8,801 results

1. phND2-N174

   Plasmid

   #31822

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   NeuroD2 (NEUROD2 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-AML1/ETO-IRES-GFP

   Plasmid

   #60832

   Purpose

   AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   AML1/ETO (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-FAH-rtTA3G

   Plasmid

   #120309

   Purpose

   An AAV vector that expresses rtTA3G under the c-fos promoter

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   rtTA3G
   

   Use

   AAV

   Promoter

   c-fos

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-PML IV/pRK5

   Plasmid

   #59742

   Purpose

   Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

   Depositor

   Xiaolu Yang

   Article

   Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

   Insert

   PML isoform IV (PML Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-nCas9n

   Plasmid

   #47929

   Purpose

   expression of an optimized Cas9 for genome-editing in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   nls-zcas9-nls
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4-ERSE1-luc2P-Hygro

   Plasmid

   #101789

   Purpose

   Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

   Depositor

   Seiichi Oyadomari

   Article

   Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

   Insert

   3xERSE-I
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   minimal TATA-box promoter with low basal activity

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-BRAF-V600E

   Plasmid

   #116204

   Purpose

   Lentiviral expression of BRAF V600E

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Mutation

   V600E

   Available Since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. rAAV2/cMG

   Plasmid

   #184539

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-cMG capsid
   

   Use

   AAV; Helper vector for packaging

   Mutation

   changes on AAV9 capsid: K449R, A589D; insertion o…

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

   Plasmid

   #89347

   Purpose

   2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   Jurkat TCRalpha/beta alleles and CD3e and CD3z
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK170.AAV-TRE-Cre-WPRE (Supernova)

    Plasmid

    #85040

    Purpose

    For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nlsCre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-FLAG

    Plasmid

    #46956

    Purpose

    Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    FLAG tag
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEP4 E02S ET2K

    Plasmid

    #20927

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; SV40LT and Klf4 (SOX2, POU5F1, KLF4 Human)
    

    Use

    Episomal/ebna

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUGW-PK-hLC3

    Plasmid

    #61460

    Purpose

    Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

    Depositor

    Isei Tanida

    Article

    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

    Insert

    PK-hLC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-8xHis-Ub

    Plasmid

    #107392

    Purpose

    Mammalian expression 8xHis-Ub

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    8 tandem repeats of 6xHis-Ub (See comments below)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMV306hsp+LuxG13

    Plasmid

    #26161

    Depositor

    Siouxsie Wiles, Brian Robertson

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-GAL4-ERT2-VP16

    Plasmid

    #82588

    Purpose

    Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

    Depositor

    Kryn Stankunas

    Article

    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

    Insert

    GAL4-ERT2-VP16
    

    Promoter

    None

    Available Since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. eGFP-ova-C1

    Plasmid

    #163524

    Purpose

    Expresses both eGFP and chicken ovalbumin in mammalian cells

    Depositor

    Thomas Weber

    Insert

    enhanced-GFP-chicken ovalbumin tandem
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4-ERSE2-luc2P-Hygro

    Plasmid

    #101790

    Purpose

    Luciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xERSE-II
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-NORAD

    Plasmid

    #120383

    Purpose

    Expression vector for lncRNA NORAD

    Depositor

    Igor Ulitsky

    Article

    Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

    Insert

    NORAD (NORAD Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-PE2-P2A-hMLH1dn

    Plasmid

    #174827

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-hMLH1dn
    

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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