We narrowed to 19,393 results for: IRE

Showing: 7551 - 7600 of 19393 results

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pMSCVhyg-3xT7-nSREBP1a

Plasmid

#171030

Purpose

Expresses 3xT7-tagged nuclear form of SREBP1a in mammalian cells

Depositor

Kai Ge

Article

Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

Insert

nSREBP1a (Srebf1 Mouse)

Use

Retroviral

Tags

3xT7

Expression

Mammalian

Mutation

amino acids 1- 449

Promoter

5' LTR

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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myc-TRX

Plasmid

#21614

Depositor

John Kyriakis

Article

Liu et al Mol Cell Biol. 2000 Mar . 20(6):2198-208

Insert

thioredoxin (TXN Human)

Tags

Myc

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
PX552-mScn2a-3xV5-EF1-smFP-flag

Plasmid

#182561

Purpose

For mouse Nav1.2 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn2a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGH153_MS2-AIDΔ-Hygro

Plasmid

#85416

Purpose

lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AIDΔ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-LAMP1-mRFP

Plasmid

#169004

Purpose

Express rat LAMP1-mRFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

Insert

rat LAMP1-mRFP

Use

Unspecified

Tags

mRFP

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB1691-Nluc

Plasmid

#204324

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

KB1691-Nluc

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-flag-GFP-MUC4

Plasmid

#205189

Purpose

This vector encodes of a synCAM (MUC4) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM MUC4 and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Hnf4a

Plasmid

#33002

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

hepatic nuclear factor 4 alpha (Hnf4a Mouse)

Use

Retroviral

Available Since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro

Plasmid

#211622

Purpose

Rhno1 S141A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S141A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD+Red-F

Plasmid

#170874

Purpose

Phytobrick - Red-F Luciferase CDS

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

Red-F

Use

Luciferase and Synthetic Biology

Mutation

p.S286Y Red mutant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

Plasmid

#202025

Purpose

Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

Depositor

Mikhail Shapiro

Article

Hurt et al bioRxiv 2024.03.30.587094

Insert

Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)

Expression

Bacterial

Mutation

GvpB S9G-R31L-R85L

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-ZnT1-WT

Plasmid

#82340

Purpose

Expresses FLAG-tagged ZnT1-WT in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

ZnT1-WT (SLC30A1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGrDL_SPb

Plasmid

#83205

Purpose

receives miRNA target sequences for testing using the dual luciferase assay system

Depositor

Peer Schenk

Article

Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

Insert

Tomato ACTIN promoter

Expression

Plant

Promoter

Tomato ACTIN

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_mCherry

Plasmid

#26823

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-mCherry-3'UTR

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
pMM113

Plasmid

#127210

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, AtSTM, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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acta1-mRuby2-dGBP

Plasmid

#163855

Purpose

Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

dVHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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sgTrack-GFP

Plasmid

#114012

Purpose

Empty sgRNA expression vector with co-expression of TurboGFP reporter

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn-FLEX.iGABASnFR

Plasmid

#112163

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR

Use

AAV

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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BII-CPspaH2B

Plasmid

#133385

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTECH-MmAcK3RS(IPYE)

Plasmid

#104071

Purpose

Expression plasmid encoding MmAcK3RS(IPYE) and pylT

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp MmAcK3RS(IPYE); PproK pyl

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-mTOR (1-1482 aa)

Plasmid

#21745

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1-1482) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1-1482

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2-scr

Plasmid

#169795

Purpose

Expresses Cas9 and guide RNA with untarget sequence generated by scramble of the target sequence of LentiCRISPRv2-ACTB-C1..

Depositor

Natsuko Chiba

Article

Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

Insert

Scrambled guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-TfR-RUSH

Plasmid

#166905

Purpose

RUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-Halo

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCB6-HFE-EGFP

Plasmid

#12104

Depositor

Pamela Bjorkman

Article

Giannetti et al J Biol Chem. 2004 Jun 11. 279(24):25866-

Insert

HFE (HFE Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-Ran-Q69L-mRFP1-polyA

Plasmid

#104560

Purpose

Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Tags

mRFP1

Expression

Mammalian

Mutation

Q69L

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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OSCA1.2

Plasmid

#136593

Purpose

Expresses the mechanosensitive channel OSCA1.2 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA1.2 (AT4G22120 Mustard Weed)

Tags

pIRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X dAmp/Kana+

Plasmid

#154352

Purpose

Empty backbone vector

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Type

Empty backbone

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRN119

Plasmid

#176884

Purpose

Encodes STM and WUS2 for ectopic meristem induction in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S

Expression

Plant

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-p18-CIBN

Plasmid

#188990

Purpose

A lentiviral plasmid encoding lysosome-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Tags

p18

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FsC_pET29b

Plasmid

#215405

Purpose

Expression construct for FsC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

FsC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAYP (pDre)

Plasmid

#62709

Purpose

Mammalian expression of Dre recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

Dre

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRXIP-GFP-YKT6

Plasmid

#116946

Purpose

Expressing GFP-YKT6 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

Synaptobrevin homolog YKT6 (YKT6 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-GBP-MTn

Plasmid

#154363

Purpose

Bacterial expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

His

Expression

Bacterial

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM146

Plasmid

#127218

Purpose

BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, BBM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HIR K1030R

Plasmid

#24050

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

K1030R

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-cyto-KB1691-Nluc

Plasmid

#204326

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

cytosolic KB1691-Nluc

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
BiP 2: C. elegans BiP (hsp-4)-GFP

Plasmid

#21896

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

BiP (hsp-4 Nematode)

Tags

GFP

Expression

Worm

Mutation

Fusion between the GFP and hsp-4 takes place at A…

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
piggybac-EF1-halo-Oct4

Plasmid

#197909

Purpose

single-particle tracking for OCT4

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

pou5f1 (Pou5f1 Mouse)

Tags

Halotag

Expression

Mammalian

Promoter

EF1

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ThcCut1_pET29b

Plasmid

#215429

Purpose

Expression construct for ThcCut1 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

ThcCut1

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
acta1-mRuby2-VHH[GFP]

Plasmid

#163856

Purpose

Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

VHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3TS-CD44b-TurboID

Plasmid

#163850

Purpose

In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

CD44b (cd44b Zebrafish)

Use

In vitro transcription of rna

Tags

TurboID

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAT11

Plasmid

#204290

Purpose

For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-DsRed

Use

CRISPR

Promoter

ie1

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-RosaR4 KKR mutations

Plasmid

#37199

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Insert

FokI (fused to RosaR4 ZFN)

Use

Zfn expression

Tags

HA, ROSA26 eZFNs R4, and SV40 NLS

Expression

Mammalian

Mutation

I538K, E490K, and H537R

Promoter

CMV

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM135

Plasmid

#127216

Purpose

BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, WUS2, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_mouse-pro-IL1B

Plasmid

#183386

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL1B (Il1b Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGs-Foxp1

Plasmid

#45720

Depositor

Jeremy Dasen

Article

Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

Insert

FoxP1 (Foxp1 Mouse)

Promoter

CMV / b-actin

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
pKanB alpha

Plasmid

#223524

Purpose

Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Article

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Expression

Yeast

Promoter

AOX1

Available Since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA-RanWT-mRFP1-polyA

Plasmid

#59750

Purpose

Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran-mRFP1

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM136

Plasmid

#127217

Purpose

BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, MP-delta, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mito-Citroff1-S245

Plasmid

#134306

Purpose

mitochondria-targeted inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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