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171,853 results

Showing: 9901 - 9950 of 171853 results

  1. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)

    Viral Prep
    #112677-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available Since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  7. pCK01_PalkS_alkS

    Plasmid
    #166502
    Purpose
    Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.
    Depositor
    Sven Panke
    Article
    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2
    Insert
    alkS
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    L401G
    Promoter
    PalkS
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    166502-plasmid-map-sequence-id-333027

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  15. p-mCherry-C1-iC++_CA

    Plasmid
    #98168
    Purpose
    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iC++_C128A gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, V24…
    Promoter
    CMV (+enhancer)
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98168-plasmid-map-sequence-id-182759

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  21. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  22. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100846-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (#100846). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    CAG
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  23. pXR003_puroR

    Plasmid
    #219819
    Purpose
    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219819-plasmid-map-sequence-id-440390

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  29. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

    Plasmid
    #171383
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Article
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Insert
    humanized minidCas13X.1-REPAIRv2
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171383-plasmid-map-sequence-id-337350

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  38. Joint Modular Cloning (JMC) Toolkit

    Plasmid Kit
    #1000000233
    Purpose
    Modular cloning chassis and set of functional parts for assembling plant transformation vectors, compatible with MoClo.
    Depositor
    Daniel Voytas
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate
    Available Since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only

  40. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP (AAV Retrograde)

    Viral Prep
    #55639-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO hChR2(H134R)-EYFP (#55639). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO hChR2(H134R)-EYFP plasmid DNA. Ef1a-driven, Flp-dependent expression of ChR2(H134R)-EYFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only

  46. p667-UBC-BCKDK-V5-miniTurbo_IDG-K

    Plasmid
    #158189
    Purpose
    Gateway destination clone of BCKDK (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    BCKDK-V5-miniTurbo (BCKDK Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    158189-plasmid-map-sequence-id-328918

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  50. pUbiGUSPlus

    Plasmid
    #64402
    Purpose
    Ubiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.
    Depositor
    Claudia Vickers
    Article
    Vickers et al Plant Cell Rep. 2003 Sep;22(2):135-40. E
    Insert
    ubiquitin (Ubi-1) promoter
    Tags
    GUSPlus reporter
    Expression
    Plant
    Promoter
    Ubi-1
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64402-plasmid-map-sequence-id-108183

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Showing: 9901 - 9950 of 171853 results