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Showing: 4401 - 4450 of 13557 results

  28. pCas3-CDA1

    Plasmid
    #229534
    Purpose
    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229534-plasmid-map-sequence-id-458891

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  36. pUC-hU6-crBACH1-array_EF1a-BFP

    Plasmid
    #224787
    Purpose
    BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    crBACH1-1, crBACH1-2, crBACH1-3,
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and hU6-2xTetO
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224787-plasmid-map-sequence-id-450827

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  37. pUt-mNF-Cas13d

    Plasmid
    #224789
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224789-plasmid-map-sequence-id-445954

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  39. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

    Plasmid
    #169135
    Purpose
    E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Coh7
    Use
    Tags
    3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169135-plasmid-map-sequence-id-361163

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  45. pC3.1_CMV_oROS-G_LF(C199S)

    Plasmid
    #216112
    Purpose
    Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.01.31.578117
    Insert
    oROS-G_LF(C199S)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216112-plasmid-map-sequence-id-434850

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  47. AAV2_CAG_oROS-G_LF(C199S)

    Plasmid
    #216116
    Purpose
    Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.01.31.578117
    Insert
    oROS-G_LF(C199S)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216116-plasmid-map-sequence-id-429714

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  48. pCRISPR-cBEST-v2-SF14P

    Plasmid
    #209448
    Purpose
    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter
    Depositor
    Tilmann Weber
    Article
    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021
    Insert
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PtipA; sgRNA expression from SF14P promoter
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209448-plasmid-map-sequence-id-416625

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  49. pCRISPR-cBEST-v2-kasOP*

    Plasmid
    #209447
    Purpose
    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter
    Depositor
    Tilmann Weber
    Article
    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021
    Insert
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PtipA; sgRNA expression from kasOP* promoter
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209447-plasmid-map-sequence-id-416622

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Showing: 4401 - 4450 of 13557 results