We narrowed to 9,389 results for: Coli

1. pLG025 (DUF4297 + HerA)

   Plasmid

   #157903

   Purpose

   Expresses the DUF4297 + HerA defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DUF4297 + HerA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEutRwc

   Plasmid

   #216334

   Purpose

   An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

   Depositor

   Mark Styczynski

   Article

   McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

   Inserts

   EutR
   Superfolder green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. ykgMO-IGS-LuxCDABE

   Plasmid

   #207232

   Purpose

   Calprotectin sensing promoter with luciferase reporter

   Depositor

   Arthur Prindle

   Article

   Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   ykgMO-IGS
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNVLTv2-pvdIJD-Prha-base

   Plasmid

   #169244

   Purpose

   Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   rhaRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhaS

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSF279

   Plasmid

   #167674

   Purpose

   Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pP3-GFP

   Plasmid

   #53436

   Purpose

   Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   P3 promoter
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   P3

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLG032 (ppl)

   Plasmid

   #157910

   Purpose

   Expresses the ppl defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   ppl (PHP-ATPase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLG028 (hhe)

   Plasmid

   #157906

   Purpose

   Expresses the hhe defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   hhe (DUF4011-helicase-Vsr-DUF3320)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBbS8c-ddcpf1-Δ

   Plasmid

   #153039

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E993A

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDR111_mannose_Bgal no NLS

    Plasmid

    #188399

    Purpose

    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    Promoter

    D-mannose

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAJM.690

    Plasmid

    #108533

    Purpose

    PcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PcaUAM + P3B5B-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PcaU: K14N A165T N201H

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLG022 (dsr1)

    Plasmid

    #157900

    Purpose

    Expresses the dsr1 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    dsr1 (SIR2-DUF4020)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTH847-gst-optsc

    Plasmid

    #96908

    Purpose

    Plasmid for yeast expression of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    yeast codon optimised glutathione-S transferase
    

    Use

    Tags

    8xhistidine

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 4A3.PBAD-repL(AT2).J23115-gfp

    Plasmid

    #197873

    Purpose

    Arabinose induced expression of repL(AT2) gene, which triggers DNA replication in trans, weak level of constitutive GFP expression

    Depositor

    Baojun Wang

    Article

    Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

    Insert

    part kilA/repL(AT2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBbA5c-MevT-MBI (JBEI-3085)

    Plasmid

    #87950

    Purpose

    the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    MevT-MBI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAJM.884

    Plasmid

    #108536

    Purpose

    AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    AcuRAM + PAcu-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AcuR: E80K R154H L202M

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLG016 (ApeA)

    Plasmid

    #157894

    Purpose

    Expresses the ApeA defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    ApeA (putative HEPN RNase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRU325

    Plasmid

    #167676

    Purpose

    Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRT802

    Plasmid

    #68113

    Purpose

    Integration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.

    Depositor

    Maggie Smith

    Article

    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

    Inserts

    aac3(IV)
    aph11
    oriT
    attP
    int
    

    Use

    Streptomyces integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-mCherryGGBsMTS

    Plasmid

    #133625

    Purpose

    Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherryGGBsMTS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. DHFR-myc

    Plasmid

    #20214

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    DHFR (folA E. coli)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT2SK

    Plasmid

    #59383

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-kan
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRecE564T

    Plasmid

    #226825

    Purpose

    Expressing RecE*T controled by Ptrc promoter

    Depositor

    Sheng Yang

    Article

    Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

    Insert

    recE*T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAJM.683

    Plasmid

    #108531

    Purpose

    BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    BetIAM + PBetI-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BetI: S115I

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT2SC

    Plasmid

    #59382

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a T7pCONS sfGFP

    Plasmid

    #154462

    Purpose

    pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTU1-A-RFP

    Plasmid

    #72939

    Purpose

    Level 1 Destination vector - Position A

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 1 - A site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Beta-lactamase - G717C (nucleotide) - silent muta…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKL002

    Plasmid

    #98921

    Purpose

    pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Inserts

    PROPS
    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

    Plasmid

    #110554

    Purpose

    miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    araC-ParaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AraC-ParaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJM251 (miniCTX1-araC-ParaBAD)

    Plasmid

    #110567

    Purpose

    Integration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    araC-ParaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araC-ParaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTKhc-yibDp-GFP-cp6

    Plasmid

    #134938

    Purpose

    Low phosphate inducible circular permutation of GFP

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    GFPcp6
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKB083

    Plasmid

    #181952

    Purpose

    IPTG-inducible expression of PhoQ

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    PhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PhoQ-Ptac; lacI-PlacIq

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ABE8r-R153del

    Plasmid

    #208296

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-R153del-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-LplA(W37V) Coumarin PRIME ligase

    Plasmid

    #25845

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA-W37V
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    W37V

    Promoter

    Available since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSET FLII12Pglu-700uDelta6

    Plasmid

    #13568

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLIPglu F16A Y12S-ECFP-D13R, 6aa linker
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Feb. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. prCAG-BoxB

    Plasmid

    #191122

    Purpose

    Transcription of 47xCAG and 12xBoxB repeats from a tetracycline-inducible promoter

    Depositor

    Ariel Lindner

    Article

    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

    Inserts

    rCAG
    BoxB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tetracycline-inducible promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAJM.969

    Plasmid

    #108537

    Purpose

    MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    MphRAM + EryR + PMph-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MphR: N94D

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pXint129

    Plasmid

    #178208

    Purpose

    Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

    Depositor

    Gregory Phillips

    Article

    Platt et al Plasmid. 2000 Jan . 43(1):12-23.

    Insert

    xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pST5832

    Plasmid

    #36256

    Depositor

    Carolyn Bertozzi, Jessica Seeliger

    Article

    Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

    Insert

    lacZ
    

    Use

    Synthetic Biology; Mycobacteria replicating vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Modified Hsp60

    Available since

    Nov. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge