164,577 results

1. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

   Plasmid

   #60904

   Purpose

   The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA24N-ligase

   Plasmid

   #87741

   Purpose

   IPTG-inducible expression of T4 DNA ligase for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-AAVS1-5-1kbdonor

   Plasmid

   #234735

   Purpose

   PseCAST crRNA targeting AAVS1-5, with 1 kb donor transposon

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   AAVS1-5 crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti sgRNA(MS2)_puro backbone

   Plasmid

   #73795

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AbVec1.1-IGKC

   Plasmid

   #80796

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-TPNOX

   Plasmid

   #87853

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   TPNOX
   

   Use

   Tags

   C-terminal FLAG tag with a linker

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TETv4

   Plasmid

   #167983

   Purpose

   Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   TETv4 (TET1CD-XTEN80-dCas9)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   3xNLS, TET1 catalytic domain, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-ERT2-iCre-ERT2

   Plasmid

   #178059

   Purpose

   Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ERT2-iCre-ERT2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human synapsin 1 (Syn) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-15b-EK_C122S_His5

   Plasmid

   #49048

   Purpose

   Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

   Depositor

   Hans Brandstetter

   Article

   Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

   Insert

   Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
   

   Use

   Tags

   5xHis

   Expression

   Bacterial

   Mutation

   P92R, E185D, C122S

   Promoter

   T7

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCXLE-hOCT3/4-shp53-F

    Plasmid

    #27077

    Purpose

    Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRLSIN.cPPT.PGK-GFP.WPRE

    Plasmid

    #12252

    Purpose

    3rd generation lentiviral backbone

    Depositor

    Didier Trono

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. V5-LOV-Turbo-NES

    Plasmid

    #199545

    Purpose

    expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NES and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3 Promoter Vector

    Plasmid

    #212939

    Purpose

    Control vector with constitutive firefly luciferase expression

    Depositor

    Debrya Groskreutz

    Insert

    luc+
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    WT

    Promoter

    SV40

    Available Since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pUC57-mitoTPNOX

    Plasmid

    #87854

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    mitoTPNOX
    

    Use

    Tags

    C-terminal FLAG tag with a linker and mitochondri…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. NG-ABE8e

    Plasmid

    #138491

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9 (NG)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGM1190

    Plasmid

    #69994

    Purpose

    OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCL1271

    Plasmid

    #217963

    Purpose

    Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.

    Depositor

    Andrew Bowman

    Article

    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

    Insert

    Imp5 (IPO5 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

    Plasmid

    #138610

    Purpose

    Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Inserts

    Hyperactive AID
    T7 RNA Polymerase
    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+_hsTRPA1_myc_dTom

    Plasmid

    #183179

    Purpose

    myc tagged hsTRPA1 used for cell transfection

    Depositor

    Sreekanth Chalasani

    Article

    Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

    Inserts

    hsTRPA1 (TRPA1 Human)
    dTom
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-LgBit

    Plasmid

    #234738

    Purpose

    Mammalian expression of large NanoBit fragment (LgBit)

    Depositor

    Nevin Lambert

    Article

    Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

    Insert

    LgBit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge