4,073 results

1. pLV-tTRKRAB-red

   Plasmid

   #12250

   Depositor

   Didier Trono

   Article

   Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

   Insert

   TetR-KRAB fusion and dsRED
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   dsRed added to backbone.

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PKC alpha DN

   Plasmid

   #21235

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC alpha (PRKCA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K368R

   Promoter

   Available since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMKO.1 puro GFP shRNA

   Plasmid

   #10675

   Purpose

   Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

   Depositor

   William Hahn

   Article

   Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

   Insert

   GFP shRNA
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PT3.5/CMV-EGFRvIII

   Plasmid

   #20280

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   EGFRvII (EGFR Human)
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   See depositor comments below.

   Promoter

   Available since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGFP-HBx

   Plasmid

   #24931

   Depositor

   Xin Wang

   Article

   Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

   Insert

   HBx
   

   Use

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

   Plasmid

   #50458

   Purpose

   Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV8

   Insert

   rM3D-mCherry
   

   Use

   AAV

   Tags

   HA and mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. WT NFAT2

   Plasmid

   #11101

   Depositor

   Anjana Rao

   Article

   Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

   Insert

   NFAT2 (Nfatc1 Mouse)
   

   Use

   Retroviral

   Tags

   3xHA and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pABCb10-GFP

   Plasmid

   #19031

   Depositor

   Orian Shirihai

   Article

   Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

   Insert

   ATP-binding cassette, sub-family B (MDR/TAP), member 10 (Abcb10 Mouse)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR/pTER+ (430-1)

   Plasmid

   #17453

   Purpose

   Gateway entry vector for shRNA expression under H1/TO promoter

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   RNAi; Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-gw

    Plasmid

    #18656

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    Gateway cassette A
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2-NXE+mem-KillerRed

    Plasmid

    #45761

    Depositor

    Michael Levin

    Insert

    Membrane-bound KillerRed
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    CMV IE94

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7-EGFP-C1-HsDCP1a

    Plasmid

    #25030

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    DCP1a (DCP1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc-Neo-OVA

    Plasmid

    #22533

    Depositor

    Michael Bevan

    Article

    Moore et al Cell. 1988 Sep 9. 54(6):777-85.

    Insert

    OVA (OVAL Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. N-Myc-53BP1 WT pLPC-Puro

    Plasmid

    #19836

    Depositor

    Titia de Lange

    Article

    Dimitrova et al Nature. 2008 Oct 19. ():.

    Insert

    p53 Binding Protein 1 (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mTLR4 flag

    Plasmid

    #13087

    Depositor

    Ruslan Medzhitov

    Insert

    TLR4 (Tlr4 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-19 have been replaced with mammalia…

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBiFC-bJunVN155(I152L)

    Plasmid

    #27098

    Depositor

    Chang-Deng Hu

    Article

    Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

    Insert

    bJunVN155(I152L) (Jun Rat, and Aequorea Victoria)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    I152L in Venus(1-154)

    Promoter

    Available since

    Jan. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGH8 - pRAB-3::mCherry::unc-54utr

    Plasmid

    #19359

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Prab-3::mCherry
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C1 NFAT3

    Plasmid

    #10961

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    NFAT3 (NFATC4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pdCas9-DNMT3A-PuroR_v2

    Plasmid

    #74407

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 GFP LIC cloning vector (6D)

    Plasmid

    #30127

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pnos-Cas9-nos

    Plasmid

    #62208

    Purpose

    Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    nanos

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SIRT4 Flag

    Plasmid

    #13815

    Purpose

    Mammalian expression of human SIRT4 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT4 (SIRT4 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

    Plasmid

    #24334

    Depositor

    Didier Stainier

    Article

    Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

    Insert

    H2B
    

    Use

    Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Mutation

    mCherry driven by an HSP70 promoterCerulean drive…

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMSCVhygro-Cre

    Plasmid

    #34565

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    March 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Raptor_2 shRNA

    Plasmid

    #1858

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

    Plasmid

    #29722

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pWPXLd

    Plasmid

    #12258

    Depositor

    Didier Trono

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRK7-HA-S6K1-KR

    Plasmid

    #8985

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K100R, kinase-inactive

    Promoter

    Available since

    Jan. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-SADB19N

    Plasmid

    #32630

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    B19N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

    Plasmid

    #59791

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized VP64 dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EGFPC1-hMyoX

    Plasmid

    #47608

    Depositor

    Emanuel Strehler

    Article

    Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

    Insert

    MyoX (MYO10 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. WT NFAT1

    Plasmid

    #11100

    Depositor

    Anjana Rao

    Article

    Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p4947 pcDNA4c hBrd4 full-length

    Plasmid

    #14441

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (BRD4 Human)
    

    Use

    Tags

    6xHis, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Flag pLJM1 RagB 99L

    Plasmid

    #19315

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding B (RRAGB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q99L

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET23b-Casp9-His

    Plasmid

    #11829

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

    Insert

    Caspase 9 (CASP9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

36. pBabe EGFR(L858R/T790M)

    Plasmid

    #32073

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L858R/T790M

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1/opto-a1AR-EYFP

    Plasmid

    #20947

    Purpose

    Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Mar 18. ():.

    Insert

    opto-a1AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pARF6-CFP

    Plasmid

    #11382

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p3TP-lux

    Plasmid

    #11767

    Depositor

    Jeff Wrana, Joan Massague

    Article

    Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

    Insert

    3TP
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pBabe EGFR Del1

    Plasmid

    #32062

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Del1(E746-A750)

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge