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176,133 results

Showing: 6451 - 6500 of 176133 results

  1. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  9. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid
    #196196
    Purpose
    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus
    Depositor
    Jürgen Knoblich
    Article
    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75
    Insert
    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    Use
    Homology repair donor
    Tags
    V5-APEX2-mScarlet-I
    Available Since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    196196-plasmid-map-sequence-id-385065

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  10. TFORF3325

    Plasmid
    #144801
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DLX3 (DLX3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144801-plasmid-map-sequence-id-391551

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  12. pAAV-EF1a-Flp-DOG-NW (AAV1)

    Viral Prep
    #75469-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-Flp-DOG-NW (#75469). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flp-DOG-NW plasmid DNA. EF1a-driven expression of Flp recombinase dependent on GFP (Flp-DOG); to be coinjected with GFP (or for use with transgenic GFP animals). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Connie Cepko
    Article
    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:
    Promoter
    EF-1a
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only

  21. HaloTag-STAG1 Vector

    Plasmid
    #238689
    Purpose
    Express HaloTag-STAG1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    STAG1 (STAG1 Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238689-plasmid-map-sequence-id-479895

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  22. pAAV-CaMKIIa-EGFP (AAV1)

    Viral Prep
    #50469-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    EGFP
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  27. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

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  28. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid
    #172603
    Purpose
    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    The multiple cloning site (MCS) has been edited/e…
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172603-plasmid-map-sequence-id-340216

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  29. DsRed2-pBAD

    Plasmid
    #54608
    Purpose
    Localization: Protein Expression Vector , Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and DsRed2
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54608-plasmid-map-sequence-id-162860

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  30. pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag (AAV5)

    Viral Prep
    #209655-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag (#209655). In addition to the viral particles, you will also receive purified pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag plasmid DNA. CAG-driven, Cre-dependent expression of the high affinity ATP biosensor iATPSnFR2. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Article
    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12
    Promoter
    CAG
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  37. pMN-332

    Plasmid
    #195466
    Purpose
    Lentiviral Integratable Inducible synZiFTR ZF1 + Reporter
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    inverted[8X ZF1 BS - ybTATA - mCherry] - pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195466-plasmid-map-sequence-id-384256

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  39. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV8)

    Viral Prep
    #105545-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    HI
    Tags
    EGFP
    Available Since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  40. pCWXPGR-pTF-Kir2.1

    Plasmid
    #114284
    Purpose
    TET-inducible expression of the Kir2.1 protein
    Depositor
    Patrick Salmon
    Insert
    Kir2.1 (KCNJ2 Human)
    Use
    Lentiviral
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114284-plasmid-map-sequence-id-218907

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  45. pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

    Plasmid
    #203349
    Purpose
    Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    mGreenLantern
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Promoter
    T7
    Available Since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203349-plasmid-map-sequence-id-399147

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Showing: 6451 - 6500 of 176133 results