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We narrowed to 16,166 results for: grna

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Showing: 241 - 260 of 16166 results

  3. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42333
    Purpose
    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    March 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    42333-plasmid-map-sequence-id-59273

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  4. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)

    Viral prep
    #92379-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .
    Depositor
    John Doench, David Root
    Publication
    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:
    Available since
    Aug. 3, 2017
    Availability
    Academic Institutions and Nonprofits only

  6. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Publication
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Promoter
    Tight TRE promoter
    Available since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    82 Articles
    87360-plasmid-map-sequence-id-473508

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  7. Multiplex CRISPR/Cas9 Assembly System Kit

    Plasmid kit
    #1000000055
    Purpose
    Plasmids to create all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs for genome engineering using Cas9 nuclease or Cas9 D10A nickase.
    Depositor
    Takashi Yamamoto
    Application
    Genome Editing
    Vector type
    Mammalian Expression
    Editing type
    CRISPR
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  10. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Publication
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    140096-plasmid-map-sequence-id-292016

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  19. CRISPRainbow Kit for Multiplex Labeling

    Plasmid kit
    #1000000079
    Purpose
    Plasmids using dCas9 and gRNA scaffolds that bind fluorescent proteins to label multiple chromosomal loci.
    Depositor
    Thoru Pederson
    Application
    Genome Editing
    Vector type
    Lentiviral, Mammalian Expression
    Editing type
    CRISPR
    Available since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 260 of 16166 results