We narrowed to 11,252 results for: NSI
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mEos3.2trLAT-2P
Plasmid#203748PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.DepositorInserttrLAToC27AC30A (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationC27A, C30APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP4-1/1
Plasmid#91518PurposeProtein expression and purification of human SH3 domain construct ARHGAP4-1/1DepositorInsertARHGAP4-1/1 (ARHGAP4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP9-1/1
Plasmid#91442PurposeProtein expression and purification of human SH3 domain construct ARHGAP9-1/1DepositorInsertARHGAP9-1/1 (ARHGAP9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains1
Plasmid#78461PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2149-2170; Q2513R (please see…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Tet1 promoter B
Plasmid#63882PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet1DepositorInsertTet1 promoter fragment B
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA
Plasmid#140014PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
UseTagsFLAGExpressionMammalianMutationY126A, Y197FPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo_eGFP_V5
Plasmid#160096PurposeExpresses eGFP in mammalian cellsDepositorInserteGFP
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-SPASTIN, F124D
Plasmid#180648PurposeLentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, F124D mutation, has siRNA resistan…PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVL47 - pSCR>>
Plasmid#116013Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p260 pCMV-CRE-M-AR(wt)
Plasmid#12494DepositorInsertCRE-M AR (AR Human)
UseCre/LoxTagsExpressionMammalianMutationCRE-M and AR ligand binding domainPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G_NT-GSDMD_I105N_
Plasmid#201174PurposeDox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D N-terminal domain (Gsdmd Mouse)
UseRetroviralTagsExpressionMutationaa1-276 only, I105NPromoterTRE3GAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
UseTags6His-TEVExpressionBacterialMutationR49APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)
Plasmid#39858DepositorInsertC11orf51 (ANAPC15 Human)
UseTagsIRES2-mRubyExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
TRS6E1b-luc(-732/-721) mutant 5
Plasmid#45388DepositorInsert6 copies of hPAI-1 promoter -732/-721 mutated at -725/723
UseLuciferaseTagsluciferaseExpressionMammalianMutation-725/-723 mutated from gtt to cccPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
Emerald-MannII-N-10
Plasmid#54150DepositorInsertMannII (Man2a1 Mouse)
UseTagsEmeraldExpressionMammalianMutationaa 1-112 of MannII aa 1-112 of MannIIPromoterCMVAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBW_0001
Plasmid#102833PurposeBinary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminatorDepositorInsertSr22 Schomburgk (NEWENTRY Synthetic)
UseTagsExpressionPlantMutationPromoterMaize ubiquitinAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pNCST-AausFP3
Plasmid#129501PurposeExpresses AausFP3 constitutively in E. coli (most strains)DepositorInsertAausFP3
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FUT8-1/1
Plasmid#91270PurposeProtein expression and purification of human SH3 domain construct FUT8-1/1DepositorInsertFUT8-1/1 (FUT8 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE Tight ArchT-GFP
Plasmid#79627PurposeTetracycline-dependent expression of ArchT-EGFP in mammalian cellsDepositorInsertArchT
UseTagsEGFPExpressionMammalianMutationArchT is a more sensitive version of Arch (archae…PromoterTet responsive Ptight promoterAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVL46 - pWOX4>>
Plasmid#116012Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-KD
Plasmid#140312PurposeExpresses HA-tagged human Src-KD in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationContains only the KD regionPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-D2512H
Plasmid#69017Purposeactivating MTOR mutationDepositorInsertMTOR-D2512H (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Aspartate 2512 to HistidinePromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GSDMD_NoCys-Myc
Plasmid#201169PurposeExpression of GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D (Gsdmd Mouse)
UseRetroviralTagsMycExpressionMutationC39A, C57A, C77A, C122A, C265A, C192APromoterMESVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_HSP7C
Plasmid#111667PurposeMAC-tagged gene expressionDepositorInsertHSP7C (HSPA8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR
Plasmid#75136PurposeRetroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9)DepositorInsertshBrd9_1116
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR
Plasmid#75135PurposeRetroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9)DepositorInsertshBrd9_1061
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL-p21UTRm1
Plasmid#20878DepositorInsertp21 3'UTR site 1 mutation (Cdkn1a Mouse)
UseLuciferaseTagsExpressionMammalianMutationPredicted miRNA binding site 1 (Position ~420) GC…PromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GAS7-1/1
Plasmid#91301PurposeProtein expression and purification of human SH3 domain construct GAS7-1/1DepositorInsertGAS7-1/1 (GAS7 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP4
Plasmid#129502PurposeExpresses AausFP4 constitutively in E. coli (most strains)DepositorInsertAausFP4
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S100C
Plasmid#190010PurposeExpression of human TTR in E coliDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S100CPromotert7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_CDK7
Plasmid#111684PurposeMAC-tagged gene expressionDepositorInsertCDK7 (CDK7 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-UD
Plasmid#140304PurposeExpresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationcontains only the SH4 and UD regionsPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-KD
Plasmid#140296PurposeExpresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationcontains only the SH4 and KD regionsPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-UD-KD
Plasmid#140300PurposeExpresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationlacks the SH3 and SH2 domainsPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQC hKGA.wt V5-His IRES G418
Plasmid#110390Purposeγ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.DepositorInsertKGA glutaminase (GLS Human)
UseRetroviralTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMADM-alpha
Plasmid#36890DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, deleted…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN Incenp
Plasmid#108481Purposeinducible expression of vsv-CenpB-1-166-dCEN-INCENPDepositorInsertCenpB-dCEN-INCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationCEN box replaced by CenpB 1-166PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only
