We narrowed to 18,364 results for: Tec
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Plasmid#215251PurposeTU for the expression of the CIB1 gene fused to the VPR activation domain under the P35S promoter and TNOS terminator in alpha1DepositorInsertP35S:CIB1:VPR:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3473
Plasmid#215246PurposeCDS of Luz gene from N. nambi codon optimized for N.benthamiana. This gene generate the luminiscence of the bioluminiscente pathwayDepositorInsertLuz
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3472
Plasmid#215245PurposeCDS of H3H from N. nambi codon optimized for N.benthamiana. This gene is the second step of the bioluminiscente pathwayDepositorInsertH3H
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMJ074
Plasmid#215654PurposepET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143DepositorInsertYellow fluorescent protein
UseSynthetic BiologyTags10x histidine tagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-TtrpC
Plasmid#213467PurposeVector containing the TrpC terminator for Golden Gate cloning in the Fg vectorDepositorInsertTerminator of tryptophan synthase of Aspergillus nidulans
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-PgpdA
Plasmid#213466PurposeVector containing the gpdA promoter for Golden Gate cloning in the Fg vectorDepositorInsertPromoter of the glyceraldehyde-3-phosphate dehydrogenase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-PtrpC
Plasmid#213465PurposeVector containing the TrpC promoter for Golden Gate cloning in the Fg vectorDepositorInsertPromoter of tryptophan synthase of Aspergillus nidulans
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD_lambda_mRFP
Plasmid#217831PurposeYeast surface display Fab vector with lambda light chain and mRFPDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD_kappa_mRFP
Plasmid#217830PurposeYeast surface display Fab vector with kappa light chain and mRFPDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMP_lambda
Plasmid#217829PurposeMini-mutagenesis plasmid for lambda light chain YSD FabsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMP_kappa
Plasmid#217828PurposeMini-mutagenesis plasmid for kappa light chain YSD FabsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher (Min)
Plasmid#216713PurposeExpresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014DepositorInsertCys-SpyCatcher (Min)
UseTags6xHisExpressionBacterialMutationSee Li...Howarth, J. Mol. Biol., 2014PromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIDAF
Plasmid#213712PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIBY55
Plasmid#213711PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
β2AR-PPI-CALPon1.0 (APEX)
Plasmid#213406PurposePeroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)DepositorInsertβ2AR-E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-sgRNA-mCherry-Puro sgPIDD
Plasmid#211528PurposeDeletes PIDDDepositorInsertsgPIDD
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK1520 sgRPS3
Plasmid#211526PurposeFacilitate knock-in of RPS3-Keima fusion proteinDepositorInsertsgRPS3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 hygro sgUCK1
Plasmid#211524PurposeDeletes UCK1DepositorInsertsgUCK1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.1
Plasmid#208306PurposeExpresses ABE-RA4.1 in mammalian cellsDepositorInsertTadA-RA4.1-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.2
Plasmid#208307PurposeExpresses ABE-RA4.2 in mammalian cellsDepositorInsertTadA-RA4.2-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.3
Plasmid#208308PurposeExpresses ABE-RA4.3 in mammalian cellsDepositorInsertTadA-RA4.3-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.4
Plasmid#208309PurposeExpresses ABE-RA4.4in mammalian cellsDepositorInsertTadA-RA4.4-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.5
Plasmid#208310PurposeExpresses ABE-RA4.5 in mammalian cellsDepositorInsertTadA-RA4.5-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.6
Plasmid#208311PurposeExpresses ABE-RA4.6 in mammalian cellsDepositorInsertTadA-RA4.6-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.0
Plasmid#208312PurposeExpresses ABE-RA5.0 in mammalian cellsDepositorInsertTadA-RA5.0-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.1
Plasmid#208313PurposeExpresses ABE-RA5.1 in mammalian cellsDepositorInsertTadA-RA5.1-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.3
Plasmid#208314PurposeExpresses ABE-RA5.3 in mammalian cellsDepositorInsertTadA-RA5.3-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.4
