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Showing: 181 - 200 of 117101 results

  1. HDM-SARS2-Spike-delta21

    Plasmid
    #155130
    Purpose
    Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping
    Depositor
    Jesse Bloom
    Publication
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Insert
    SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon optimized to H. sapiens using IDT codon opt…
    Promoter
    CMV
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20 Articles
    155130-plasmid-map-sequence-id-306280

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  3. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55 Articles
    61357-plasmid-map-sequence-id-358013

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  4. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Publication
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    31 Articles
    60847-plasmid-map-sequence-id-197511

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  9. HDM-SARS2-Spike-delta21

    Plasmid
    #155130
    Purpose
    Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping
    Depositor
    Jesse Bloom
    Publication
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Insert
    SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon optimized to H. sapiens using IDT codon opt…
    Promoter
    CMV
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20 Articles
    155130-plasmid-map-sequence-id-306280

    Enlarge

  10. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55 Articles
    61357-plasmid-map-sequence-id-358013

    Enlarge

  13. pBOB-EF1-FastFUCCI-Puro

    Plasmid
    #86849
    Purpose
    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection
    Depositor
    Kevin Brindle, Duncan Jodrell
    Publication
    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164
    Inserts
    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1 and PGK
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46 Articles
    86849-plasmid-map-sequence-id-237968

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  14. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    61358-plasmid-map-sequence-id-99789

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  17. pBOB-CAG-SARS-CoV2-Spike D614G-HA

    Plasmid
    #158761
    Purpose
    3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Publication
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Spike D614G
    Promoter
    CAG
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    158761-plasmid-map-sequence-id-309514

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  18. pBOB-EF1-FastFUCCI-Puro

    Plasmid
    #86849
    Purpose
    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection
    Depositor
    Kevin Brindle, Duncan Jodrell
    Publication
    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164
    Inserts
    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1 and PGK
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46 Articles
    86849-plasmid-map-sequence-id-237968

    Enlarge

Showing: 181 - 200 of 117101 results