10,970 results

1. pRDA_526

   Plasmid

   #184859

   Purpose

   Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

   Insert

   sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCKO_Luciferase_sgRNA

   Plasmid

   #74190

   Purpose

   lentiviral vector expressing sgRNA targeting Luciferase

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   Luciferase sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-AsCas12f-full-length-sgRNA

   Plasmid

   #204638

   Purpose

   Expression of full-length AsCas12f sgRNA in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   full-length AsCas12f sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK473

   Plasmid

   #114715

   Purpose

   sgRNA for LGN's N-terminal locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   LGN sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGH020 sgp53

   Plasmid

   #227947

   Purpose

   sgRNA targeting p53, GFP, neomycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

   Depositor

   Laura Attardi

   Article

   Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

   Insert

   Tp53 (Trp53 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. SGEP

   Plasmid

   #111170

   Purpose

   miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   SFFV

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPRv2_mSTING_scrambled_gRNA_2

   Plasmid

   #196628

   Purpose

   Scrambled control 2 for STING knock-out in murine cells.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING scrambled gRNA 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBA904

   Plasmid

   #122238

   Purpose

   Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA with cs1 in stem loop 2 of the constant region
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1 puro shRNA beta-catenin

   Plasmid

   #18803

   Purpose

   3rd gen lentiviral vector for knocking down beta-catenin gene expression

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   b-catenin shRNA (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 22, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216246

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgAAVS1.5
    

    Use

    CRISPR; Human safe harbor locus (a avs1) site-spe…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

    Plasmid

    #214881

    Purpose

    Lentiviral vector encoding RfxCas13d targeting B2M guide

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crB2M-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

    Plasmid

    #179008

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX601-GFP

    Plasmid

    #84040

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with GFP

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. shCD44-2 pRRL

    Plasmid

    #19123

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against CD44 (CD44 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

    Plasmid

    #179002

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPRv2 sgIRF3#5

    Plasmid

    #127642

    Purpose

    Knock-out of human IRF3

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 sgRNA (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. LT3GEPIR-CCND1shRNA1

    Plasmid

    #220577

    Purpose

    To inducibly knockdown CCND1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

    Insert

    CCND1 shRNA1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1.sh.beta-catenin.1248

    Plasmid

    #19761

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    small hairpin RNA against beta-catenin (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRDA_221

    Plasmid

    #169142

    Purpose

    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

    Depositor

    David Root, John Doench

    Insert

    EGFP , EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge