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Showing: 6761 - 6800 of 160936 results

  1. pAAV-Ef1a-fDIO mCherry (AAV8)

    Viral Prep
    #114471-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  2. pLenti6.2_mCherry_Grx1_roGFP2

    Plasmid
    #155045
    Purpose
    Fluorescent redox reporter
    Depositor
    Vanessa LaPointe
    Insert
    Glutaredoxin-1 (GLRX Human)
    Use
    Lentiviral
    Tags
    roGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155045-plasmid-map-sequence-id-310403

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  3. pAAV_hSynapsin_psychLight2 (AAV9)

    Viral Prep
    #163909-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV_hSynapsin_psychLight2 (#163909). In addition to the viral particles, you will also receive purified pAAV_hSynapsin_psychLight2 plasmid DNA. Synapsin-driven expression of the genetically encoded fluorescent sensor psychLight2 to detect behaviorally relevant serotonin release. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003
    Promoter
    Syn
    Tags
    None
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. mClY-N1

    Plasmid
    #90457
    Purpose
    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1
    Insert
    mClY-N1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…
    Promoter
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90457-plasmid-map-sequence-id-178580

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  12. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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  14. pHB-4

    Plasmid
    #140957
    Purpose
    For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.
    Depositor
    Kang Zhou
    Article
    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.
    Insert
    phaCAB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    140957-plasmid-map-sequence-id-334294

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  15. pICherryNeo

    Plasmid
    #52119
    Purpose
    a bicistronic IRES-mCherry containing vector for mammalian expression (CMV promoter)
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Use
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52119-plasmid-map-sequence-id-80505

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  18. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

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  22. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On 3G Bidirectional
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92099-plasmid-map-sequence-id-180428

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  33. pDAC439

    Plasmid
    #195237
    Purpose
    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195237-plasmid-map-sequence-id-382945

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  39. SpyTag-β-Lactamase-SpyCatcher

    Plasmid
    #52656
    Purpose
    Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.
    Depositor
    Mark Howarth
    Article
    Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2
    Insert
    TEM-1 β-Lactamase
    Use
    Tags
    SpyCatcher and SpyTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52656-plasmid-map-sequence-id-422741

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Showing: 6761 - 6800 of 160936 results