169,450 results
-
Plasmid#11783DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only
-
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
Tags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-MEG3
Plasmid#44727DepositorAvailable SinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
ExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…Available SinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry (AAV Retrograde)
Viral Prep#114470-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherryAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon 300
Plasmid#163115PurposeRed fluorescent protein-based glucose sensor for mammalian cells with higer affinityDepositorInsertRed Glifon 300
ExpressionMammalianPromoterCMVAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
EB3-tdTomato
Plasmid#50708Purposeexpresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTsDepositorAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔABCF
Bacterial Strain#102270PurposeompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteinsDepositorBacterial ResistanceTetracyclineAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAD-sfGFP(Sp)-Pman
Plasmid#133833PurposeFluorescent protein sfGFP(Sp); under control of mannose inducible promoter.DepositorInsertsfGFP(Sp)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPmanAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK CA ROCK2
Plasmid#84649PurposeLentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of VenusDepositorInsertROCK2 (ROCK2 Bovine)
UseLentiviralExpressionMammalianMutationConstitutive Active (please see depositor comment…Available SinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralExpressionMammalianPromotermouse U6Available SinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
AiP12609 - pAAV-AiE0779m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2609)
Plasmid#191705PurposeAI plasmid ALIAS: AiP12609. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)DepositorInsertSYFP2
UseAAVMutationnoneAvailable SinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Str-Golgin84_VSVG-SBP-EGFP
Plasmid#65305Purposesynchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianPromoterCMVAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
N-Cadherin-EGFP
Plasmid#18870DepositorAvailable SinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
p(+)MV-NSE-FlagP-M502-3p
Plasmid#58748PurposeExpresses recombinant measles virus with Flag-P gene tagged si2 (siP) target sequenceDepositorInsertMeasles virus antigenome
TagsFlagPromoterT7Available SinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-DIO-tTA (AAV1)
Viral Prep#99121-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-ihSyn1-DIO-tTA (#99121). In addition to the viral particles, you will also receive purified pAAV-ihSyn1-DIO-tTA plasmid DNA. Cre-dependent expression of the tet-off transactivator from an inducible synapsin promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterihSyn1Available SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
SGEP
Plasmid#111170PurposemiR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralMutationWTPromoterSFFVAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50468PurposeGs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoterDepositorInsertrM3D-IRES-mCitrine
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Circular 200,100 (mPCSK9)
Plasmid#170122PurposeAAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promotersDepositorInsertCircular 200,100 guide RNA (Pcsk9 Mouse)
UseAAVExpressionMammalianPromoterHuman U6 and mouse U6Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-3xFLAG-gateway-EGFP
Plasmid#122845PurposeN-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTags3xFLAG and EGFPExpressionMammalianPromoterCMVAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-CANTHipi
Plasmid#53301PurposeContains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.DepositorInsertcrtO
UseLow copy number bacterial cloning vectorTags6HisPromoterTrcAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJYS3_ΔcrtYf
Plasmid#85542PurposeConstitutive transcription of FnCpf1 and crRNA of crtYfDepositorInsertsCpf1
crRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…ExpressionBacterialAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninWT
Plasmid#229709PurposeTransient expression of GFP-alpha-cateninWT in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-DDX3X
Plasmid#116730PurposeLentiviral expression of DDX3XDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-SOX2-17-p2a-KLF4
Plasmid#193290Purposeepisomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversionDepositorExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA
Plasmid#180590PurposeOpto-cytTrkBDepositorInsertTrkB
UseAAVMutationPHR(E281A)Available SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJ136
Plasmid#162665PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYAL_HLA-A2_MART1
Plasmid#171177PurposeDisplays MART-1 peptide in HLA-A*02 as a single-chain trimerDepositorInsertMART1_HLA-A2*01 single-chain trimer fusion
ExpressionBacterial and YeastPromoterGAL1Available SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
iRFP-PAmCherry1-PH_PLCd1
Plasmid#92167PurposePI(4,5)P2 single molecule fluorescent reporterDepositorInsertiRFP-PAmCherry1-PH_PLCd1
TagsiRFP-PAmCherry1ExpressionMammalianPromoterCMVAvailable SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-GFP-NMHC II-B
Plasmid#11348DepositorAvailable SinceFeb. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
phU1-CasRx-non-targeting (pKW82)
Plasmid#229050PurposeCloning backbone for CasRx crRNA (Human U1 promoter)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZP06C
Plasmid#232322PurposepZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.DepositorInsertmCherry
ExpressionBacterialPromoterrpsJAvailable SinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-pcDNA3.1 Puro-CAG-Voltron2-ST
Plasmid#172910PurposeMammalian expression of voltage sensor; soma localization targeting sequenceDepositorInsertVoltron2-ST
Tagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterCAGAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-ChR2-mCherry (AAV1)
Viral Prep#135634-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorTagsmCherryAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mtp-p53-ECFP
Plasmid#53961PurposeExpresses CFP tagged p53 in mammalian cellsDepositorAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FAH-rtTA3G
Plasmid#120309PurposeAn AAV vector that expresses rtTA3G under the c-fos promoterDepositorInsertrtTA3G
UseAAVPromoterc-fosAvailable SinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM65
Plasmid#133654PurposeIntegration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.DepositorAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
B*57:03:01
Plasmid#203260PurposeOverexpress HLA alleleDepositorInsertB*57:03:01 (HLA-B Human)
ExpressionMammalianAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti EF1a-FLAG-dCas9-VPR
Plasmid#114195PurposeLentivirus compatible dCas9-VPR construct driven by the EF1a promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianPromoterEF1aAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CRY2-mCherry
Plasmid#58368PurposeMammalian codon optimized CRY2DepositorAvailable SinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only