Plasmid#208315PurposeExpresses ABE-RA5.4 in mammalian cellsDepositorInsertTadA-RA5.4-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, R47K, P48A, R51L, I76Y, V82S, A106V, D108G,…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.6
Plasmid#208317PurposeExpresses ABE-RA5.6 in mammalian cellsDepositorInsertTadA-RA5.6-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX
Plasmid#209712PurposeMammailan expression of WDR5, SH2, and HRas separated by P2A.DepositorInsertWDR5-P2A-SH2-P2A-Ras-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NLS-MBP-rATOM(WDR5)
Plasmid#209710PurposeMammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signalDepositorInsertNLS-MBP-rATOM(WDR5)
UseTagsNLS-MBPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP765_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IF_lacZ
Plasmid#192409PurposeTo act as a 1-input state of the A NIMPLY B gate.DepositorInsertB3RT-Act2-B3RT::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB312
Plasmid#203569PurposeTU for the expression of Nanoluciferase (Nluc) under the PgpdA promoter.DepositorInsertPgpdA:Nluc:Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXC3cIfm
Plasmid#193750PurposeExpresses frame-shifted CI under the control of PLUX promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertFrame-shifted CI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLUXAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPINA7A2mTFP1(F)
Plasmid#193774PurposeExpresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertmTFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPINA7 synthetic promoter carrying binding sites f…Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Dm4E-T_9-44-F41DW42D-GB1_AA
Plasmid#148256PurposeBacterial Expression of Dm4E-T_9-44-F41DW42DDepositorInsertDm4E-T_9-44-F41DW42D (4E-T Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Dm4E-T_9-44-K12E-GB1_AA
Plasmid#148257PurposeBacterial Expression of Dm4E-T_9-44-K12EDepositorInsertDm4E-T_9-44-K12E (4E-T Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Dm4E-T_9-44-K12ER19E-GB1_AA
Plasmid#148258PurposeBacterial Expression of Dm4E-T_9-44-K12ER19EDepositorInsertDm4E-T_9-44-K12ER19E (4E-T Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Dm4E-T_9-44-K12ER19EK43E-GB1_AA
Plasmid#148259PurposeBacterial Expression of Dm4E-T_9-44-K12ER19EK43EDepositorInsertDm4E-T_9-44-K12ER19EK43E (4E-T Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Dm4E-T_9-44-R19E-GB1_AA
Plasmid#148260PurposeBacterial Expression of Dm4E-T_9-44-R19EDepositorInsertDm4E-T_9-44-R19E (4E-T Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Dm4E-T_9-44-YLFWtoAADD-GB1_AA
Plasmid#148261PurposeBacterial Expression of Dm4E-T_9-44-YLFWtoAADDDepositorInsertDm4E-T_9-44-YLFWtoAADD (4E-T Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIS001
Plasmid#194012PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertROSA-TRE-C16V5-G-pA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP744_L2pV1_OR_B3RT_FRT_Rluc_1IF_lacZ
Plasmid#192388PurposeTo act as a 1-input state of the OR gate.DepositorInsertAct2::FRT-B3RT-OCS-FRT-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIS003
Plasmid#194014PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex4 XTR f1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-eDHFR
Plasmid#194635PurposeMammalian expression of the eDHFR control bait in VirotrapDepositorInsertp55 GAG
UseTagsecDHFRExpressionMammalianMutationPromoterSRalphaAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-eLOV-TEVcs-Gal4
Plasmid#173119PurposeExpresses CCR6 eLOV receiver construct in DIPGDepositorInsertCCR6-eLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-hLOV-TEVcs-Gal4
Plasmid#173120PurposeExpresses CCR6 hLOV receiver construct in DIPGDepositorInsertCCR6-hLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV CMV CCR6-eLOV-TEVcs-Gal4
Plasmid#173113PurposeExpresses CCR6 receiver construct in mammalian cellsDepositorInsertCCR6-eLOV-TEVcs-tTA
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mVenusC-14-HP1b Chromo
Plasmid#191398PurposeHP1b chromo domain fused to C-terminal part of split mVenusDepositorInsertFLAG-mVenusC-14-HP1b Chromo
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-NLS-MaSat ZF-18-mVenusN
Plasmid#191392PurposeZinc finger fused to N-terminal part of split mVenusDepositorInsertFlag-NLS-MaSat ZF-18-mVenusN
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only