertHSP82 (HSP82 Budding Yeast)
ExpressionYeastMutationHSP82 with alanine 587 changed to threonine; regu…Available SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/A41V
Plasmid#1117DepositorInsertHSP82 (HSP82 Budding Yeast)
ExpressionYeastMutationHSP82 with alanine 41 changed to valine; regulate…Available SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSL3 - Brr2 del113 N1104L
Plasmid#90253Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 113 amino acids and amino acid N110…AvailabilityAcademic Institutions and Nonprofits only -
pSL7 - Brr2 del247 N1104L
Plasmid#90257Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 247 amino acids and N1104LAvailabilityAcademic Institutions and Nonprofits only -
pSL11 - Brr2 del422 N1104L
Plasmid#90261Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid N110…AvailabilityAcademic Institutions and Nonprofits only -
pSL12 - Brr2 del422 Q904E
Plasmid#90262Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid Q904EAvailabilityAcademic Institutions and Nonprofits only -
pSL13 - Brr2 del422 R1107L
Plasmid#90263Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid R110…AvailabilityAcademic Institutions and Nonprofits only -
pV1338
Plasmid#111443PurposeSolo vector pV1326 + sgScADE2DepositorInsertCaCas9/sgScADE2
UseCRISPRExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pZS165 CRISPEY HH-HDV NotI
Plasmid#114451PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsExpressionYeastPromoterScGAL7pAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu5 (R)
Plasmid#98296PurposeEnhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
ExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
SigmaR1-mNeonGreen
Plasmid#226566PurposeEncodes SigmaR1 protein labeled with mNeonGreenDepositorAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAD-GamS
Plasmid#99246PurposeExpresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.DepositorInsertgamS (with C-terminal 6xHis tag)
Tags6xHis tagExpressionBacterialPromoterT7lacAvailable SinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu8
Plasmid#98297PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIS3
Plasmid#92173PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.DepositorInsertNAT1
UseGenbank: u03440.1ExpressionYeastAvailable SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
SepOTSλ
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.csr-1.Y-SUP4t
Plasmid#68060PurposegRNA for csr-1 locus in N. crassaDepositorInsertgRNA for csr-1 locus
UseCRISPR; N. crassa, fungiExpressionYeastPromoterSNR52Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-eGFP
Plasmid#32548DepositorAvailable SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEmpty_Control
Plasmid#162607PurposeEmpty control for Cas9 experiments and to clone new guide RNAs for paperDepositorInsertTef1-Cas9 with RPL25 Intron
ExpressionYeastAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNCS BFP
Plasmid#91757PurposeBacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)DepositorInsertBlue Fluorescent Protein
TagsHexa-Histidine, Xpress epitope for detection with…ExpressionBacterialMutationGFP (Y66H)PromoterT7Available SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-eGFP
Plasmid#32549DepositorAvailable SinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
ExpressionYeastPromoterTetO7Available SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human Parkin (1-465)
Plasmid#110758PurposeBacterial expression of human ParkinDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNTI661 pRPR1(TetO)-sgRNA
Plasmid#139475PurposeVector for tet-regulated sgRNA expression in yeastDepositorInsertsingle guide RNA scaffold
UseCRISPRExpressionYeastPromoterpRPR1(TetO)Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pRRL MND GFP
Plasmid#36247DepositorAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJH001
Plasmid#67641PurposeHigh copy Cas9 expression vector with LEU2 marker for yeast transformationDepositorInsertsCas9
Empty
UseCRISPRExpressionYeastAvailable SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
CuGFP-ATG8(416)
Plasmid#49423PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
TagsGFPExpressionBacterial and YeastPromoterCUP1Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNCS mTangerine
Plasmid#91763PurposeBacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)DepositorInsertmonomeric Tangerine
Tagsno tagExpressionBacterialPromoterT7Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS mHoneydew
Plasmid#91760PurposeBacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)DepositorInsertmonomeric Honeydew
Tagsno tagsExpressionBacterialPromoterT7Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS mRFP
Plasmid#91765PurposeBacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)DepositorInsertmonomeric Red Fluorescent Protein1
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAID6
Plasmid#136989PurposeYeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4DepositorInsertsdLbCpf1-VP
dSpCas9-RD1152
SaCas9
Csy4
ExpressionYeastPromoterENO2, TDH3, TEF1, and TPI1Available SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only