We narrowed to 9,340 results for: UTY

1. pCRISPR2_+10D

   Plasmid

   #149391

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   10bp inserted downstream of the IHFprox site

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRISPR2_IHF_Opt

   Plasmid

   #149395

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   IHFprox site is optimized at key positions needed…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPR2_motif_scram

   Plasmid

   #149397

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Upstream motif recognized by Cas1-2/3 during spac…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMSCV-IRES-GFP-Mouse-caspase-1/11a

   Plasmid

   #183391

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Synthetic, Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K

   Plasmid

   #183357

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-4 with ICE activity (CASP4 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   ANR261ENP, D315H, M318K, T352K

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain

   Plasmid

   #183396

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domains of canine caspase-1/4 fused to catalytic domain of mouse caspase-11
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+human-Caspase-4-catalytic domain

   Plasmid

   #183395

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domains of canine caspase-1/4 fused to catalytic domain of human caspase-4
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSCV-IRES-GFP-Mouse-caspase-1/11b

   Plasmid

   #183392

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domain of mouse caspase-1 fused to mouse caspase-11 (Casp1 Synthetic, Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEF1α-woolly-monkey-retroCHMP3(148)-HA

   Plasmid

   #154250

   Purpose

   expresses prematurely truncated woolly monkey retroCHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C-terminal truncation of retroCHMP3 at amino acid…

   Promoter

   EF1-alpha

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF1α-pygmy-marmoset-retroCHMP3(148)-HA

    Plasmid

    #154251

    Purpose

    expresses prematurely truncated pygmy marmoset retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal truncation of retroCHMP3 at amino acid…

    Promoter

    EF1-alpha

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGB-Co-NF-kB

    Plasmid

    #180206

    Purpose

    Expression GAL4-fusion of Co-NF-kB in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kB
    

    Use

    Tags

    GAL4 (1-147) fusion

    Expression

    Yeast

    Mutation

    aa 2-1224

    Promoter

    AdhI

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. gMUM2-Citrine

    Plasmid

    #181965

    Purpose

    MUM2 protein fused to Citrine, under control of native promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    MUM2 (MUM2 Mustard Weed)
    

    Use

    Tags

    Citrine YFP

    Expression

    Plant

    Mutation

    Promoter

    MUM2

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MUM2SP-Citrine

    Plasmid

    #181963

    Purpose

    MUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    MUM2 signal peptide, fused to the Citrine YFP in pAD
    

    Use

    Tags

    Citrine YFP

    Expression

    Plant

    Mutation

    Promoter

    MUM4pro 1.5kb fragment

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCm N-term MBP SMT3 NikJC199U

    Plasmid

    #174363

    Purpose

    Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Use

    Tags

    8xHis, MBP, SMT3, and TEV

    Expression

    Bacterial

    Mutation

    C199U

    Promoter

    T7

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPPC018.AAV

    Plasmid

    #171146

    Purpose

    Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    hAAVS1 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPPC030.306

    Plasmid

    #171151

    Purpose

    Mevalonate production pathway with J306 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J306 scRNA
    J3-BBa_J23117-mvaES
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J3-BBa_J23117

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMP71-mGFP-LLO-hPMEL

    Plasmid

    #174607

    Purpose

    expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitope

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    expresses palmitoylated GFP w/C-term fusion of the LLO190 epitope and human hPMEL epitope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-kcnj12a

    Plasmid

    #173141

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj12a (kcnj12a Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-D-kcnj16

    Plasmid

    #173146

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj16a (kcnj16 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Stop codon removed

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221-kcnj19b

    Plasmid

    #173149

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj19b (kcnj19b Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR-D-kcnj1a.1

    Plasmid

    #173125

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj1a (kcnj1a.1 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR221-kcnj11l

    Plasmid

    #173140

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj11l (kcnj11l Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-anticalin (E60amb)-His

    Plasmid

    #168043

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 60 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-anticalin (E143amb)-His

    Plasmid

    #168046

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 143 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-anticalin (S87amb)-His

    Plasmid

    #168044

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ser 87 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-anticalin (N116amb)-His

    Plasmid

    #168045

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 116 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-anticalin (N21amb)-His

    Plasmid

    #168041

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 21 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-Fgβ-anticalin-His-ybbr

    Plasmid

    #168040

    Purpose

    Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag, Fgβ peptide, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-anticalin (I55amb)-His

    Plasmid

    #168042

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ile 55 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-CVm-Sec22b

    Plasmid

    #168029

    Purpose

    Expresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-Sec22b(A131-H190)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-CVm-VAMP8

    Plasmid

    #168028

    Purpose

    Expresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP8(G7-R67)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG

    Plasmid

    #139964

    Purpose

    Over-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-5U6-sgRNAs-hsyn-EGFP

    Plasmid

    #112213

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    sgRNAs
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMIII-Neo-mChe

    Plasmid

    #153423

    Purpose

    Retroviral (MSCV) expression of mCherry fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGI3EM11

    Plasmid

    #135483

    Purpose

    Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunH2Bpr hph-GFP ADH1t
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2Bpr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGI3EM29

    Plasmid

    #135489

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mCitrine
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGI3EM30

    Plasmid

    #135490

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mClover3

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mClover3
    

    Use

    Tags

    mClover3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGI3EM31

    Plasmid

    #135491

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mCerulean3
    

    Use

    Tags

    mCerulean3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHCKan-yibDp-CBD-hGLY

    Plasmid

    #134596

    Purpose

    Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    human glycine acyltransferase 2 (GLYATL2 )
    

    Use

    Tags

    chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. myc-Miro2 (lacking TM)

    Plasmid

    #127614

    Purpose

    Human Miro2 with the transmembrane deleted

    Depositor

    Josef Kittler

    Article

    Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

    Insert

    Miro2 (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Transmembrane deletion

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET20b-YbAnbu

    Plasmid

    #129637

    Purpose

    Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbAnbu
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    V14A and G107H

    Promoter

    T7

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET20b-YbAnbuT1A

    Plasmid

    #129638

    Purpose

    Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbAnbuT1A
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    T1A, V14A and G107H

    Promoter

    T7

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k

    Plasmid

    #127403

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon, W340A,…

    Promoter

    CMV

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k

    Plasmid

    #127407

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, K305H,…

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k

    Plasmid

    #127401

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon, W340A,…

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMX-eSOX2NRR

    Plasmid

    #118825

    Purpose

    Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSOX2NRR (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    NRR: E46N, I53R, K57R

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCDB367

    Plasmid

    #110277

    Purpose

    gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

    Depositor

    Christopher Bahl

    Article

    Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

    Insert

    gHEEE_02
    

    Use

    Tags

    10-His, Tev recognition peptide, and mature DsbC …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Hy_pMT dati MCS Exon

    Plasmid

    #110119

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    dati (dati Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Hy_pMT dati Exons 1-3

    Plasmid

    #110113

    Purpose

    Drosophila dati minigene plasmid that expresses both a linear RNA and a circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    dati (dati Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET28b+R3(R112C/T180C)C

    Plasmid

    #108194

    Purpose

    Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C)-P450cam fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge