4,129 results

1. pdCas9-humanized

   Plasmid

   #44246

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dead Cas9 with 3X NLS
   

   Use

   CRISPR and Retroviral; Vector

   Tags

   3x NLS

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCGN-SRF

   Plasmid

   #11977

   Purpose

   For mammalian expression of human SRF with an HA tag

   Depositor

   Ron Prywes

   Article

   Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

   Insert

   SRF (SRF Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE PGK-GFP-IRES-LUC-W

   Plasmid

   #46793

   Purpose

   2nd generation lentiviral vector

   Depositor

   Darrell Kotton

   Article

   Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

   Inserts

   PGK
   eGFP (eGFP )
   IRES
   Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSERT pcDNA3

   Plasmid

   #15483

   Depositor

   Randy Blakely

   Article

   Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

   Insert

   hSERT (SLC6A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRK669

   Plasmid

   #71313

   Purpose

   Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

   Depositor

   Todd Klaenhammer, Rodolphe Barrangou

   Article

   Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-myc3-bTrCP

   Plasmid

   #20718

   Depositor

   Yue Xiong

   Article

   Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

   Insert

   beta-transducin repeat containing (BTRC) (Btrc Mouse)
   

   Use

   Tags

   Myc3

   Expression

   Mammalian

   Mutation

   *

   Promoter

   Available since

   May 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. mApple-N1

   Plasmid

   #54567

   Purpose

   Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY004 (pcDNA3.1-hFnCpf1)

   Plasmid

   #69976

   Purpose

   Expresses humanized FnCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hFnCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. T4 Lysozyme WT*

   Plasmid

   #18111

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No cysteines: C54T, C97A

   Promoter

   Available since

   Aug. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. TagRFP-T-EEA1

    Plasmid

    #42635

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-LKB1

    Plasmid

    #21147

    Depositor

    Junying Yuan

    Article

    Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

    Insert

    GFP-LKB1 (Stk11 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pLYS1-FLAG-MitoGFP-HA

    Plasmid

    #50057

    Purpose

    Expression of GFP targeted to mitochondria with Flag and HA tags

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    Mito-GFP
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST pcDNA5-FRT/TO-eGFP-Rif1

    Plasmid

    #52506

    Purpose

    mammalian expression vector of Rif1 siRNA resistant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    RIF1 (RIF1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: ATAGTAC

    Promoter

    Available since

    June 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pRSET Nucleolin

    Plasmid

    #13037

    Depositor

    Peter Newburger

    Article

    Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-PGC1

    Plasmid

    #4

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Cell 1998 Mar 20;92(6):829-39.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. G-CaMP3

    Plasmid

    #22692

    Purpose

    a single-wavelength GCaMP2-based genetically encoded calcium indicator

    Depositor

    Loren Looger

    Article

    Tian et al Nat Methods. 2009 Dec;6(12):875-81.

    Insert

    G-CaMP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N363D mutation (increased fluorescence)

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid

    #50458

    Purpose

    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    rM3D-mCherry
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

    Plasmid

    #20299

    Purpose

    Cre-activated AAV expression of mCherry

    Depositor

    Karl Deisseroth

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-n1-APP

    Plasmid

    #69924

    Purpose

    mammalian expression of human APP fused to EGFP

    Depositor

    Thomas Wassmer, Zita Balklava

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    APP (APP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDZ Flag USP28

    Plasmid

    #15665

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    USP28 (USP28 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pHAGE EF1α dCas9-VP64

    Plasmid

    #50918

    Purpose

    Constitutive dCas9-VP64 lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-myc3-CUL1

    Plasmid

    #19896

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 1 (CUL1 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSETa mEos2

    Plasmid

    #20341

    Purpose

    bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

    Depositor

    Loren Looger

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    mEos-2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HDAC8 Flag

    Plasmid

    #13825

    Depositor

    Eric Verdin

    Article

    Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

    Insert

    HDAC8 (HDAC8 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAVS1-TLR targeting vector

    Plasmid

    #64215

    Purpose

    Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    traffic light reporter (TLR) (AAVS1 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    see publication for details

    Promoter

    CAG

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLOX-CWBmi1

    Plasmid

    #12240

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    CMV promoter upstream of mBmi-1. LoxP in 3' …

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti PGK Blast V5-LUC (w528-1)

    Plasmid

    #19166

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

    Plasmid

    #26966

    Purpose

    AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    eNpHR 3.0
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    ER export at 3' end of gene. Trafficking sig…

    Promoter

    EF1alpha

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV_TRET_hNgn2_UBC_Puro

    Plasmid

    #61474

    Purpose

    3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn2 (NEUROG2 Human)
    Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. -1748 human cyclin D1 promoter pGL3Basic

    Plasmid

    #32726

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase; Reporter gene

    Tags

    Expression

    Mutation

    Promoter

    -1748CCND1 promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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