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pDV05.0_pEF1a-iRFP720

Plasmid

#200407

Purpose

Expresses iRFP720 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hOKMS

Plasmid

#51543

Purpose

Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

Depositor

Tarjei Mikkelsen

Article

Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

Insert

hOKMS (MYC, KLF4, SOX2, POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B )

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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PB-GFP-Gal8

Plasmid

#127191

Purpose

Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Insert

GFP-Gal8 (LGALS8 Human)

Tags

Gal8 is fused to the c-terminus of GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Promoter

P23

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-Bsd-CMV>tTS/rtTA:T2A:{cymR}

Plasmid

#236251

Purpose

Blasticidin selected lentiviral vector with Tet-On regulatory proteins and the cumate repressor, for use with dual-inducible CymR-CuO system

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

tTS/rtTA
cymR

Use

Lentiviral

Expression

Mammalian

Mutation

rTS and rtTA_M2 linked by T2A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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mTurquoise2

Plasmid

#198196

Purpose

Spectral correction: Expression of mTurquoise2

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mTurquoise2

Expression

Mammalian

Mutation

A206K

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast FAPwt

Plasmid

#210643

Purpose

Expresses the FAP (Fibroblast Activation Protein) protein.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

none

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEcCas-2.0

Plasmid

#190697

Purpose

Constitutive expressing of Cas9 and inducible expression of lambda Red

Depositor

Sheng Yang

Article

Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

Insert

Cas9 and Lamda Red system

Expression

Bacterial

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO mTRPM7

Plasmid

#45482

Depositor

Andrew Scharenberg

Article

Nadler et al Nature. 2001 May 31;411(6837):590-5.

Insert

TRPM7 (Trpm7 Mouse)

Use

Tetracycline inducible

Tags

Flag

Expression

Mammalian

Promoter

Tetracycline-controlled CMV

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
AAV pCAG-FLEX-EGFP-WPRE

Plasmid

#51502

Purpose

Can be used to generate AAV virus that will express EGFP in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EGFP

Use

AAV

Promoter

CAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pME-QFGal4-SV40pA

Plasmid

#155118

Purpose

Middle entry vector containing QFGal4

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4

Use

Gateway middle entry vector

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pV1093

Plasmid

#111428

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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EBFP2-H2B-6

Plasmid

#55243

Purpose

Localization: Nucleus/Histones, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

EBFP2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pOPINK-Cezanne (OTU, aa 53-446)

Plasmid

#61581

Purpose

Expresses human Cezanne (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne (OTUD7B Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-52 and aa 447-843.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158652

Purpose

making transgenics expressing a UAS driven eGFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ9715: pHR-PGK-SV40_NLS-dCasMINI-V4-NFZ-c-Myc_NLS-mCherry-WPRE

Plasmid

#203221

Purpose

Expression of dCasMINI-V4-NFZ-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-NFZ-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-SOD2-2A-Catalase-WPRE

Plasmid

#67635

Purpose

AAV vector expressing both SOD2 and mitochondrial targeted Catalase

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

SOD-2A-Catalase (Sod2, Cat Mouse)

Use

AAV

Mutation

Deleted the Peroxisome Targeting Signal from Cata…

Promoter

CMV

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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