178,651 results

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pAAV-PTRE-tight-hM3Dq-mCherry

Plasmid

#66795

Purpose

Expresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging

Depositor

William Wisden

Publication

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

pTIGHT promoter-hM3Dq-mCherry

Use

AAV

Expression

Mammalian

Promoter

pTIGHT TRE

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Publication

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Mutation

3' UTR only

Promoter

SP6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET-dCas9-VP64-6xHis

Plasmid

#62935

Purpose

Expression of dCas9-VP64-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

dCas9-VP64

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A and H840A amino acid changes render Cas9 nuc…

Promoter

T7

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET28a-EGFP-CNA35

Plasmid

#61603

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with EGFP

Depositor

Maarten Merkx

Publication

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

EGFP and CNA35

Tags

His tags

Expression

Bacterial

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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DsRed2-N1

Plasmid

#54493

Purpose

Localization: N1 Cloning Vector, Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Tags

DsRed2

Expression

Mammalian

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

8 Articles

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DRH334: FCK-QuasAr2-mO2

Plasmid

#51692

Purpose

Fluorescent reporter of membrane voltage with improved sensitivity

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CaMKIIa

Available since

April 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-ReaChR-citrine

Plasmid

#50954

Purpose

ReaChR-citrine in AAV2 vector under human synapsin promoter

Depositor

Roger Tsien

Publication

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

AAV

Tags

citrine

Promoter

truncated human Synapsin promoter

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pGFP-Cytochrome C

Plasmid

#41181

Depositor

Douglas Green

Publication

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cytochrome C (CYCS Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEGFP-hsp27 wt FL

Plasmid

#17444

Depositor

Andrea Doseff

Publication

Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

small heat shock protein27 (HSPB1 Human)

Tags

GFP

Expression

Mammalian

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pFA6a-link-yomRuby2-SpHis5

Plasmid

#44858

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby2

Expression

Yeast

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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CMV500 A-CREB

Plasmid

#33371

Depositor

Charles Vinson

Publication

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CREB (Creb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available since

Dec. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Flag-TRIM24

Plasmid

#28138

Depositor

Michelle Barton

Publication

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Ai6

Plasmid

#22798

Depositor

Hongkui Zeng

Publication

Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Insert

CAG-Floxed ZsGreen

Use

Mouse Targeting

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pENTR/pSUPER+ (575-1)

Plasmid

#17338

Purpose

Gateway entry vector for shRNA expression under H1 promoter

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and RNAi

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3.1/hChR2-EYFP

Plasmid

#20939

Purpose

Mammalian expression of humanized ChR2 fused to EYFP

Depositor

Karl Deisseroth

Publication

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

channelrhodopsin-2

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRK5-HA GST RagB wt

Plasmid

#19301

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAd-Track Flag-SIRT1

Plasmid

#8438

Depositor

Pere Puigserver

Publication

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3 hE47

Plasmid

#16059

Depositor

Robert Benezra

Publication

Jen et al Genes Dev. 1992 Aug . 6(8):1466-79.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E47 (TCF3 Human)

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Rc/CMV cyclin A

Plasmid

#8959

Depositor

Bob Weinberg

Publication

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cyclin A (CCNA2 Human)

Expression

Mammalian

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mini-pHelper-1.0

Plasmid

#230933

Purpose

The mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

Depositor

Qizhao Wang

Publication

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Type

Empty backbone

Use

AAV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3 Basic Vector

Plasmid

#212936

Purpose

Firefly luciferase vector for investigating regions controlling transcription

Depositor

Debrya Groskreutz

Type

Empty backbone

Use

Luciferase

Mutation

WT

Available since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

39 Articles

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pLJM1-FIRE-pHLy

Plasmid

#170775

Purpose

Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

Depositor

Aimee Kao

Publication

Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

Insert

LAMP1 (LAMP1 Human)

Use

Lentiviral

Tags

mCherry and mTFP1

Expression

Mammalian

Mutation

84 nucleotide Signal Sequence switched to the beg…

Promoter

CMV

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET51b(+)_EGFP_NbALFA

Plasmid

#136626

Purpose

Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDEST-CMV-N-mCherry

Plasmid

#123215

Purpose

N-terminal mCherry tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTP1005

Plasmid

#104160

Purpose

Bacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PMXS-NDI1

Plasmid

#72876

Purpose

expresses NDI1 in mammalian cells

Depositor

David Sabatini

Publication

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

NDI1 (NDI1 Budding Yeast)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Plasmid

#64512

Purpose

Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XmTagBFP2

Expression

Mammalian

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

Plasmid

#62723

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has service

AAV1 and AAV5

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

human synapsin promoter

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

71 Articles

1 Data Hub Report

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pLH-nmsgRNA1.1

Plasmid

#64115

Purpose

Vector for expression of Nm sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Publication

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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