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  1. Viral Production

    Type
    Collection
    ...of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins...SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed...Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total protein present in the sample. Sterility...vectors consist of full particles (white arrowhead) relative to empty particles (black arrowhead). Scale bar...

  3. Viral Vectors

    Type
    Collection
    ...transfer in clinical trials. These viruses have a relatively high transduction efficiency, though this infectivity...These engineered viral genomes are also modified (relative to natural viruses) for safety through the removal...vector plasmids available at Addgene and other related resources. Special...

  4. Antibody Guide

    Type
    Collection
    ...colored product that accumulates over time which is relative to the amount of enzyme present in each well. ... categories: Quantification - used to quantify relative amounts of protein. Includes: Western blot ELISA... western blots or ELISAs, are used to quantify relative amounts of protein. Western blot Western blots...proteins separated by size on an SDS-PAGE gel. Though relative quantification can be done, western blots primarily...referred to as housekeeping genes). This allows for relative comparison of expression between different samples... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain...

  5. Retrovirus Plasmids

    Type
    Collection
    ...called γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection...

  6. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...contained at lower biosafety levels and elicit relatively low immunological effects in vivo . While AAVs...virus, in order to propagate. This is due to the reliance of the AAV on certain exogenous gene products ...

  7. TALEN Guide

    Type
    Collection
    ...Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into a single...at Massachusetts General Hospital also recently released a TALEN kit through Addgene. This kit, first described...

  8. Rett Syndrome

    Type
    Collection
    ...diagnostic criteria, defined as the presence of relatively normal early development, regression with a loss... of treatments and cures for Rett Syndrome and related MECP2 disorders. The RSRT has a comprehensive, ...new window) PMID: 10508514 Archer et al. 2007. Correlation between clinical severity in patients with Rett...display tissue- and cell-specific differences and correlate with neuronal maturation. Hum Mol Genet . 11, ...

  10. Bacterial Expression Systems

    Type
    Collection
    ...researchers also use bacteria like E. coli and their relatively well understood biology to answer many interesting...

  11. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... for genetic screening experiments, due to the relative ease of designing gRNAs and the ability to modify...3rd 4 2,852 Bonifacino Lab Human ubiquitination-related proteins CRISPR KO library 174592 Knockout Human...

  12. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...of orientation of the protospacer sequence/PAM relative to the top or bottom strand. Determine the reverse...identification, and characterization. This strategy relies on cellular delivery of a pair of chimeric single...limiting dilution prior to performing this step to reliably obtain approximately 30 cells per 96-well plate...coli cells and plate onto LB agar plates with the relevant antibiotic. Select multiple colonies, mini-prep...RNA, perform RT-qPCR for gene expression of the relevant gene 7,8 . To evaluate the protein, perform an...an immunoblot using an antibody against the relevant protein 9 . References This protocol is excerpted...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...to generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail...multiple cell types, but are typically limited to related cell lineages. Pluripotent embryonic and multipotent...technology removes the possible ethical concerns related to embryonic stem cell use and has the major advantage...

  14. Immunology Research Plasmids and Resources

    Type
    Collection
    ...transcript 1L - RELB v-rel reticuloendotheliosis viral oncogene homolog B I-REL, IREL RFX5 regulatory ...RASGRP3 RAS guanyl releasing protein 3 (calcium and DAG-regulated) GRP3, KIAA0846 RELA v-rel reticuloendotheliosis...CALDAG-GEFII, MGC129998, MGC129999, RASGRP, V, hRasGRP1 RELA v-rel reticuloendotheliosis viral oncogene homolog ...like beta FIZZ1, FIZZ2, HXCP2, RELM-beta, RELMb, RELMbeta, XCP2 RLN1 relaxin 1 H1, RLXH1, bA12D24.3.1, bA12D24.3.2...polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related sequence...CST-29 CRH corticotropin releasing hormone CRF CRHR1 corticotropin releasing hormone receptor 1 CRF-R,...ESRRA estrogen-related receptor alpha ERR1, ERRa, ERRalpha, ESRL1, NR3B1 ESRRB estrogen-related receptor beta...

  15. Ras Pathway

    Type
    Collection
    ...guanine nucleotide releasing factor RASGRP RASGRP1 RASGRP2 RASGRP3 RASGRP4 RAS guanyl releasing protein RASSF...for clarity and does not indicate a specific relationship. The content and map for this page were generated...FGFR4 Fibroblast growth factor receptor FLT3 Fms related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase...methyltransferase INSR Insulin receptor INSRR Insulin receptor-related receptor IRS IRS1 IRS2 Insulin receptor substrate...Phosphatase and tensin homolog RAC RAC1 RAC2 RAC3 Ras-related C3 botulinum toxin substrate (rho family, small...v-ral simian leukemia viral oncogene homolog (ras related) RALBP1 RalA binding protein 1 RALGDS Ral guanine...

  16. COVID-19 Resources

    Type
    Collection
    ...the knowledge and understanding of COVID-19 and related coronaviruses. To assist with this effort Addgene...plasmids available that may be useful for COVID-19 related research. Additionally, we have linked to collections...articles, protocols, and other resource collections related to COVID-19 that may be of use to scientists. Background...entry of SARS-CoV and SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry... in the body. For a full list of cytokines and related plasmids see our Immunology Resource Guide . Return...Simple, portable, and inexpensive platforms to reliably detect nucleic acids at the atomolar level. For...publishers have allowed free access to articles related to SARS-CoV-2 research during the current outbreak...

  17. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ...estrogen-related receptor alpha ESRRB Plasmids ESRRB, DFNB35, ERR2, ERRb, ESRL2, NR3B2 estrogen-related receptor...Engineering Guide Empty-Backbone Resource Image is released to public domain by author. Source: Wikipedia,...Plasmids ESRRG, ERR3, ERRgamma, NR3B3 estrogen-related receptor gamma HNF4A Plasmids HNF4A, RP5-1013A22.1...MGC119326, MGC119329, RZR-ALPHA, DKFZp686M2414 RAR-related orphan receptor A RORB Plasmids RORB, NR1F2, ROR-BETA...ROR-BETA, RZR-BETA, RZRB, bA133M9.1 RAR-related orphan receptor B RORC Plasmids RORC, RP11-98D18.11-001...001, NR1F3, RORG, RZR-GAMMA, RZRG, TOR RAR-related orphan receptor C RXRA Plasmids RXRA, NR2B1 retinoid...

  18. Cre-lox system

    Type
    Collection
    ...Mammalian, Zebrafish Eisenhoffer 131782 pME-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN...Tol2 kit) Zebrafish Eisenhoffer 131783 pTol2-CreLite CreLite; Tol2 destination vector with mTagBFP2 Zebrafish...Zebrafish Eisenhoffer 131785 pAAV-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV ...transfer vector Cbh AAV Eisenhoffer 131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN...2A-Cre CAG Mammalian Breunig 131780 pCS2+PIF6CreN CreLite - Red light-inducible Cre; N terminus Cre fused...Mammalian, Zebrafish Eisenhoffer 131781 pCS2+PhyBΔCreC CreLite - Red light-inducible Cre; C terminus Cre fused...containing FLEx technology. Read the genOway press release for more information. Empty backbones to regulate...

  19. Genetic Code Expansion

    Type
    Collection
    ...coli azi-donorleucine (Anl) Bacterial ATG David Tirrell 62598 pKPY514 phenylalanyl-tRNA synthetase subunit...p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA synthetase (CePheRS...p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase (L274GMmMetRS...musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli...musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 91705 pSupAR-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers...using genetic code expansion without buildup of prematurely truncated protein. Ryan Mehl 197656 BL21(DE3)... by tolC, and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair ...

  20. mTOR Pathway

    Type
    Collection
    ...signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many cancers....Enhanced signaling through either mTORC1 or mTORC2 correlates with poor prognosis in many cancers, including...for clarity and does not indicate a specific relationship. The content for this page was generated with...tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related GTP binding Raptor Also known as RPTOR; Regulatory...

  21. p53 Pathway

    Type
    Collection
    ...Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase CASP9...for clarity and does not indicate a specific relationship. p53 Pathway Color is used for clarity and does... does not indicate a specific relationship. The content for this page was generated with the help of .... CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, 2, or 3 Cyclin...

  22. Synthetic Biology - Overview

    Type
    Collection
    ...Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including ...and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components....networks. Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing...

  23. Worm Expression Resources

    Type
    Collection
    ...nematodes, including C. elegans and C. briggsae. Other related miniMos plasmids can be found on the Jorgensen ...genetics, genomics and biology of C. elegans and related nematodes. WormBook - A comprehensive, open-access...original, peer-reviewed chapters covering topics related to the biology of C. elegans and other nematodes...

