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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,362 results for: Coli

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Showing: 5101 - 5130 of 10362 results

  1. pSCL.180

    Plasmid
    #185001
    Purpose
    Express -Eco1 AAVS1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    AAVS1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185001-plasmid-map-sequence-id-376682

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  3. pRCPam

    Plasmid
    #195861
    Purpose
    Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Insert
    Amber Chromogenic Protein on pRCPam plasmid
    Expression
    Bacterial
    Mutation
    Gln62X
    Available Since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195861-plasmid-map-sequence-id-384172

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  4. pAd5-Blue

    Plasmid
    #174431
    Purpose
    pAd5-Blue provides a platform for the rapid construction of recombinant and replication defective Adenovirus. This vector contains unique restriction enzyme sites to allow cloning of a gene.
    Depositor
    Teresa De Los Santos
    Article
    Fernandez-Sainz et al Virology. 2017 Feb;502:123-132. doi: 10.
    Insert
    β-galactosidase α gene fragment
    Use
    Adenoviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    174431-plasmid-map-sequence-id-365370

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  16. pPPC001

    Plasmid
    #171138
    Purpose
    For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171138-plasmid-map-sequence-id-344761

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  19. pZJQIBEBT003

    Plasmid
    #140053
    Purpose
    The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.
    Depositor
    Jia Zhang
    Article
    Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952
    Insert
    eMutS-L157C-G233C-CBM-EGFP
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    L157C, G233C
    Promoter
    T7
    Available Since
    June 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    140053-plasmid-map-sequence-id-296195

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  23. pT7-agrBD-IV

    Plasmid
    #53440
    Purpose
    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
    Depositor
    Cynthia Collins
    Article
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus (with D29Y mutation in agrD)
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    D to Y mutation of residue 29 of the agrD gene (c…
    Promoter
    T7-lac
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53440-plasmid-map-sequence-id-83235

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  25. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137039-plasmid-map-sequence-id-291948

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  27. p1585588

    Plasmid
    #62620
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    dihydroflavonol 4-reductase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62620-plasmid-map-sequence-id-103534

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  29. pMM549

    Plasmid
    #112531
    Purpose
    pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR
    Depositor
    Timothy Lu
    Article
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    Expression
    Bacterial
    Promoter
    J23107 and ProD
    Available Since
    Feb. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    112531-plasmid-map-sequence-id-217579

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Showing: 5101 - 5130 of 10362 results