We narrowed to 16,562 results for: IGH@

1. CLIPf-KIF5A-SNAPf-HisTag-FLAG

   Plasmid

   #200789

   Purpose

   Mammalian expression of CLIPf-KIF5A-SNAPf-HisTag-FLAG

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   CLIPf and SNAPf, HisTag, FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV & T7

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. KIF5A-SNAPf-HisTag-FLAG

   Plasmid

   #200791

   Purpose

   Mammalian expression of KIF5A-SNAPf-HisTag-FLAG

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   SNAPf, HisTag, FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV & T7

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CLIPf-KIF5B-SNAPf-HisTag-FLAG

   Plasmid

   #200799

   Purpose

   Mammalian expression of CLIPf-KIF5B-SNAPf-HisTag-FLAG

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   KIF5B (KIF5B Human)
   

   Use

   Tags

   CLIPf and SNAPf, HisTag, FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV & T7

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSMART-HCKan-yibDp-VHH-GFPenhancer

   Plasmid

   #202468

   Purpose

   Expresses the nanobody GFP enhancer after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody GFP enhancer
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSMART-HCKan-yibDp-VHH-GFPminimizer

   Plasmid

   #202469

   Purpose

   Expresses the nanobody GFP minimizer after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody GFP minimizer
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSMART-HCKan-yibDp-roGFP2

   Plasmid

   #202462

   Purpose

   Expresses redox sensitive GFP (roGFP) after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   reduction-oxidation sensitive green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDB179-LCC-ICCG-I6M

   Plasmid

   #218690

   Purpose

   Encodes codon optimized LCC-ICCG I6M enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   LCC-ICCG
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. WDV

   Plasmid

   #226983

   Purpose

   Expresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experiments

   Depositor

   Wendy Gordon

   Article

   Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

   Insert

   WDV (repA )
   

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBWB514

   Plasmid

   #209326

   Purpose

   p15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.

   Depositor

   Adam Arkin

   Article

   Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+) Rheb(WT)

    Plasmid

    #224289

    Purpose

    Cell transfection and expression of Rheb

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rheb (RHEB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDB179-PES-H1-L92F-Q94Y

    Plasmid

    #218705

    Purpose

    Encodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    PES-H1
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    L92F-Q94Y

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p2H12ref

    Plasmid

    #221188

    Purpose

    cdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    None (same as cdGreen) and Ptet

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRER-PhoCl-Insert

    Plasmid

    #219675

    Purpose

    Caging at lumenal N-ter of POI: TMEM-PhoCl-MCS in pTREtight2

    Depositor

    Helge Ewers

    Article

    Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

    Insert

    RER-PhoCl
    

    Use

    Tags

    PhoCl

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCDB179-LCC-ICCG-DAQI

    Plasmid

    #218689

    Purpose

    Encodes codon optimized LCC-ICCG DAQI enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG-DAQI
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDB179-TfCut2-L32E-S113E-T237Q

    Plasmid

    #218696

    Purpose

    Encodes codon optimized TfCut2 L32E-S113E-T237Q enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TfCut2
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    L32E-S113E-T237Q

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDB179-LCC-ICCG-RIP

    Plasmid

    #218688

    Purpose

    Encodes codon optimized LCC-ICCG RIP enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG-RIP
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pITEdv1

    Plasmid

    #218283

    Purpose

    Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pITEu2

    Plasmid

    #218282

    Purpose

    Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJE13HD7

    Plasmid

    #218281

    Purpose

    Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA scaffold-spacer-mouse U6

    Plasmid

    #214698

    Purpose

    provide "sgRNA scaffold-spacer-mouse U6" fragment

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    sgRNA scaffold-spacer-mouse U6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-REPORT (PGK) 12xHRE-NanoLuc

    Plasmid

    #208575

    Purpose

    hypoxic response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    NanoLuciferase
    d2eGFP
    12xHRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

    Plasmid

    #207171

    Purpose

    expression plasmid for Gal4DBD-HIF-CAD.myc expression

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    Gal4DBD-HIF1a-CAD Myc tag linker
    

