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291,538 results

Showing: 8701 - 8750 of 291538 results

  9. pAAV-hSyn-EGFP (AAV PHP.eB)

    Viral prep
    #50465-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only

  10. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190033
    Purpose
    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized hfCas13X
    Expression
    Mammalian
    Mutation
    Y672A,Y676A
    Promoter
    CBh, SV40, hU6
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    190033-plasmid-map-sequence-id-488812

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  11. pLenti-mCherry-Cre-blast

    Plasmid
    #179390
    Purpose
    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.
    Depositor
    Moshe Szyf
    Publication
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    mCherry (P2A) Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    179390-plasmid-map-sequence-id-451660

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  12. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)

    Viral prep
    #162378-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only

  13. pGP-AAV-syn-jGCaMP8s-WPRE (AAV Retrograde)

    Viral prep
    #162374-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)

    Viral prep
    #154867-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Publication
    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Publication
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  18. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Publication
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  23. pMal-T-Avi-His/BirA

    Plasmid
    #102962
    Purpose
    Vector for in vivo biotinylation of a fusion protein in bacteria.
    Depositor
    Tonia Rex
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    102962-plasmid-map-sequence-id-199098

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  24. pMal-T-Avi-His/BirA

    Plasmid
    #102962
    Purpose
    Vector for in vivo biotinylation of a fusion protein in bacteria.
    Depositor
    Tonia Rex
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    102962-plasmid-map-sequence-id-199098

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  27. pSNAPf-H2B Control Plasmid

    Plasmid
    #101124
    Purpose
    Expression of SNAP-tagged Histone H2B in mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    histone cluster 1 H2B family member j (H2BC11 Human)
    Tags
    SNAP-tag (SNAPf)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    4 Articles
    101124-plasmid-map-sequence-id-194083

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  28. pSNAPf-H2B Control Plasmid

    Plasmid
    #101124
    Purpose
    Expression of SNAP-tagged Histone H2B in mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    histone cluster 1 H2B family member j (H2BC11 Human)
    Tags
    SNAP-tag (SNAPf)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    4 Articles
    101124-plasmid-map-sequence-id-194083

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  29. pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

    Plasmid
    #92142
    Purpose
    Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    osTir1
    Use
    Mouse Targeting
    Tags
    V5 tag
    Expression
    Mammalian
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    92142-plasmid-map-sequence-id-194468

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  30. pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

    Plasmid
    #92142
    Purpose
    Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    osTir1
    Use
    Mouse Targeting
    Tags
    V5 tag
    Expression
    Mammalian
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    92142-plasmid-map-sequence-id-194468

    Enlarge

  31. pcDNA3-PRB

    Plasmid
    #89130
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418
    Depositor
    Elizabeth Wilson
    Publication
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human progesterone receptor-B (PGR Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    89130-plasmid-map-sequence-id-176077

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  32. pcDNA3-PRB

    Plasmid
    #89130
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418
    Depositor
    Elizabeth Wilson
    Publication
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human progesterone receptor-B (PGR Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    89130-plasmid-map-sequence-id-176077

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  42. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Bob Goldstein
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    70684-plasmid-map-sequence-id-306412

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  43. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Bob Goldstein
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    70684-plasmid-map-sequence-id-306412

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  44. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65773-plasmid-map-sequence-id-112657

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  45. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65773-plasmid-map-sequence-id-112657

    Enlarge

  48. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNase HI-D10R-E48R
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    60367-plasmid-map-sequence-id-456413

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  49. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNase HI-D10R-E48R
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    60367-plasmid-map-sequence-id-456413

    Enlarge

Showing: 8701 - 8750 of 291538 results