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We narrowed to 23,564 results for: CHI

Showing: 5401 - 5450 of 23564 results

  2. pBabe 3XFLAG-T34A-GATA6-3XAU1 puro

    Plasmid
    #72609
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutation
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed threonine34 to alanine
    Promoter
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. pBabe 3XFLAG-S33A-GATA6-3XAU1 puro

    Plasmid
    #72610
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutation
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed serine33 to alanine
    Promoter
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. pEN_TT 3XFLAG-S37A-GATA6-3XAU1

    Plasmid
    #72613
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Entry vector for gateway cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Mutation
    Changed serine37 to alanine
    Promoter
    TRE tight promoter
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72613-plasmid-map-sequence-id-138777

    Enlarge

  5. pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

    Plasmid
    #72611
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed serine33, threonine34 and serine37 to ala…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV-FLEX-DTR-GFP (AAV2)

    Viral Prep
    #124364-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-FLEX-DTR-GFP (#124364). In addition to the viral particles, you will also receive purified pAAV-FLEX-DTR-GFP plasmid DNA. CBA-driven, Cre-dependent expression of DTR-GFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Eiman Azim, Thomas Jessell
    Article
    Azim et al Nature. 2014 Apr 17;508(7496):357-63. do
    Promoter
    Chicken B-actin
    Tags
    GFP (Cre-dependent)
    Available since
    June 16, 2020
    Availability
    Academic Institutions and Nonprofits only

  11. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid
    #50486
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    GFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the GFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pCDNA3.1-nAChr-beta3-YFP

    Plasmid
    #50484
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    YFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherry

    Plasmid
    #172637
    Purpose
    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cells
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Insert
    CCND1 (CCND1 Human)
    Use
    Lentiviral
    Tags
    2xFLAG-2xSTREP
    Expression
    Mammalian
    Mutation
    Pro287Ala
    Promoter
    CMV
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172637-plasmid-map-sequence-id-340160

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  21. pET28a_DmKHC[1-421]-SNAP-6xHis

    Plasmid
    #196975
    Purpose
    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag
    Depositor
    Lukas Kapitein
    Article
    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    6xHis and SNAP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196975-plasmid-map-sequence-id-388920

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  29. pLVX-2xFLAG-2xSTREP-CCND1(T286A)-IRES-mCherry

    Plasmid
    #172639
    Purpose
    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and mCherry in mammalian cells
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Insert
    CCND1 (CCND1 Human)
    Use
    Lentiviral
    Tags
    2xFLAG-2xSTREP
    Expression
    Mammalian
    Mutation
    Thr286Ala
    Promoter
    CMV
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172639-plasmid-map-sequence-id-340169

    Enlarge

  32. pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201820
    Purpose
    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging
    Depositor
    Christopher Richie
    Article
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    SYN1
    Available since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201820-plasmid-map-sequence-id-399615

    Enlarge

  35. pLVX-2xFLAG-2xSTREP-CCND1(P287S)-IRES-mCherry

    Plasmid
    #172635
    Purpose
    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287S) and mCherry in mammalian cells
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Insert
    CCND1 (CCND1 Human)
    Use
    Lentiviral
    Tags
    2xFLAG-2xSTREP
    Expression
    Mammalian
    Mutation
    Pro287Ser
    Promoter
    CMV
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172635-plasmid-map-sequence-id-340162

    Enlarge

  40. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid
    #136659
    Purpose
    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136659-plasmid-map-sequence-id-273359

    Enlarge

  42. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201821
    Purpose
    An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET
    Depositor
    Christopher Richie
    Article
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    SYN1
    Available since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201821-plasmid-map-sequence-id-399618

    Enlarge

  45. pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201822
    Purpose
    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging
    Depositor
    Christopher Richie
    Article
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CaMKii
    Available since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201822-plasmid-map-sequence-id-399617

    Enlarge

  46. pCAGGS-SMSr-TEV-Twin-Strep

    Plasmid
    #202530
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SAMD8 (SAMD8 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TGA)
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202530-plasmid-map-sequence-id-406370

    Enlarge

  47. pInducer 21-3xFlag-hMEFV-F479L

    Plasmid
    #195477
    Purpose
    pInducer21 plasmid containing the human MEFV gene with the F479L mutation (associated with Familial Mediterranean Fever) with a 3xFlag tag at the N-terminus
    Depositor
    Thomas Henry
    Article
    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d
    Insert
    MEFV (MEFV Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed phenylalanine 479 to leucine
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    195477-plasmid-map-sequence-id-386697

    Enlarge

  48. pInducer 21-3xFlag-hMEFV-ΔPYD

    Plasmid
    #195478
    Purpose
    pInducer21 plasmid containing the human MEFV gene with a deletion of residues 1 - 92 (the resulting pyrin protein lacks the PYD domain) with a 3xFlag tag at the N-terminus
    Depositor
    Thomas Henry
    Article
    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d
    Insert
    MEFV (MEFV Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    deletion of residues 1-92 of pyrin (the PYD domai…
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    195478-plasmid-map-sequence-id-386693

    Enlarge

  49. pInducer21-3xFlag-hMEFV-Q426R

    Plasmid
    #195475
    Purpose
    pInducer21 plasmid containing the human MEFV gene with the Q426R mutation (associated with Familial Mediterranean Fever) with a 3xFlag tag at the N-terminus
    Depositor
    Thomas Henry
    Article
    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d
    Insert
    MEFV (MEFV Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed glutamine 426 to arginine
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    195475-plasmid-map-sequence-id-386689

    Enlarge

  50. pInducer 21-3xFlag-hMEFV-S580X

    Plasmid
    #195474
    Purpose
    pInducer21 plasmid containing the human MEFV gene with the S580X mutation (stop codon leading to the truncation of the B30.2 domain of the pyrin protein) with a 3xFlag tag at the N-terminus
    Depositor
    Thomas Henry
    Article
    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d
    Insert
    MEFV (MEFV Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed S580 to a stop codon
    Promoter
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    195474-plasmid-map-sequence-id-386691

    Enlarge

Showing: 5401 - 5450 of 23564 results