We narrowed to 16,909 results for: igh

1. pNIC-AncCAR_A

   Plasmid

   #168286

   Purpose

   Expresses AncCAR_A in E. coli from a T7 promoter

   Depositor

   Nicholas Harmer

   Article

   Thomas et al Commun Biol. 2019 Nov 22;2:429. doi: 10.

   Insert

   AncCAR_A
   

   Use

   Synthetic Biology

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Amph1-pmCherryN1

   Plasmid

   #27692

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Amphiphysin 1 (Amph Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MXS_CreERT2

   Plasmid

   #62427

   Purpose

   MXS_chaining vector with CreERT2

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   Tamoxifen inducible Cre recombinase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSC101-GFPmut2-mScarlet-I

   Plasmid

   #208183

   Purpose

   mistranslation GFP and mScarlet-I positive control

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCHW110031

   Plasmid

   #222437

   Purpose

   Level 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. N1-G-Ca-FLITS

   Plasmid

   #191465

   Purpose

   Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mOrange2-Lysosomes-20

   Plasmid

   #57968

   Purpose

   Localization: Lysosome Membrane, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   LAMP1
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSW036

   Plasmid

   #140034

   Purpose

   KO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoter

   Depositor

   Peter Nixon

   Article

   Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

   Insert

   Yellow fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cpt

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTTP1B

   Plasmid

   #91723

   Purpose

   integration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNA

   Depositor

   Graham Hatfull

   Article

   Pham et al Microbiology. 2007 Aug;153(Pt 8):2711-23

   Type

   Empty backbone

   Use

   Integration-proficient plasmid vectors for mycoba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ134B2.0

    Plasmid

    #167158

    Purpose

    Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-EgI-HygroR

    Plasmid

    #207177

    Purpose

    Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-HygroR

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD/His-miRFP703

    Plasmid

    #79985

    Purpose

    Monomeric near-infrared fluorescent protein miRFP703 in bacterial plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    miRFP703
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pC28-LIC-sfGFP-His

    Plasmid

    #165479

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-GFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV Sport2 mPer1

    Plasmid

    #16203

    Depositor

    Cheng Lee

    Article

    Albrecht et al Cell. 1997 Dec 26. 91(7):1055-64.

    Insert

    Per1 (Per1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pHH0103_PCMT1_PCMT

    Plasmid

    #109932

    Purpose

    Protein expression and purification of PCMT1_PCMT

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PCMT1_PCMT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NM9-SpCas9-NLS3

    Plasmid

    #128177

    Purpose

    Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    SpCas9-NLS3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpCas9-CBE6a-IVT

    Plasmid

    #215833

    Purpose

    Template for in vitro transcription of CBE6a (with SpCas9)

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a-SpCas9-2xUGI
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAD/HisB-PAmKate

    Plasmid

    #32691

    Depositor

    Vladislav Verkhusha

    Article

    Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

    Insert

    PAmKate
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F84W/S148N/S165G compared to mKate (but numbering…

    Promoter

    araB

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFPL-Rh

    Plasmid

    #61649

    Purpose

    Gateway vector for generating red fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    monomeric red fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-R1R2-EM7neoPheS

    Plasmid

    #112923

    Purpose

    piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    neoPheS
    

    Use

    Piggybac transposon gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK5_tAKAR4α

    Plasmid

    #119919

    Purpose

    Mammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imaging

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKAR4α
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-Tre

    Plasmid

    #123134

    Purpose

    Mammalian expression vector for Tre.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Tre recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAYP (pDre)

    Plasmid

    #62709

    Purpose

    Mammalian expression of Dre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    Dre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.3_mCherry

    Plasmid

    #26823

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-mCherry-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

25. CMV-CitronRH

    Plasmid

    #158545

    Purpose

    Mammalian expression of nonbinding variant of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitronRH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Ca-FLARE (protease)

    Plasmid

    #92214

    Purpose

    protease component of FLARE

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    CaM-V5-TEVp(Δ220-242)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJ204-loxP-100RPU.2-ETN-lox2272

    Plasmid

    #154833

    Purpose

    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanercept

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    ETN
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    100RPU.2

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCZGY2850

    Plasmid

    #87758

    Purpose

    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pcol-19-PH-miniSOG(Q103L)
    

    Use

    Tags

    PH

    Expression

    Worm

    Mutation

    Q103L

    Promoter

    Pcol-19

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Human RNF144A (16-228)

    Plasmid

    #110752

    Purpose

    Bacterial Expression of RNF144A RBR domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    RNF144A (RNF144A Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSW071

    Plasmid

    #140037

    Purpose

    PCC 11901 fadD locus KO with a kanamycin resistance cassette

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Kanamycin resistance gene
    

    Use

    Gene knockout in cyanobacteria

    Tags

    Expression

    Mutation

    Promoter

    KanR

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKm-HCBE

    Plasmid

    #170333

    Purpose

    a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized CBEs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9n-D10A

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCHW100002

    Plasmid

    #222440

    Purpose

    Level 0, CDS module (AATG-GCTT) containing rice OsL5.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    OsL5 (Oryza sativa)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMK392 (mAID-mClover-BSD)

    Plasmid

    #121193

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mAID-mClover-BS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28_Caspase-4_K356D

    Plasmid

    #214312

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    K356D

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV10-3xFLAG-prs-XK

    Plasmid

    #184369

    Purpose

    Express human XK in Expi293 cells

    Depositor

    Karin Reinisch

    Article

    Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

    Insert

    Human XK (XK Human)
    

    Use

    Tags

    3xFLAG-PreScission cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GeNL(Ca2+)_520/pcDNA3

    Plasmid

    #85204

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_520
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKm-HGBE

    Plasmid

    #170334

    Purpose

    a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized GBEs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9n-D10A

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. YFP-DHFR-myc

    Plasmid

    #118852

    Purpose

    For fluorophore translocation assay and inducible tagging with zapalog.

    Depositor

    Thomas Schwarz

    Article

    Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

    Type

    Empty backbone

    Use

    Tags

    DHFR, Myc, and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKm-HABE

    Plasmid

    #170332

    Purpose

    a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized ABEs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9n-D10A

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5-H2B_Halo_T2A_EGFP

    Plasmid

    #135444

    Purpose

    Mamalian cell expression of nuclear HaloTag7 and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    H2B-HaloTag7 and EGFP
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge