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pCR-blunt-TOPO-FNDC5

Plasmid

#35970

Depositor

Bruce Spiegelman

Article

Bostrom et al Nature. 2012 Jan 11;481(7382):463-8. doi

Insert

fibronectin type III domain containing 5 (Fndc5 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
XE143 sFRP-3-CS2+

Plasmid

#16691

Depositor

Randall Moon

Insert

sFRP-3 (Frzb Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
pCR-HA-IKKalpha

Plasmid

#15469

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha (Chuk Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP_WT-talin1 (1-2541)

Plasmid

#166112

Purpose

For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

WT-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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EF.mHES1.Ubc.GFP

Plasmid

#17625

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (Hes1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pCruzHA SIRT1 G261A

Plasmid

#10963

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

G261A

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
8798 GFP-KrasG12C_2B_hygro

Plasmid

#64376

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

changed glycine 12 to cysteine

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-7 [N183/15R]

Plasmid

#177507

Purpose

Mammalian Expression Plasmid of anti-QKI-7 (Human). Derived from hybridoma N183/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-QKI-7 (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-CD4-TLR4 cyto

Plasmid

#133064

Purpose

Retroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

CD4-TLR4cyto (Tlr4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

Plasmid

#101298

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Use

Tags

Expression

Mammalian

Mutation

Contains amino acids 1-698 of alpha-Catenin

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-GFP-TLR4 delta tail

Plasmid

#133063

Purpose

Retroviral expression of mouse TLR4 with out its cytosolic tail

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

TLR4 delta tail (Tlr4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDendra2-H2a

Plasmid

#157816

Purpose

Fluorescent reporter for histone H2A tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H2A (H2ac13 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8_Flag2-Blast

Plasmid

#175038

Purpose

Mammalian expression of full length mMPP8 with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE MDER

Plasmid

#13494

Depositor

Stephen Tapscott

Article

Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

Insert

MyoD1-Estrogen Receptor Fusion Protein (Myod1 Human, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

full length MyoD was fused to aa282-595 of Esr1. …

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV5-ALK6-HA

Plasmid

#11769

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK6 (Bmpr1b Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pCI-neo-μ1A-(HA)3

Plasmid

#198177

Purpose

Expression of HA-tagged AP-1 μ1A in mammalian cells

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1A

Use

Tags

triple HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_Sik3_T142Q

Plasmid

#119973

Purpose

Lentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Sik3 (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

change Threonine 142 to Glutamine

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pOC2 Tubb3-GFP KI

Plasmid

#183432

Purpose

CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-GPD1L

Plasmid

#32486

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L (Gpd1l Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pBa-FRB-3myc-KIF1B beta tail 387-1770

Plasmid

#64287

Purpose

KIF1B beta aa 387-1770 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF1B beta (Kif1b Mouse, Rat)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa 387-1770, small portion of 5'end matches …

Promoter

chicken Beta Actin

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

Plasmid

#156442

Purpose

For transient expression of mouse PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5B (Pds5b Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mTSP1-luciferase

Plasmid

#12409

Depositor

Paul Bornstein

Insert

mTSP1 promoter (Thbs1 Mouse)

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2006

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-cMyc

Plasmid

#136614

Purpose

Dox-inducible lentiviral vector expressing mouse cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

cMyc (Myc Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pQCi-mB2m-sgRNA

Plasmid

#154090

Purpose

pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA targeting murine B2M locus

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mPA-GFP-MAP4-C-10

Plasmid

#57145

Purpose

Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

MAP4

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCAG-nDsRedFL-miR9/9*

Plasmid

#177805

Purpose

The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Agarabi CHO Light Chain in pJ201

Plasmid

#111566

Purpose

Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

Depositor

Cyrus Agarabi

Article

Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

Insert

Murine light chain

Use

Synthetic Biology; Cloning vector

Tags

Expression

Mutation

Promoter

None

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mGLDC

Plasmid

#72881

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

Gldc (Gldc Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
Unc5b.a-Fc-His

Plasmid

#72178

Purpose

Expresses the extracellular region of the Unc5B, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5b.a (Unc5b Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL K-ras 3'UTR

Plasmid

#14804

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

K-ras 3'UTR (Kras Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pFlox_N-p300_BSD_FKBPF36V_EYFP

Plasmid

#163885

Purpose

Acute Depletion P300-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Ep300 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS mNFATc3-EE

Plasmid

#17868

Depositor

Jerry Crabtree

Article

Graef et al Cell. 2001 Jun 29. 105(7):863-75.

Insert

NFATc3 (Nfatc3 Mouse)

Use

Tags

EE

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
Anti-GluN2A [N327A/38R]

Plasmid

#177532

Purpose

Mammalian Expression Plasmid of anti-GluN2A/NR2A glutamate receptor (Rat). Derived from hybridoma N327A/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GluN2A/NR2A glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grin2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-H3.3-N-14

Plasmid

#54116

Purpose

Localization: Nucleus/Histones, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

H3.3 (H3f3b Mouse)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-IRES-hrGFP-mWnt5a

Plasmid

#89772

Purpose

Expresses mouse Wnt5a in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Rodriguez et al J Neurosci. 2014 Jul 30;34(31):10285-97.

Insert

mouse Wnt5a (Wnt5a Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV eIF4E W73A IRES GFP

Plasmid

#18762

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E W73A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

W73A

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
8047_GFP-Kras2B_ires_puro

Plasmid

#64371

Purpose

This a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

none

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
3539 pMIG Bax

Plasmid

#8788

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bax (Bax Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
Src(WT)-SsrA(Y7I)-mCer3

Plasmid

#220998

Purpose

This is microTag (Y7I mutation in SsrA)-Src with mCerulean3 at C-terminal

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA, mCerulean3 (Src Mouse)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Y7I in SsrA

Promoter

CMV

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-miRFP713

Plasmid

#197208

Purpose

Expresses the protein of miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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