We narrowed to 18,356 results for: Tec
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pDisplay FLIPE-100u
Plasmid#13547DepositorInsertFLIP-E S118W
UseTagsECFP and VenusExpressionMammalianMutationS118W in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
YIp211-P30-His-pol30(K127/164R)-3'
Plasmid#99549PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationK127R, K164RPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pJL1-PorA-4xDQNAT
Plasmid#128392PurposeIn vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sitesDepositorInsertPorA
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-TTc-4xDQNAT
Plasmid#128393PurposeIn vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sitesDepositorInsertTetanus toxin fragment C domain
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-GLICNot
Plasmid#140182PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and NotI siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagsGSTExpressionMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ3
Plasmid#83558PurposeUsed to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.DepositorInsertGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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mKO-Golgi-7
Plasmid#57843PurposeLocalization: Golgi Complex, Excitation: 548, Emission: 561DepositorInsertGolgi (B4GALT1 Human)
UseTagsmKOExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Cas9-L2
Plasmid#141038PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Gal4pBS4-Pleum
Plasmid#60329PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pME-BTK-PH
Plasmid#67673PurposeGateway entry clone containing BTK-PH with no stop codonDepositorInsertBTK-PH-NS
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc
Plasmid#141102Purposebioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosisDepositorInsertSEP-Nanoluc
UseLentiviralTagsExpressionMutationPromoterEF1αAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pconst #3-RFP
Plasmid#81077Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23101Available sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11a-TAX-L10-STEPtag
Plasmid#159375PurposeExpresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.DepositorInsertTAX-L10-STEPtag
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-STEPtag-L10-TAX
Plasmid#159374PurposeExpresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.DepositorInsertSTEPtag-L10-TAX
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pUPD2-ODPAMTS (12)
Plasmid#101694PurposeODPAMTS in domestication vectorDepositorInsertCCAT-[MTS from S. cerevisiae Pyruvate dehydrogenase E1 component subunit alpha (YER178W)]-AATG
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-ODPBMTS (12)
Plasmid#101695PurposeODPBMTS in domestication vectorDepositorInsertCCAT-[MTS from S. cerevisiae Pyruvate dehydrogenase E1 component subunit beta (YBR221C)]-AATG
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCF3
Plasmid#67110PurposeStandard for cell-free expression benchmarking.DepositorInsertTCF3 (TCF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationTruncated isoformPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2
Plasmid#141052PurposeMutisite Gateway mediated vector constructionDepositorInserteSpCas9(1.1)-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
GWF303
Plasmid#133611PurposeCD95-1-2xPP7, NLS-PCP-DNCR2-P2a-BFPDepositorInsertCD95-1-2xPP7, PCP-DNCR2, BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GWF314
Plasmid#133610PurposeCXCR4-com, NLS-com-GNCR1-P2a-BFPDepositorInsertCXCR4-6-com, com-GNCR1, BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-GagB500
Plasmid#46314DepositorInsertsp19
GagB500 DNA-1
linker
GagB500 DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
psecBFP2
Plasmid#62230Purposeencodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.DepositorInsertsecBFP2
UseTagssecbfp2ExpressionMammalianMutationPromotercmvAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
6his R.3-AR GUSpET28a+/pLysS
Plasmid#61157PurposeN-terminal His tagged round 3 aldehyde-resistant GUS expression vectorDepositorInsertGUSAR
UseTags6x HisExpressionBacterialMutationN66D, D151N, A219V, I396T, T480A, Q498R, D508E an…PromoterT7Available sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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Park2_L (TAL 3012)
Plasmid#36004DepositorInsertTAL array targeting Park2 (prkn Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
Park2_R (TAL 3013)
Plasmid#36005DepositorInsertTAL array targeting Park2 (prkn Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBSWHhc
Plasmid#133901PurposeExpression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWHDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 YWHAB
Plasmid#67063PurposeStandard for cell-free expression benchmarking.DepositorInsertYWHAB (YWHAB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pconst #2-RFP
Plasmid#81076Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter- BBa_J23104Available sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
SEPT11 sh2 shRNA
Plasmid#122023PurposeKnock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.DepositorInsertSEPT11 shRNA (Sept11 Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0006
Plasmid#42246PurposegRNA targeted to zebrafish gene rgs4DepositorInsertgRNA-rgs4 (rgs4 Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z globin NB Probe
Plasmid#120390PurposeAllows in vitro SP6 Polymerase driven transcription of a beta globin northern blot probeDepositorInsertbeta globin partial 3' UTR
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Yg
Plasmid#61655PurposeGateway vector for generating yellow fluorescent protein fusions in fungiDepositorInsertenhanced yellow fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGC4785
Plasmid#113409PurposeTranslation reporter with inducible translation coupling (low native GFP expression, high under coupling)DepositorInsert359_31121113_2
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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CP1300-CBEmax
Plasmid#119805PurposeC-to-T base editorDepositorInsertrApobec1-nSpCas9 (CP1300) -UGI-UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
ptp4a3_L (TAL 3000)
Plasmid#35992DepositorInsertTAL array targeting ptp4a3 (ptp4a3a Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pATI (pKD-G7)
Plasmid#62686PurposeMammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP123/amiR-eGFP419
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC4-eGFP
Plasmid#107410PurposeThis plasmid contain promoter PthrC4, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPthrC4Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only
