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pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*GFP

Plasmid

#162806

Purpose

STELLA plasmid for amber suppression of N-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

EF1_Ub-(N-TAG)GFP

Use

Piggybac

Expression

Mammalian

Mutation

GFP (Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a-CylA

Plasmid

#48198

Purpose

Expresses CylA (fatty acyl-AMP ligase) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylA

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-IQ

Plasmid

#61678

Purpose

GFP-labeled SAH scaffold with IQ attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-IQ

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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Xlone-tTA-42xRCGG/CGA/AGG

Plasmid

#245728

Purpose

Express 42xRCGG/CGA/AGG mRNA in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

42xRCGG/CGA/AGG

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDWJ16

Plasmid

#207599

Purpose

GFP-BAA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BAA

Expression

Bacterial

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ22

Plasmid

#207602

Purpose

GFP-AABA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AABA

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ18

Plasmid

#207601

Purpose

GFP-ABA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-ABA

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ17

Plasmid

#207600

Purpose

GFP-ABB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-ABB

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ15

Plasmid

#207598

Purpose

GFP-BBA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BBA

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ14

Plasmid

#207597

Purpose

GFP-AABB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AABB

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ13

Plasmid

#207596

Purpose

GFP-AAB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AAB

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJMG2

Plasmid

#207595

Purpose

GFP-BAB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BAB

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJS109

Plasmid

#207590

Purpose

GFP-ABBA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-ABBA

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2

Plasmid

#191320

Purpose

Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS1272

Plasmid

#188891

Purpose

Ric-4 sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(R07E5.16)::Ric-4 sgRNA (CELE_Y22F5A.3 Nematode)

Expression

Worm

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS715

Plasmid

#188887

Purpose

Multisite Gateway [4-3] Destination vector; lox2272-flanked Cbr-unc-119 marker; homology to 5' miniMos Arm

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Type

Empty backbone

Use

Multisite gateway [4-3] destination vector

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S10

Plasmid

#128594

Purpose

Expresses tagless S10 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S10 (rpsJ E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S3

Plasmid

#128591

Purpose

Expresses tagless S3 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S3 E.coli ribosomal protein (rpsC E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

Plasmid

#162803

Purpose

Control STELLA destination plasmid for N-terminally labelled proteins

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub(G75,76A)-BsmBI

Use

Piggybac

Expression

Mammalian

Mutation

Ubiquitin (G75,76A)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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