    Use

    Tags

    2xMyc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker

    Plasmid

    #207172

    Purpose

    expression plasmid for Gal4DBD-HIF-CAD expression GalO linker

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    Gal4DBD-HIF1a-CAD GalO linker
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28_human-pro-IL-18_E192K_D193K

    Plasmid

    #214322

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    E192K_D193K

    Promoter

    T7

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28_human-pro-IL-18_I48N

    Plasmid

    #214323

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    I48N

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNS49 (enAsCas12a-KRAB piggyBac)

    Plasmid

    #217335

    Purpose

    piggyBac expresion of enAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    enAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYPQ262F-ABE8e

    Plasmid

    #199179

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Physiol. 2023 Aug 31;193(1):291-30

    Insert

    ABE8e(V106W)-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a_mouse-Pro-IL-18_humanized-triple-mutant

    Plasmid

    #214339

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (Il18 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    ENGD29DDEN_N36Y_H41E

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28_human-pro-IL-18_LES33AAA

    Plasmid

    #214326

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    LES33AAA

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28_human-pro-IL-18_DDEN29ENGD

    Plasmid

    #214325

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    DDEN29ENGD

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28_human-pro-IL-18_V47N_I48N

    Plasmid

    #214324

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    V47N_I48N

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a_Pro-IL-18_rat

    Plasmid

    #214333

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (Il18 Rat)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 3H-α-synuclein

    Plasmid

    #205714

    Purpose

    Mammalian expression of 3H-alpha-synuclein

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    thermostable three helix-bundle protein 3H

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. α-synuclein-3H

    Plasmid

    #205715

    Purpose

    Mammalian expression of alpha-synuclein-3H

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    thermostable three helix-bundle protein 3H

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-αS-2

    Plasmid

    #205706

    Purpose

    Mammalian expression of alpha-synuclein tandem dimer

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein tandem dimer (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-hsyn-dMASS_1_WPRE

    Plasmid

    #209765

    Purpose

    AAV virus production for neuronal expression of dMASS_1 under hSyn promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV.CAGFLEX.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209656

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95K

    Promoter

    CAG

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNBA001

    Plasmid

    #155323

    Purpose

    Binary vector for expression of PULSE-controlled EFR-GFP

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_(etr)8-(C120)5-PhCMVmin-EFR-GFP-T35S_RB
    

    Use

    Synthetic Biology; Eukaryotic expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNBA002

    Plasmid

    #155324

    Purpose

    Binary vector for expression of PULSE-controlled GBP-mCherry

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_(etr)8-(C120)5-PhCMVmin-GBP-mCherry-T35S_RB
    

    Use

    Synthetic Biology; Eukaryotic expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pROF018

    Plasmid

    #155325

    Purpose

    Constitutive expression of KRAB-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-NLS-KRAB-EL222-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pROF021

    Plasmid

    #155326

    Purpose

    Expression of PULSE-controlled FLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-FLuc-TSV40
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pROF050

    Plasmid

    #155327

    Purpose

    Constitutive expression of BRD-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-BRD-NLS-EL222-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSC-cUnaG-LPETG

    Plasmid

    #207635

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

    Plasmid

    #206213

    Purpose

    PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

    Depositor

    Masamichi Kamihira

    Article

    Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

    Insert

    tetracycline-responsive transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAV-CAG-EGFPnls barcode-210-SV40 polyA

    Plasmid

    #190876

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFPnls-barcode210
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAAV-CAG-EGFP barcode-9-SV40 polyA

    Plasmid

    #190873

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. eSrtA-MT4

    Plasmid

    #200306

    Purpose

    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    eSrtA-LPETG-MT4 (MT4 Human)
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLV-REPORT(PGK/CMV)-GRE

    Plasmid

    #172336

    Purpose

    Gal4 Response element

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. SUMO_LPETG_MT3

    Plasmid

    #200304

    Purpose

    Bacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavage

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    metallothionein 3 (MT3 Human)
    

    Use

    Tags

    Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. SUMO_MT3

    Plasmid

    #200303

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    metallothionein 3 (MT3 Human)
    

    Use

    Tags

    Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge