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173,387 results

Showing: 5401 - 5440 of 173387 results

  3. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100087-plasmid-map-sequence-id-197556

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  11. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177433
    Purpose
    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177433-plasmid-map-sequence-id-348464

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  13. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV1)

    Viral Prep
    #105530-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    EGFP
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. TFORF2631

    Plasmid
    #144140
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    INSM1 (INSM1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144140-plasmid-map-sequence-id-392338

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  21. CD44I-c001

    Plasmid
    #175448
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175448-plasmid-map-sequence-id-353140

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  22. pT7-V5-SBP-C1-HshnRNPE2iso3_Z

    Plasmid
    #148140
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Available Since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148140-plasmid-map-sequence-id-385303

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  25. PB-TO-MYOD1-shOct4

    Plasmid
    #182309
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    shOct4
    Promoter
    EF1a and TRE3G
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182309-plasmid-map-sequence-id-359523

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  31. DEXsynth:ccdb-3xHA + mcherry

    Plasmid
    #233314
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and mCherry marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:ccdb-3xHA + mcherry
    Use
    Gateway destination
    Tags
    3xHA
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    233314-plasmid-map-sequence-id-462184

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  32. Galt1-HT7-mGold

    Plasmid
    #230027
    Purpose
    Expressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-HaloTag7-mGold (B4GALT1 Human, Synthetic)
    Tags
    HaloTag,mGold
    Expression
    Mammalian
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230027-plasmid-map-sequence-id-458169

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  38. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)

    Viral Prep
    #154867-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Article
    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available Since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  39. pZFN-AAVS1-KKR

    Plasmid
    #159298
    Purpose
    ZFN (right) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Kosuke Yusa
    Insert
    ZFN targeting the AAVS1 locus
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    159298-plasmid-map-sequence-id-311594

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Showing: 5401 - 5440 of 173387 results