166,169 results

1. mRuby-Clathrin-15

   Plasmid

   #55852

   Purpose

   Localization: Clathrin Vesicles, Excitation: 558, Emission: 605

   Depositor

   Michael Davidson

   Insert

   Clathrin (CLTB Human)
   

   Tags

   mRuby

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pD649-HAsp-PILRA-COMP5AP-AviTag-9xHis

   Plasmid

   #157142

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PILRA (PILRA Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWPI-LUC7L-3xFLAG

   Plasmid

   #201640

   Purpose

   Over-expression of 3xFLAG-tagged human LUC7L

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   LUC7L (LUC7L Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pF hPGK mc2 IRES hygro

   Plasmid

   #206238

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pf CAG mc2 IRES blast

   Plasmid

   #206235

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pf CAG mc2 IRES puro

   Plasmid

   #206233

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. NPC1-CFP P692S

   Plasmid

   #58457

   Purpose

   expression of NPC1-CFP sterol sensing domain mutant

   Depositor

   Matthew Scott

   Article

   Ko et al Mol Biol Cell. 2001 Mar;12(3):601-14.

   Insert

   NPC1 (Npc1 Mouse)
   

   Tags

   ECFP and His6

   Expression

   Mammalian

   Mutation

   P692S sterol sensing domain mutant

   Promoter

   CMV

   Available Since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pT7-EGFP-C1-HsPAN3

   Plasmid

   #37374

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsPAN3 (PAN3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. MERTK gRNA (BRDN0001145505)

   Plasmid

   #76444

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MERTK

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MERTK (MERTK Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEmerald-CD151-7

    Plasmid

    #54032

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CD151 (CD151 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pD649-HAsp-FLT1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156962

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    FLT1 (FLT1 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF1722

    Plasmid

    #144305

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    DMRT3 (DMRT3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-NQL005-SOX2-sgRNA

    Plasmid

    #175553

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKH161_FLAG-APEX2-YIPF3 WT

    Plasmid

    #214980

    Purpose

    Flag-APEX2 tagged YIPF3

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-APEX2-YIPF3 (YIPF3 Human)
    

    Use

    Lentiviral

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221_SLC22A3

    Plasmid

    #132024

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A3 (SLC22A3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pHH0103_YWHAE_14-3-3

    Plasmid

    #109942

    Purpose

    Protein expression and purification of YWHAE_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAE_14-3-3
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHygRR2

    Plasmid

    #200075

    Purpose

    hygR selection mScarlet 2X marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Expression

    Worm

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-MBP-TEVpCS-ADGRL4 CTF-FLAG

    Plasmid

    #217709

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL4 (ADGRL4 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-406

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ADRB2-DuET

    Plasmid

    #213181

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADRB2 (ADRB2 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-MBP-TEVpCS-ADGRL3 CTF-FLAG

    Plasmid

    #217708

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL3 (ADGRL3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-841

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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