8,964 results
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Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
ExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 beta2
Plasmid#20355DepositorAvailable SinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSGV-1D3-28Z All ITAMs intact
Plasmid#107226PurposeAnti-mouse CD19 CAR with CD3 ITAMs intactDepositorInsert1D3-28Z All ITAMs intact
UseRetroviralTagsNoneMutationNoneAvailable SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-NLRP3
Plasmid#75127PurposeExpresses N-terminal Flag-tagged NLRP3 in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
TagsFlagExpressionMammalianPromoterCMVAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry
Plasmid#64324PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianPromoterCBh and U6Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBB542
Plasmid#27395DepositorInsertdnaK, dnaJ, groESL
ExpressionBacterialAvailable SinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-KRAB
Plasmid#50917PurposeTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterTREAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_puro optimized backbone
Plasmid#73797Purposeoptimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.TurboRFP.WPRE.RBG
Plasmid#105548PurposeAAV expression of TurboRFP from CMV promoterDepositorHas ServiceAAV1 and AAV8InsertTurboRFP
UseAAVExpressionMammalianPromoterCMVAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
myc-Rictor corrected
Plasmid#11367PurposeMammalian expression of myc tagged human RICTORDepositorAvailable SinceApril 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Myc-His beta4
Plasmid#16039DepositorAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
HDAC8 Flag
Plasmid#13825DepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
GAL4UAS-Luciferase reporter
Plasmid#64125PurposePhotoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.DepositorInsertGAL4UAS
UseLuciferaseTagsluciferaseExpressionMammalianPromoterGAL4UASAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFD1-dU6:1gRNA
Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRExpressionInsectPromoterdU6-1Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GqDREADD-dTomato-Fishell-4
Plasmid#83897PurposeGq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InserthM3Dq
UseAAVExpressionMammalianPromoterhDlxAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-beta2-adrenergic receptor
Plasmid#66994PurposeBeta-2-adrenergic receptor tagged with HaloTag on amino terminusDepositorInsertHaloTag-beta2-adrenergic receptor (ADRB2 Human)
TagsHuman Growth hormone Signal Sequence-HaloTagExpressionMammalianMutationThe signal sequence from human growth hormone was…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 1R TALEN
Plasmid#35432PurposeTALEN designed to target the human AAVS1 locusDepositorInserthAAVS1 1R TALEN
ExpressionMammalianAvailable SinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-PKM2
Plasmid#64698PurposeExpression of GFP tagged PKM2 in mammalian cellsDepositorAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoLbNOX
Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
TagsFlag tag with linker and mitochondrial targeting …MutationHuman codon optimizedAvailable SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-19b_MMLV-RT
Plasmid#153312PurposeBacterial expression of MMLV reverse transcriptaseDepositorInsertMMLV Reverse Transcriptase
Tags10x HisExpressionBacterialAvailable SinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-shRNA-ctrl
Plasmid#85741PurposeshRNA ctrlDepositorInsertshRNA ctrl
UseAAVTagsEYFPAvailable SinceApril 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG415GAL-ccdB
Plasmid#14145DepositorTypeEmpty backboneUseGateway CloningExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
5HRE/GFP
Plasmid#46926Purposehypoxia-responsive enhanced green fluorescent protein (EGFP)-based systemDepositorInsert5X HRE of VEGF (VEGFA Human)
Tagsd2EGFPExpressionMammalianMutationfive copies of a 35-bp fragment from the hypoxia-…PromoterEF-1α promoterAvailable SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
tau/pET29b
Plasmid#16316DepositorInserttau (MAPT Human)
ExpressionBacterialAvailable SinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
SUPERBLADE5
Plasmid#134913PurposeEmpty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyPromoterU6Available SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro-ARID1A
Plasmid#39478PurposeTetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vectorDepositorAvailable SinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC013 - Twinstrep-SUMO-huLwCas13a
Plasmid#90097PurposeTwinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.DepositorInsertLwCas13a
UseCRISPRTags6xHis-Twin Strep-SUMOExpressionBacterialAvailable SinceJune 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL4-ERSE2-luc2P-Hygro
Plasmid#101790PurposeLuciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)DepositorInsert3xERSE-II
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP GFP-NBR1
Plasmid#38283DepositorAvailable SinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA AXL
Plasmid#105932PurposeMammalian expression of myc-His tagged AXLDepositorAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only