165,385 results

1. pMC-9-35S-11

   Plasmid

   #197759

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   35S terminator
   

   Expression

   Plant

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEvol-pAzFRS.2.t1

   Plasmid

   #73546

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAzFRS.2.t1
   pAzFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAH750

   Plasmid

   #91212

   Purpose

   protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   2 gRNAs targeting tomato ARF8A
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRsetB-CitA-Tq-LR

   Plasmid

   #241830

   Purpose

   Bacterial expression vector for CitA-Tq-LR (low-range)

   Depositor

   Gary Yellen

   Article

   Rosen et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

   Insert

   CitA-Tq-LR
   

   Tags

   7xHis

   Expression

   Bacterial

   Available Since

   Oct. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRsetB-CitA-Tq-HR

   Plasmid

   #241831

   Purpose

   Bacterial expression vector for CitA-Tq-HR (high-range)

   Depositor

   Gary Yellen

   Article

   Rosen et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

   Insert

   CitA-Tq-HR
   

   Tags

   7xHis

   Expression

   Bacterial

   Available Since

   Oct. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pvPE-V4

   Plasmid

   #240288

   Purpose

   Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.

   Depositor

   Lusheng Huang

   Article

   Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

   Insert

   pvPE-V4
   

   Use

   CRISPR

   Tags

   SV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLR02_pXL001_HLAEB2M

   Plasmid

   #131087

   Purpose

   Overexpression of HLA-E fused to B2M

   Depositor

   Xiaojun Lian

   Insert

   HLA-E and B2M with flexible linker
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBHM2678

   Plasmid

   #241723

   Purpose

   Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BLE marker for selection on phleomycin

   Depositor

   Hiten Madhani

   Article

   Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Sept. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBHM2680

   Plasmid

   #241725

   Purpose

   Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSR marker for selection on blasticidin S

   Depositor

   Hiten Madhani

   Article

   Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Sept. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. TwinStrepSUMO-pvc10-Cas9

    Plasmid

    #243755

    Purpose

    For affinity purification of Pvc10 fused to Cas9

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

    Insert

    pvc10-Cas9
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. TwinStrepSUMO-Ad5-SpyCatcher

    Plasmid

    #243751

    Purpose

    For affinity purification of A5 knob fused to SpyCatcher

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

    Insert

    Ad5-SpyCatcher
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9

    Plasmid

    #243744

    Purpose

    PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTD

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

    Insert

    pvc13-Ad5_HUHe-Pvc10-Cas9
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAWP78-PVCpnf_pvc13-Ad5_pvc10-HUHe

    Plasmid

    #243745

    Purpose

    PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on CTD

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

    Insert

    pvc13-Ad5_pvc10-HUHe
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAWP78-PVCpnf_pvc13-SpyTag_Δpvc10

    Plasmid

    #243740

    Purpose

    PVC structural operon - tail fiber (Pvc13) tagged with SpyTag - deleted spike tip (Pvc10)

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

    Insert

    pvc13-SpyTag_Δpvc10
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKOS6b-KOpstS

    Plasmid

    #237503

    Purpose

    Knockout of pstS

    Depositor

    Buz Barstow

    Insert

    phosphate ABC transporter substrate-binding protein PstS
    

    Expression

    Bacterial

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKOS6b-URP112

    Plasmid

    #237504

    Purpose

    Upregulation of mgdh with P112

    Depositor

    Buz Barstow

    Insert

    membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
    

    Expression

    Bacterial

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLIB-Cyclin K

    Plasmid

    #231718

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CCNK (CCNK Human)
    

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmNeon Green-N1-ARF1-R

    Plasmid

    #202430

    Purpose

    Mammalian expression vector containing mNeonGreen-tagged ARF1

    Depositor

    David Bryant

    Article

    Sandilands et al J Cell Biol. 2023 Apr 3;222(4):e20220611

    Insert

    ARF1 (ARF1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57KAN-20XV5

    Plasmid

    #233117

    Purpose

    20X V5 tag multimer

    Depositor

    Steve Stowers

    Insert

    V5 tag
    

    Use

    Unspecified

    Mutation

    None

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57KAN-40XV5

    Plasmid

    #233123

    Purpose

    40X V5 tag multimer

    Depositor

    Steve Stowers

    Insert

    V5 tag
    

    Use

    Unspecified

    Mutation

    None

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pscAAV.CAG-EGFP-SV40pA-T7-T3-BC(p1-14)

    Plasmid

    #231346

    Purpose

    Self complementary AAV genome with components for tracking self-complementary AAV genomes via AAV-Zombie. Also expresses EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7/T3-BC(p1-14)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJC224

    Plasmid

    #231169

    Purpose

    Empty Module A plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids

    Depositor

    Daniel Voytas

    Type

    Empty backbone

    Use

    Subcloning vector

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJC225

    Plasmid

    #231170

    Purpose

    Empty Module B plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids

    Depositor

    Daniel Voytas

    Type

    Empty backbone

    Use

    Subcloning vector

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. HA-CDC25C

    Plasmid

    #229410

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    CDC25C (CDC25C Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pIN1 (gpdA-cTerm-mGL-ptrA)

    Plasmid

    #188735

    Purpose

    Expresses mGreenlantern for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Promoter

    gpdA

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pIN2 (gpdA-nTerm-mGL-ptrA)

    Plasmid

    #188736

    Purpose

    Expresses mGreenlantern for N-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. dCas9 fused to scFv (anti-spike)

    Plasmid

    #186421

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-scFv fusion (anti-SARS-CoV-2 spike)
    

    Use

    CRISPR

    Tags

    6His

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. 1M-Mx-FLAG

    Plasmid

    #206941

    Purpose

    Mammalian Expression of 1M-Mx-FLAG EMcapsulins

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, MxEnc
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 1M-Tm-BC2

    Plasmid

    #206943

    Purpose

    Mamamlian Expression of 1M-Tm-BC2Tag EMcapsulins

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, TmEnc
    

    Tags

    BC2

    Expression

    Mammalian

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pNIC28-SENP1

    Plasmid

    #196354

    Purpose

    protein expression of the His-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP1 catalytic domain
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    contains only E419-L644

    Promoter

    T7

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pVITRO1-mCherry-LaminB1

    Plasmid

    #186348

    Purpose

    Nuclear membrane-targeted mCherry expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    mCherry-LaminB1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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32. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

    Plasmid

    #169550

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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33. miniABEMax V82G nSaCas9

    Plasmid

    #135365

    Purpose

    Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    miniABEMax V82G nSaCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    (V82G)

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA5-F-Med26-NTD R61A K62A

    Plasmid

    #49256

    Purpose

    Mammalian expression of flag-tagged human Med26-NTD R61A K62A

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    NTD R61A K62A

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA5-F-Med26-NTD

    Plasmid

    #49255

    Purpose

    Mammalian expression of flag-tagged Med26-NTD

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    NTD (aa 1-339)

    Available Since

    Dec. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA5-F-Med26-R61A K62A

    Plasmid

    #49254

    Purpose

    Mammalian expression of flag-tagged human Med26 R61A K62A

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med26 (MED26 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R61A K62A

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pLNHA-C1-HsXRN1

    Plasmid

    #66596

    Purpose

    expresses lambdaN-HA-tagged XRN1 in mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    XRN1 (XRN1 Human)
    

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

    Plasmid

    #175529

    Purpose

    Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Cox8a-HaloTag7
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-PE2-SpRY

    Plasmid

    #159979

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpRY
    

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GSK3beta  (1015)

    Plasmid

    #49491

    Purpose

    expresses human Gsk3beta

    Depositor

    Scott Friedman

    Article

    Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

    Insert

    GSK3beta (GSK3B Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge