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301,789 results

Showing: 8701 - 8750 of 301789 results

  5. pD649-HAsp-EPCAM-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156693
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    EPCAM (EPCAM Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A mā€¦
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156693-plasmid-map-sequence-id-417861

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  7. pcDNA3-neo-cterminal-3HA

    Plasmid
    #102643
    Purpose
    To generate three copies of the HA epitope tag on cDNAs.
    Depositor
    Michael E. Wright
    Type
    Empty backbone
    Tags
    3x HA (YPYDVPDYA)
    Expression
    Mammalian
    Available Since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    102643-plasmid-map-sequence-id-196911

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  8. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Article
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has Service
    Cloning Grade DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71814-plasmid-map-sequence-id-177023

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  14. TFORF3545

    Plasmid
    #145021
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PIAS1 (PIAS1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145021-plasmid-map-sequence-id-287177

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  21. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid
    #212020
    Purpose
    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ā€˜=ā€™).
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    UtrCH
    Tags
    mStayGold(c4)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212020-plasmid-map-sequence-id-419202

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  22. pLenti.CAG.H2B-Dendra2.W

    Plasmid
    #51005
    Purpose
    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-Dendra2
    Use
    Lentiviral
    Tags
    histone2B
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51005-plasmid-map-sequence-id-490664

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  24. mEmerald-H2B-N-22

    Plasmid
    #54114
    Purpose
    Localization: Nucleus/Histones, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

    Plasmid
    #246438
    Purpose
    All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    NmeCas9
    Nme-sgRNA
    Tags
    HA tag, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    changed Aspartic acid 16 to Alanine, deleted aminā€¦
    Promoter
    EF-1 alpha and U6
    Available Since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246438-plasmid-map-sequence-id-490930

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  31. pSBbi-pur H-2Kb

    Plasmid
    #111623
    Purpose
    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele
    Depositor
    Jon Yewdell
    Insert
    H2Kb
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111623-plasmid-map-sequence-id-213220

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  32. GLP1R-DuET

    Plasmid
    #213253
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GLP1R (GLP1R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213253-plasmid-map-sequence-id-430803

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  33. pRH2502

    Plasmid
    #84379
    Purpose
    Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter
    Depositor
    Robert Husson
    Article
    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw
    Insert
    dcas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Aspartate at position 10 changed to Alanine, Histā€¦
    Promoter
    uv15tetO
    Available Since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    84379-plasmid-map-sequence-id-173256

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  34. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

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  36. pTripZ EV

    Plasmid
    #209071
    Purpose
    DOX-ON inducible expression vector that has a stuffer sequence after an LR reaction and used as empty expression vector control
    Depositor
    Matthew Oser
    Article
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Insert
    stuffer DNA sequence after LR recombination with pTripZ NEO DEST to be used as an empty vector control
    Use
    Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    209071-plasmid-map-sequence-id-412293

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  43. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    Eric Lander, David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

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  45. pTRKH2

    Plasmid
    #71312
    Purpose
    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.
    Depositor
    Rodolphe Barrangou, Todd Klaenhammer
    Article
    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.
    Type
    Empty backbone
    Use
    E. coli-gram positive bacteria shuttle vector
    Expression
    Bacterial
    Available Since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    71312-plasmid-map-sequence-id-323567

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Showing: 8701 - 8750 of 301789 results