8,866 results

1. COM-epegRNA-Dnmt1

   Plasmid

   #211376

   Purpose

   Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   COM-epegRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

   Plasmid

   #31482

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (Sce Target) EF Puro
   

   Use

   Lentiviral

   Mutation

   mCherry has M9S and M16L mutations. This is what …

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF3549

   Plasmid

   #145025

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. circRNA-synIRES-R25-NanoLuc

   Plasmid

   #188116

   Purpose

   Template plasmid to transcribe circular RNA encoding NanoLuc

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   NanoLuc
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-GFP11-Clathrin light chain

   Plasmid

   #70217

   Purpose

   Expresses GFP11-clathrin light chain in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP11-Clathrin light chain (CLTA Human)
   

   Tags

   GFP11

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry

   Plasmid

   #176016

   Purpose

   Mammalian expression of mCherry. Parton lab clone DFQ

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   mCherry
   

   Expression

   Mammalian

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas

   Plasmid

   #62225

   Purpose

   Constitutive expression of cas9 and inducible expression of lambda RED and sgR

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Has Service

   Cloning Grade DNA

   Insert

   cas9
   

   Use

   CRISPR; Lambda red recombinase

   Expression

   Bacterial

   Promoter

   native cas9 promoter

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Plasmid

   #179105

   Purpose

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+HyPer7

   Plasmid

   #136466

   Purpose

   Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLCNICK

    Plasmid

    #84653

    Purpose

    Genome editing for Lactobacillus casei Lc2W

    Depositor

    Sheng Yang

    Article

    Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

    Inserts

    Cas9 nickase
    sgRNA
    homology arms of LC2W_2179
    

    Use

    CRISPR

    Mutation

    D10A

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFR-GFP

    Plasmid

    #32751

    Purpose

    Mammalian expression of epidermal growth factor receptor (EGFR) fused to EGFP

    Depositor

    Alexander Sorkin

    Article

    Carter et al J Biol Chem. 1998 Dec 25;273(52):35000-7

    Insert

    EGFR (EGFR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-Hygro

    Plasmid

    #127763

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-NT*-HRV3CP

    Plasmid

    #162795

    Purpose

    Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

    Depositor

    Gottfried Otting

    Article

    Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

    Insert

    Human rhinovirus 14 3C protease
    

    Tags

    NT* solubility tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Oct4-IRES-eGFP-PGK-Neo

    Plasmid

    #48681

    Purpose

    For knock-in donor vector by CRISPR/CAS system

    Depositor

    Rudolf Jaenisch

    Article

    Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    CRISPR

    Tags

    GFP

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRcCMV Cep215 (Nigg CW493)

    Plasmid

    #41152

    Depositor

    Erich Nigg

    Article

    Graser et al J Cell Sci. 2007 Dec 15;120(Pt 24):4321-

    Insert

    Cep215 (CDK5RAP2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-mCherry

    Plasmid

    #36084

    Purpose

    3rd generation lentiviral plasmid expressing mCherry

    Depositor

    Pantelis Tsoulfas

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-mitoGFP

    Plasmid

    #44385

    Depositor

    Pantelis Tsoulfas

    Article

    Kitay et al Hum Mol Genet. 2013 Jan 24.

    Insert

    mitoGFP (COX8A Human)
    

    Use

    Lentiviral

    Tags

    Cox8 targeting sequence and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-GFP(1-10)

    Plasmid

    #70219

    Purpose

    Expresses GFP(1-10) in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    GFP(1-10)
    

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-GRAB-gDA3h

    Plasmid

    #208699

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3h
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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