We narrowed to 17,911 results for: REV

1. pHH0103_SH3RF1-4/4

   Plasmid

   #91374

   Purpose

   Protein expression and purification of human SH3 domain construct SH3RF1-4/4

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3RF1-4/4 (SH3RF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHH0103_SH3RF3-2/2

   Plasmid

   #91356

   Purpose

   Protein expression and purification of human SH3 domain construct SH3RF3-2/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3RF3-2/2 (SH3RF3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHH0103_SH3RF1-3/4

   Plasmid

   #91366

   Purpose

   Protein expression and purification of human SH3 domain construct SH3RF1-3/4

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3RF1-3/4 (SH3RF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHH0103_SH3RF3-1/2

   Plasmid

   #91280

   Purpose

   Protein expression and purification of human SH3 domain construct SH3RF3-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3RF3-1/2 (SH3RF3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. px330-MRPsg4

   Plasmid

   #87192

   Purpose

   disruption of human RMRP gene via CRISPR-Cas9, guide 4

   Depositor

   Thomas Cech

   Article

   Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

   Insert

   human MRP RNA CRISPR guide #4 (RMRP Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDF1-ERBB4 (E452K)

   Plasmid

   #29538

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pKP4010

   Plasmid

   #113336

   Purpose

   Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

   Inserts

   Artificial Transcription Factor (McIsaac et al, 2013)
   Ty3 Integrase
   

   Use

   Tags

   3X-FLAG/8X-Gly linker

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKV005

   Plasmid

   #64763

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable Myc tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.2/V5 mmu-mir-139

   Plasmid

   #26329

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   mmu-mir-139 (Mir139 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. roGFP_iE_C204S_pQE30

    Plasmid

    #48632

    Purpose

    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Serine mutation (roGFP C147S, E147b, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    roGFP1
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    insertion E147B, c204S

    Promoter

    Available since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCerulean3-H2B-C-10

    Plasmid

    #55422

    Purpose

    Localization: Nucleus/Histones, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pY71-tet.I.16

    Plasmid

    #133536

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

    Plasmid

    #58830

    Purpose

    Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

    Insert

    2 x Osh2 PH domain (OSH2 Budding Yeast)
    

    Use

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    changed Lysine 307 to Glutamate and Arginine 309 …

    Promoter

    PHO5

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pBS-miR106a-363

    Plasmid

    #19028

    Depositor

    Tyler Jacks

    Article

    Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

    Insert

    microRNA 106a-363 (Mir106a Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_SH3BP4-1/1

    Plasmid

    #91532

    Purpose

    Protein expression and purification of human SH3 domain construct SH3BP4-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3BP4-1/1 (SH3BP4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103_SORBS2-1/3

    Plasmid

    #91387

    Purpose

    Protein expression and purification of human SH3 domain construct SORBS2-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SORBS2-1/3 (SORBS2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_VAV1-1/2

    Plasmid

    #91355

    Purpose

    Protein expression and purification of human SH3 domain construct VAV1-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV1-1/2 (VAV1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.2/V5 mmu-mir-1224

    Plasmid

    #26337

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-1224 (Mir1224 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pRS313-PRP8

    Plasmid

    #90964

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    native

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 720-2µ-maxHIS3

    Plasmid

    #40612

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Budding Yeast, Synthetic)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Every codon of the coding sequence has been repla…

    Promoter

    TDH3

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

    Plasmid

    #116885

    Purpose

    FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

    Depositor

    Brian Kuhlman

    Article

    Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

    Insert

    LINXa4(RVH)-Bre1
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

    Promoter

    ADH1

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_STAM-1/1

    Plasmid

    #91428

    Purpose

    Protein expression and purification of human SH3 domain construct STAM-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    STAM-1/1 (STAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC 3GLA UAS HAi

    Plasmid

    #71763

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX-4T-1 dSesn (1187)

    Plasmid

    #66919

    Purpose

    bacterial expression of dSesn

    Depositor

    Michael Karin, Jun Hee Lee

    Article

    Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

    Insert

    Sesn (Sesn Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. sh-Myocardin

    Plasmid

    #100768

    Purpose

    expression of shRNA targeting MYOCD

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    sh-Myocardin
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHH0103_PACSIN3-1/1

    Plasmid

    #91335

    Purpose

    Protein expression and purification of human SH3 domain construct PACSIN3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PACSIN3-1/1 (PACSIN3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-RsACR_995-EYFP

    Plasmid

    #103772

    Purpose

    Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin RsACR_995
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-GcACR_457-EYFP

    Plasmid

    #103137

    Purpose

    Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin GcACR_457
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOT2 3GLA

    Plasmid

    #71764

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MDH1-PGK-FLAG-TAT-SIVmac251

    Plasmid

    #35979

    Depositor

    Susana Valente

    Article

    Mediouni et al Curr HIV Res. 2015;13(1):64-79.

    Insert

    transactivating protein
    

    Use

    RNAi and Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    SIV strain mac251

    Promoter

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCfB2801

    Plasmid

    #63656

    Purpose

    pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDF1-ERBB4 (E563K)

    Plasmid

    #29541

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pRS426-TUB1-internal6xHis (1-440)

    Plasmid

    #60391

    Purpose

    Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4), amino a…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

34. MPDZ_PDZ_7

    Plasmid

    #103891

    Purpose

    Protein expression and purification of Multiple PDZ protein PDZ7 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MPDZ (MPDZ Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MPDZ_PDZ_13

    Plasmid

    #103897

    Purpose

    Protein expression and purification of Multiple PDZ protein PDZ13 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MPDZ (MPDZ Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.2/V5 mmu-mir-217

    Plasmid

    #26333

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-217 (Mir217 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

37. MPDZ_PDZ_3

    Plasmid

    #103887

    Purpose

    Protein expression and purification of Multiple PDZ protein PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MPDZ (MPDZ Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFP122

    Plasmid

    #21669

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pDONR221-rap1-K651R

    Plasmid

    #84528

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    K651R

    Promoter

    none

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pYG026

    Plasmid

    #65328

    Purpose

    reporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    ccdB
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRY1004

    Plasmid

    #21788

    Depositor

    Richard Yu

    Article

    Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

    Insert

    Dig1-YFP (DIG1 Budding Yeast, A. victoria)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    contains 5' 1000bp UTR (Dig1 promoter) Di…

    Promoter

    Available since

    Sept. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1-R1ACR_741-EYFP

    Plasmid

    #103961

    Purpose

    Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R1ACR_741
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pDONR-IAA17_no stop

    Plasmid

    #83855

    Purpose

    Gateway donor vector for IAA17 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. mCerulean3-Dectin1A-N-10

    Plasmid

    #55414

    Purpose

    Localization: Membrane, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Dectin1A (CLEC7A Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

    Plasmid

    #68280

    Purpose

    SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Mkk2DS NLS mCherry (MKK2 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    RPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCfB2991

    Plasmid

    #63643

    Purpose

    pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. pKV006

    Plasmid

    #64764

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable V5 tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. A337C/Y344C

    Plasmid

    #24434

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

49. pST39-His/Vps27(345-440) - Hse(275-375)

    Plasmid

    #17638

    Depositor

    James Hurley

    Article

    Prag et al Dev Cell. 2007 Jun . 12(6):973-86.

    Insert

    His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

50. K222C/E334C

    Plasmid

    #24435

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E…

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only