  24. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...sensor (MARIO) A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2. bioRxiv...Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Proc Natl Acad...sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin...-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(...

  25. Neurodegeneration Research Collection

    Type
    Collection
    ...common disorders followed by a list of some of the relevant collections available at Addgene. Amyotrophic ...growing collection of general and neuroscience -related antibodies including: Anti-KChIP1 K+ channel [K55...diagnostics and treatments for Alzheimer’s disease and related disorders. The site curates information on useful...

  26. CRISPR Guide

    Type
    Collection
    ...insert donor DNA at “landing sites” defined by relatively short sequences. Some have been paired with CRISPR...high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template to accurately... transduced cell population, you will look for relevant hits using NGS technology. For more detail on ...As this reporter is degraded by Cas12a, it will release a quantifiable signal that indicates the presence... J., & Van Der Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively...

  27. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-1-20297P 20297-AAV1 pAAV-EF1a-double ...20071P 20071-AAV9 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-9-20297P 20297-AAV9 pAAV-EF1a-double ...Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda AV-5-20949P 20949-AAV5 pAAV-double floxed-eNpHR-EYFP-WPRE-pA...

  28. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Addgene's collection of synthetic biology plasmids related to Networks and Gene Regulation...Addgene's collection of synthetic biology plasmids related to Networks and Regulation. The collection includes...

  29. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ... therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral proteins as a first step for...articles, protocols, and other resource collections related to COVID-19 that may be of use to scientists. Addgene's...

  30. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...dimensional structures of proteins of medical relevance, and place them in the public domain without restriction...TEV cleavage, Bac-to-Bac Expression System, AmpR Related Resources SGC: http://www.thesgc.org/ SGC Toronto...

  31. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...support educators interested in integrating MDH-related research into their teaching laboratories. They... plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression, structure ...

  32. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency...the reverse transcriptase. Many PE enzymes and related tools are available: PE2 - introduction of five...

  33. AAVED

    Type
    Collection
    ...Thursday, September 20 6:00 PM-8:00 PM Group Dinner Relax and Connect with Fellow Speakers and Attendees Friday...you will have final approval before anything is released publicly, but please only share materials that...

  34. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related protein LC3 B Autophagosomes 101784 SLC25A17-mEGFP...junctions 107579 RAB5A-mEGFP AICSDP-39 mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-...

  35. Plant Plasmids and Resources

    Type
    Collection
    ...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (..., computational and experimental resources that relate to the control of gene expression in the grasses...

  36. Zebrafish Plasmid Collection

    Type
    Collection
    ...lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, or labeling... previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled...

  37. Deisseroth INTRSECT Collection

    Type
    Collection
    ... NOT Cre No Dual recombinase-dependent: Rabies-related Addgene ID Plasmid Logic Sites and Mutations In-Stock... of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs...

  38. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...years later, PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve...developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly...

  39. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Human Mouse IgG2a 114490 Anti-Kirrel3, short and long [N321C/49R] Kirrel3, short and long Rat Mouse IgG2a... Rat Mouse IgG2a 149452 Anti-Kirrel3, short isoform 3 [N320/48R] Kirrel3, short isoform 3 Mouse Mouse ...

  40. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV ALS Alicia Pickrell 131792 pHAGE-TBK1-C-FLAG-HA TBK1 STOP codon CMV ALS Alicia Pickrell 131889 pDONR221...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic Lateral...pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ALS Trina ...

  42. Synthetic Biology - Strains

    Type
    Collection
    ...Browse Addgene's collection of synthetic biology related bacterial strains. SynBio Bacterial Strains Search...

  44. Neuroscience

    Type
    Collection
    ... recombinant antibodies targeting neuroscience-related proteins....

  45. Synthetic Biology - Metabolism

    Type
    Collection
    ...Addgene's collection of synthetic biology plasmids related to metabolic pathways and components. Examples ...

  47. Zinc Finger Consortium Reagents

    Type
    Collection
    ...for expressing zinc finger FokI nuclease fusions Related Genome Engineering Technologies TALEN Engineering...

  50. NETRF

    Type
    Collection
    ...effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that...
Showing: 1 - 50 of 60 results