We narrowed to 18,717 results for: IRE

1. pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

   Plasmid

   #74513

   Purpose

   AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

   Depositor

   Guoping Feng

   Article

   Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

   Insert

   EGFP-p2A-EGFP-f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX4T-HEPN

   Plasmid

   #75296

   Purpose

   Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

   Depositor

   Kalle Gehring

   Article

   Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

   Insert

   Human Sacsin 4441-4579
   

   Use

   Tags

   GST-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FlagMBNL1-41

   Plasmid

   #96906

   Purpose

   expresses Flag tagged human MBNL1-41 kDa isoform from pcDNA3.1 HisC

   Depositor

   Thomas Cooper

   Article

   Grammatikakis et al Nucleic Acids Res. 2011 Apr;39(7):2769-8

   Insert

   MBNL1-41 kDa isoform (MBNL1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLL3.7_hsa-miR-34a

   Plasmid

   #25791

   Depositor

   Judy Lieberman

   Article

   Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

   Insert

   hsa-miR-34a (TP53 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. WT (REP10)

   Plasmid

   #21368

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pATP403red-hENT1

   Plasmid

   #166027

   Purpose

   Expresses human equilibrative nucleoside transporter 1 (hENT1)

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   human equilibrative nucleoside transporter 1 (SLC29A1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pTDH3

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. acGFP Peroxisome visual barcode

   Plasmid

   #158674

   Purpose

   tagging clonal populations with Peroxisomal acGFP fluorescent protein. Adding Peroxisome localization signal to acGFP WC

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   acGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. phMYT1L-N106

   Plasmid

   #60861

   Purpose

   Expresses MYT1L under the EF1a promoter

   Depositor

   Andrew Yoo

   Article

   Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

   Insert

   MYT1L (MYT1L Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

   Plasmid

   #62952

   Purpose

   Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   3XGal80-FLAG/HA/EGFP-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMH-Cas9-gate

    Plasmid

    #113742

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70

    Plasmid

    #62947

    Purpose

    Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-LexA::QFAD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH3449

    Plasmid

    #179576

    Purpose

    Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    BFP

    Expression

    Worm

    Mutation

    Promoter

    Plgc-55B

    Available since

    March 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pEGFP-Map7-fulllength

    Plasmid

    #46076

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-fulllength (Map7 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-yomRuby3-SpHIS5

    Plasmid

    #204287

    Purpose

    For C-terminal tagging using yeast optimized mRuby3

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pFA6-yomRuby3-SpHIS5
    

    Use

    Tags

    yomRuby3

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac-CHD7

    Plasmid

    #170141

    Purpose

    Expresses CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pF208wt-luc

    Plasmid

    #122143

    Purpose

    For luciferase expression

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Fam208a promoter (Tasor Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Fam208a

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLENTI CMV GFP TREX1 R128A R174A BLAST

    Plasmid

    #164234

    Purpose

    lentiviral plasmid for GFP-TREX1 R128A R174A

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R124A R174A

    Promoter

    CMV

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Stat1 alpha S727A pRc/CMV

    Plasmid

    #8700

    Depositor

    Jim Darnell

    Article

    Wen et al Cell 1995 Jul 28;82(2):241-50.

    Insert

    STAT1 alpha S727A (STAT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S727A

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pISH-Gap43

    Plasmid

    #74336

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

    Insert

    growth associated protein 43 (Gap43 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUWR-Cad-Venus

    Plasmid

    #58328

    Purpose

    Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-Venus
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    poly-ubiquitin

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

22. OSCA2.3

    Plasmid

    #136595

    Purpose

    Expresses the mechanosensitive channel OSCA2.3 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA2.3 (HYP1 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. acGFP NES visual barcode

    Plasmid

    #158673

    Purpose

    tagging clonal populations with NES acGFP fluorescent protein. Adding NES to acGFP WC

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    acGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVX-TetOneZeo-ORF18(WT)-3xFLAG

