161,592 results

1. pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

   Plasmid

   #126679

   Purpose

   Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

   Depositor

   Hee Won Yang

   Article

   Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

   Insert

   Rb (a.a 886-928)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNeae2

   Plasmid

   #168300

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

   Insert

   Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
   

   Use

   Tags

   6xHis, E-tag, and myc-tag

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-G-Flamp2

   Plasmid

   #192782

   Purpose

   green biosensor for cAMP in mammalian cells

   Depositor

   Jun Chu

   Article

   Liu et al Front Pharmacol. 2022 May 12;13:902290.

   Insert

   G-Flamp2
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AiP12157 - pAAV-AiE0395h-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2157)

   Plasmid

   #208146

   Purpose

   Direct-expressing SYFP2 in oligodendrocytes AAV Virus

   Depositor

   The Allen Institute for Brain Science, Boaz Levi

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3-EF1a-YapS127A

   Plasmid

   #46049

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S127A

   Promoter

   EF1a

   Available since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

6. DLX2-hygro

   Plasmid

   #97330

   Purpose

   Generating lentiviruses to express Dlx2 and hygromycin resistance

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

   Insert

   DLX2 (Dlx2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TetO

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCmini-iCAP-AAV.CAP-B10

   Plasmid

   #175004

   Purpose

   non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

   Depositor

   Viviana Gradinaru

   Article

   Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

   Insert

   Synthetic construct isolate AAV.CAP-B10 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   7 amino acid substitution between VP1 452 and VP1…

   Promoter

   p41

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA-FLAG-METTL3-APPA

   Plasmid

   #160251

   Purpose

   Express catalytic inactive FLAG-METTL3 in human cells

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changes in aa 395–398, DPPW → APPA

   Promoter

   CMV

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MCP-mCherry

   Plasmid

   #207668

   Purpose

   To express fluorescently labeled MS2 coat binding protein (MCP)

   Depositor

   Christine Mayr

   Article

   Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

   Insert

   MCP-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIIS948-CXCR4

    Plasmid

    #238213

    Purpose

    TasR tigRNA expression to target CXCR4 under U6 promoter in human cells.

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    tigRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-MBP-mSA2

    Plasmid

    #52319

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. V5-TurboID-NES_pCDNA3

    Plasmid

    #107169

    Purpose

    expresses V5-tagged TurboID in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid

    #26976

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV5, AAV8, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. phU6-gRNA

    Plasmid

    #53188

    Purpose

    Expresses the S. pyogenes sgRNA from the human U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Delta(Pro-MP)ADAM10-HA

    Plasmid

    #65107

    Purpose

    Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    Delta(Pro-MP)ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted AA 19-455

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA-miRFP670nano3

    Plasmid

    #184664

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-PE2

    Plasmid

    #132775

    Purpose

    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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