166,224 results

1. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor MCS Rosa26

   Plasmid

   #37200

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

   Inserts

   Rosa26 left targeting arm
   MCS
   Rosa26 right targeting arm
   

   Use

   Genomic targeting - zinc finger

   Available Since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rich-pT7

   Plasmid

   #108445

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Promoter

   pT7

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti.PGK.blast-Renilla_Luciferase

   Plasmid

   #74444

   Purpose

   Control lentiviral vector expressing Renilla luciferase

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Renilla Luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB-cLUC

   Plasmid

   #174051

   Purpose

   Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

   Depositor

   Alberto Macho

   Article

   Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Plant

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-DNAJC13(FL)

   Plasmid

   #246660

   Purpose

   Mammalian expression of full length, eGFP tagged DNAJC13

   Depositor

   Braden Lobingier

   Article

   Adoff et al Mol Biol Cell. 2025 Sep 1;36(9):ar114. d

   Insert

   DNAJC13 (DNAJC13 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC1-SypHer3s

   Plasmid

   #108118

   Purpose

   Ratiometric pH probe with enhanced brightness for mammalian cells expression.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s
   

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Red Glifon 300

   Plasmid

   #163115

   Purpose

   Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

   Depositor

   Takashi Tsuboi

   Article

   Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

   Insert

   Red Glifon 300
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGADT7-GW

   Plasmid

   #61702

   Purpose

   Gateway compatible Y2H vector - AD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL004

    Plasmid

    #215336

    Purpose

    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)

    Depositor

    Gene-Wei Li

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    hyper-pSpank

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TfR-pHuji

    Plasmid

    #61505

    Purpose

    Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Tags

    pHuji

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. cyto-iATPSnFR1.0

    Plasmid

    #102550

    Purpose

    cytoplasmic ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Expression

    Mammalian

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPCX2-MT1-MMP-eGFP

    Plasmid

    #89819

    Purpose

    Express in MT1-MMP mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    MT1-MMP (MMP14 Human)
    

    Use

    Retroviral

    Tags

    eGFP

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHiUGE TurboID-HA donor (all-in-one) ORF0

    Plasmid

    #200383

    Purpose

    Tagging endogenously expressed proteins with TurboID-HA, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTU2-AmyE-Pveg-eGFP

    Plasmid

    #112792

    Purpose

    Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    Pveg-eGFP
    

    Expression

    Bacterial

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFR_HCV_xb

    Plasmid

    #11510

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. TGFB1_pLX307

    Plasmid

    #98377

    Purpose

    Lentiviral expression of TGFB1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    TGFB1 (TGFB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Src-Y530F

    Plasmid

    #124659

    Purpose

    Human c-Src with Y530F mutation

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    cSrc (SRC Human)
    

    Expression

    Mammalian

    Mutation

    Y530F

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTetON-EGFP

    Plasmid

    #89453

    Purpose

    Tet/Dox-inducible expression of EGFP in mammalian cells (control)

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234446

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Promoter

    GFAP

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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