    Plasmid

    #129746

    Purpose

    Dox inducible expression of KSHV ORF18-3xFLAG in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3Gs and Tet inducible promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pphiLOV3-N1

    Plasmid

    #178973

    Purpose

    Expresses phiLOV3 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    phiLOV3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT-GEM(0)

    Plasmid

    #62891

    Purpose

    Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACT2-μ3A

    Plasmid

    #198174

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitution in codon 225

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pACT2-μ3B

    Plasmid

    #198175

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHR- flag-Lag16-Ecad

    Plasmid

    #205197

    Purpose

    This vector encodes of the synCAM (Ecad) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM Ecad and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Stat1 alpha Y701F Flag pRc/CMV

    Plasmid

    #8702

    Depositor

    Jim Darnell

    Article

    Wen et al Cell 1995 Jul 28;82(2):241-50.

    Insert

    STAT1 alpha Y701F (STAT1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y701F

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

31. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GFP-OSH2-2xPH

    Plasmid

    #161987

    Purpose

    Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

    Depositor

    Tamas Balla, David Bryant

    Article

    Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

    Insert

    OSH2 (OSH2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AsCpf1-2NLS

    Plasmid

    #102565

    Purpose

    bacterial expression of AsCpf1-2NLS

    Depositor

    Jennifer Doudna

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-TEV and HA-2xSV40NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EYFP Peroxisome visual barcode

    Plasmid

    #158677

    Purpose

    tagging clonal populations with Peroxisomal EYFP fluorescent protein. Adding Peroxisome localization signal to EYFP WC

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    EYFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

    Plasmid

    #62957

    Purpose

    Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4 in three phases
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70

    Plasmid

    #62896

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAOJ15

    Plasmid

    #91567

    Purpose

    Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tetracycline promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCS2 CA-LRP6-GFP

    Plasmid

    #29682

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    LDL-receptor related protein 6 (LRP6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    extracellular region deleted

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

39. Teto-FUW-Pax6

    Plasmid

    #61541

    Purpose

    Expresses Pax6 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Pax6 (Pax6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

40. XLone-SOX18

    Plasmid

    #163822

    Purpose

    expresses human SOX18 upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    SOX18 (SOX18 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET21-pelB-LaG-41-4GS-LaG-2

    Plasmid

    #172766

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70

    Plasmid

    #62912

    Purpose

    Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-p65AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. FUW-TetO-Gata4

    Plasmid

    #41084

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

    Insert

    Gata4 (Gata4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO + minimal CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET21-pelB-LaM-2

    Plasmid

    #172768

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pKSJ376

    Plasmid

    #103065

    Purpose

    Expresses colicin E1 with R domain deletion and ImmE1

    Depositor

    Alan Finkelstein, Karen Jakes

    Article

    Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

    Inserts

    colicin E1 immunity protein (pColE1_26, imm E. coli)
    cea/colicin E1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R (receptor-binding domain) deletion in colicin E…

    Promoter

    T7 lac (from pET) and T7 lac (from pET52-b)

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pMXs_FLAG-NMNAT2

    Plasmid

    #133260

    Purpose

    Express FLAG-tagged NMNAT2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NMNAT2 (NMNAT2 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDUP1

    Plasmid

    #223516

    Purpose

    inactive cogfp under control of Ptet, cogfp under control of Ptac

    Depositor

    Yolanda Schaerli

    Article

    Ljiljana et al eLife13:RP97216

    Inserts

    CoGFP
    CoGFP inactivated
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    anti-dimerization mutations S129G, 22 C154S, D156…

    Promoter

    Ptac, Ptac and Ptet, and Ptet

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pIVT/Smarca4

    Plasmid

    #122146

    Purpose

    For making Smarca4 RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Smarca4 (Smarca4 Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. AMPK-β1-FLAG

    Plasmid

    #40602

    Depositor

    Harvey Lodish

    Article

    Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

    Insert

    AMPK-β1 (Prkab1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

50. pET21-pelB-LaG-10

    Plasmid

    #172743

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-10
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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