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pCI-neo beta catenin S33Y

Plasmid

#16519

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

beta catenin (CTNNB1 Human)

Expression

Mammalian

Mutation

S33Y (SW48 mutation)

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pCI-neo beta catenin S33Y

Plasmid

#16519

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

beta catenin (CTNNB1 Human)

Expression

Mammalian

Mutation

S33Y (SW48 mutation)

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pHCMV-LCMV-WE

Plasmid

#15793

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Lymphocytic choriomeningitis virus glycoprotein LCMVGP gene

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pHCMV-LCMV-WE

Plasmid

#15793

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Lymphocytic choriomeningitis virus glycoprotein LCMVGP gene

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pMax-E2F1

Plasmid

#16007

Depositor

Olimpia Meucci

Article

Shimizu et al Neurobiol Dis. 2007 Jan . 25(1):17-26.

Insert

E2F1 (E2F1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pMax-E2F1

Plasmid

#16007

Depositor

Olimpia Meucci

Article

Shimizu et al Neurobiol Dis. 2007 Jan . 25(1):17-26.

Insert

E2F1 (E2F1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pCGN-ATF6

Plasmid

#11974

Purpose

For mammalian expression of human ATF6 with an HA tag

Depositor

Ron Prywes

Article

Zhu et al Mol Cell Biol. 1997 Sep . 17(9):4957-66.

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pCGN-ATF6

Plasmid

#11974

Purpose

For mammalian expression of human ATF6 with an HA tag

Depositor

Ron Prywes

Article

Zhu et al Mol Cell Biol. 1997 Sep . 17(9):4957-66.

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
V245 pCEP-4HA B56alpha

Plasmid

#14532

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)

Tags

4 HA

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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V245 pCEP-4HA B56alpha

Plasmid

#14532

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)

Tags

4 HA

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC myc hPOT1

Plasmid

#12387

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 (POT1 Human)

Use

Retroviral

Tags

Myc

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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pLPC myc hPOT1

Plasmid

#12387

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 (POT1 Human)

Use

Retroviral

Tags

Myc

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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RSV-Sp1

Plasmid

#12098

Depositor

Robert Tjian

Insert

Sp1 (SP1 Human)

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
RSV-Sp1

Plasmid

#12098

Depositor

Robert Tjian

Insert

Sp1 (SP1 Human)

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDx_mScarlet-I3

Plasmid

#189757

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDx_mScarlet-I3

Plasmid

#189757

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Expression

Bacterial

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Expression

Bacterial

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP-HaloTag7-LC3

Plasmid

#184899

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP-HaloTag7-LC3

Plasmid

#184899

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-MMLVgag-3xNES-ABE8e-NG

Plasmid

#181754

Purpose

MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE8e-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-MMLVgag-3xNES-ABE8e-NG

Plasmid

#181754

Purpose

MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE8e-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Article

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Tags

luciferase

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Article

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Tags

luciferase

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-GRAB_DA1h (AAV9)

Viral Prep

#113050-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_DA1h (#113050). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_DA1h plasmid DNA. Synapsin-driven expression of GRAB-DA1h dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Yulong Li

Article

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Promoter

Syn

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only
pET-32a(+)-TGFB3

Plasmid

#120288

Purpose

Produce human recombinant TGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-32a(+)-TGFB3

Plasmid

#120288

Purpose

Produce human recombinant TGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_tre_dCas9_KRAB

Plasmid

#126030

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with KRAB
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_tre_dCas9_KRAB

Plasmid

#126030

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with KRAB
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-Flag-c-Myc

Plasmid

#102626

Purpose

Expresses c-Myc in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

MYC proto-oncogene, bHLH transcription factor (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-Flag-c-Myc

Plasmid

#102626

Purpose

Expresses c-Myc in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

MYC proto-oncogene, bHLH transcription factor (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW12.lentiguide.GFP

Plasmid

#104374

Purpose

GFP vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW12.lentiguide.GFP

Plasmid

#104374

Purpose

GFP vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG mito-GCaMP5G

Plasmid

#105009

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

GCaMP5G

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG mito-GCaMP5G

Plasmid

#105009

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

GCaMP5G

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Antares2 c-myc

Plasmid

#100027

Purpose

A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

Antares2

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Antares2 c-myc

Plasmid

#100027

Purpose

A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

Antares2

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGEN-CMV-CjCas9

Plasmid

#89752

Purpose

Expression of CjCas9 in mammalian cells

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

Cas9

Use

CRISPR

Tags

SV40-HA

Expression

Mammalian

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGEN-CMV-CjCas9

Plasmid

#89752

Purpose

Expression of CjCas9 in mammalian cells

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

Cas9

Use

CRISPR

Tags

SV40-HA

Expression

Mammalian

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCB320

Plasmid

#89359

Purpose

3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

puromycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCB320

Plasmid

#89359

Purpose

3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

puromycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TRE-KRAB-dCas9-IRES-BFP

Plasmid

#85449

Purpose

Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-BFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TRE-KRAB-dCas9-IRES-BFP

Plasmid

#85449

Purpose

Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-BFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD

Expression

Mammalian

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD

Expression

Mammalian

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
dCAS9-VP64_GFP (Concentrated Lentiviral Prep)

Viral Prep

#61422-LVC

Purpose

Ready-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Promoter

EF1a

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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