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We narrowed to 10,180 results for: yeast

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Showing: 4701 - 4750 of 10180 results

  3. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  5. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  6. pIFU002

    Plasmid
    #174299
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Insert
    Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, N501Y
    Available Since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    174299-plasmid-map-sequence-id-342196

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  7. pIFU001

    Plasmid
    #174298
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Insert
    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, E484K
    Available Since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    174298-plasmid-map-sequence-id-342193

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  10. pJC20cdc8.110

    Plasmid
    #99363
    Purpose
    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.
    Depositor
    Dan Mulvihill
    Article
    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.
    Insert
    cdc8 (cdc8 Fission Yeast)
    Expression
    Bacterial
    Mutation
    Alanine 18 to Threonine and Glutamic acid 31 to L…
    Promoter
    T7
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    99363-plasmid-map-sequence-id-192456

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  12. pV1093

    Plasmid
    #111428
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111428-plasmid-map-sequence-id-212463

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  32. pYCTK034

    Plasmid
    #176738
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Saccharomyces cerevisiae (barrier part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    bar1Sc
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176738-plasmid-map-sequence-id-351575

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  33. pYCTK027

    Plasmid
    #176731
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Saccharomyces cerevisiae (receiver part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    ste2Sc
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176731-plasmid-map-sequence-id-351583

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  34. pYCTK002

    Plasmid
    #176706
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter FUS1 of S. cerevisiae (response part)
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    P-FUS1
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176706-plasmid-map-sequence-id-351547

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Showing: 4701 - 4750 of 10180 results