19,770 results

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pLenti-mCherry-Cre-blast

Plasmid

#179390

Purpose

Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

Insert

mCherry (P2A) Cre recombinase

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEF6.mCherry-TSG101

Plasmid

#38318

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

TSG101 (TSG101 Human)

Tags

mCherry

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3

Plasmid

#75127

Purpose

Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGL2-2xCLEAR-luciferase

Plasmid

#81120

Purpose

Reporter activity of the CLEAR network

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

2xCLEAR

Use

Luciferase

Tags

luciferase

Promoter

2xCLEAR

Available Since

Dec. 3, 2016

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIghg1

Plasmid

#127158

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

Depositor

Hedda Wardemann

Insert

Ighg1 (Ighg1 Mouse)

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-LC3

Plasmid

#11546

Depositor

Karla Kirkegaard

Article

Jackson et al PLoS Biol. 2005 May . 3(5):e156.

Insert

LC3B (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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MSCV-XZ066-EGFRvIII

Plasmid

#20737

Depositor

Alonzo Ross

Article

Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

Insert

EGFRvIII (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

EGFR with a 267 amino acid deletion in the extrac…

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pCLX-pTF-R1-DEST-R2-EBR65

Plasmid

#45952

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pEBG

Plasmid

#22227

Purpose

GST fusion vector

Depositor

David Baltimore

Article

Tanaka et al Mol Cell Biol. 1995 Dec . 15(12):6829-37

Type

Empty backbone

Tags

GST

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pSLQ-mgU2-47-Mango II

Plasmid

#127586

Purpose

Expresses mgU2-47-Mango II scaRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

mgU2-47 scaRNA with Mango II tag

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSBbi-pur H-2Kb

Plasmid

#111623

Purpose

Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

Depositor

Jon Yewdell

Insert

H2Kb

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Article

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Insert

Akt1 (AKT1 Human)

Tags

HA and flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pBeloBac11loxP2272

Plasmid

#60342

Purpose

This is a BAC vector that contains both wt and mutant loxP sites.

Depositor

Jon Coren

Article

Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-col4a1

Plasmid

#160317

Purpose

cfSGFP2-tagged collagen4a1 for mammalian expression

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Insert

COL4A1 (COL4A1 Human)

Tags

cfSGFP2

Expression

Mammalian

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

Plasmid

#159905

Purpose

Mutagenesis of Slc32a1

Depositor

Larry Zweifel

Insert

Slc32a1 (Slc32a1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XHA-EGFP-PEX26

Plasmid

#139054

Purpose

HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XHA-EGFP-PEX26

Use

Lentiviral

Tags

HA

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

Plasmid

#167582

Purpose

Express the ATP sensor GRAB_ATP1.0 in mammalian cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRcCMV Cep215 (Nigg CW493)

Plasmid

#41152

Depositor

Erich Nigg

Article

Graser et al J Cell Sci. 2007 Dec 15;120(Pt 24):4321-

Insert

Cep215 (CDK5RAP2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lwa_Cas13a_WT

Plasmid

#91865

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LwaCas13a

Use

CRISPR

Tags

His6-MBP-TEV site

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-VSVG

Plasmid

#11912

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Expression

Mammalian

Mutation

F64L, S65T, A206K relative the wild type GFP sequ…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA6-ARID1A

Plasmid

#39311

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV_hSynapsin_psychLight2

Plasmid

#163909

Purpose

To generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoter

Depositor

Lin Tian

Article

Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

Has Service

AAV9

Insert

psychLight2

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

Synapsin

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NOD2-EGFP

Plasmid

#131207

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Insert

NOD2 (NOD2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-Halo

Plasmid

#175296

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with HaloTag

Tags

HaloTag

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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7M8

Plasmid

#64839

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

Insert

7M8 cap

Use

AAV

Mutation

7mer insertion in the AAV2 cap

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pLDPuro-hsnSR100N

Plasmid

#35172

Depositor

Benjamin Blencowe

Article

Raj et al Mol Cell. 2011 Sep 2;43(5):843-50.

Insert

SRRM4 (SRRM4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Luc2-IRES-ZsGreen-W

Plasmid

#164432

Purpose

Lentiviral vector backbone encoding firefly luciferase and ZsGreen

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Inserts

Luciferase
ZsGreen

Use

Lentiviral

Available Since

Feb. 13, 2021

Availability

Academic Institutions and Nonprofits only

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AAV.rTH.PI.Cre.SV40

Plasmid

#107788

Purpose

AAV vector expressing Cre from rTH promoter

Depositor

James M. Wilson

Has Service

AAV Retrograde and AAV9

Insert

Cre

Use

AAV

Expression

Mammalian

Promoter

rTH

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CAGGS-EGFP

Plasmid

#22212

Purpose

AAV zinc finger donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

CAGGS-eGFP

Use

Zfn donor

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-hCRM1

Plasmid

#17647

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

CRM1 (XPO1 Human)

Tags

3x Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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19,770 results