Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

165,637 results

Showing: 51 - 100 of 165637 results

  1. LSB-hsa-let-7f-1-3p

    Plasmid
    #103154
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7f-1-3p target (MIRLET7F1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103154-plasmid-map-sequence-id-213240

    Enlarge

  4. pCMV-VSV-G

    Plasmid
    #8454
    Purpose
    Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    8454-plasmid-map-sequence-id-404044

    Enlarge

  7. pAAV2/8

    Plasmid
    #112864
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap8
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Truncated P5
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112864-plasmid-map-sequence-id-333531

    Enlarge

  9. pGP-AAV-syn-jGCaMP8m-WPRE (AAV9)

    Viral Prep
    #162375-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV1)

    Viral Prep
    #105553-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    None
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  11. pRL-SV40P

    Plasmid
    #27163
    Purpose
    Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments
    Depositor
    Ron Prywes
    Article
    Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702
    Insert
    SV40 promoter
    Use
    Luciferase
    Tags
    Renilla Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2011
    Availability
    Academic Institutions and Nonprofits only
    27163-plasmid-map-sequence-id-419582

    Enlarge

  14. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV5)

    Viral Prep
    #20298-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV.TBG.PI.eGFP.WPRE.bGH (AAV8)

    Viral Prep
    #105535-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.TBG.PI.eGFP.WPRE.bGH (#105535). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.eGFP.WPRE.bGH plasmid DNA. TBG-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    TBG
    Tags
    EGFP
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV-hSyn-EGFP (AAV9)

    Viral Prep
    #50465-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only

  19. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

    Enlarge

  21. pAAV-hSyn-DIO-mCherry (AAV2)

    Viral Prep
    #50459-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  23. pAAV-hSyn-mCherry (AAV Retrograde)

    Viral Prep
    #114472-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-FLEX-tdTomato (AAV9)

    Viral Prep
    #28306-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (Cre-dependent)
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  30. pAAV2/9n

    Plasmid
    #112865
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap9
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Truncated P5
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112865-plasmid-map-sequence-id-350300

    Enlarge

  32. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV9)

    Viral Prep
    #105553-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    None
    Available since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only

  34. pAAV-hSyn-GRAB_ACh3.0 (AAV9)

    Viral Prep
    #121922-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_ACh3.0 (#121922). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_ACh3.0 plasmid DNA. Syn-driven expression of the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s
    Promoter
    Syn
    Tags
    None
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  35. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV2)

    Viral Prep
    #44361-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  38. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    99154-plasmid-map-sequence-id-352449

    Enlarge

  40. pAAV-GFAP-hM3D(Gq)-mCherry (AAV5)

    Viral Prep
    #50478-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-GFAP-hM3D(Gq)-mCherry (#50478). In addition to the viral particles, you will also receive purified pAAV-GFAP-hM3D(Gq)-mCherry plasmid DNA. GFAP-driven hM4D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    GFAP
    Tags
    mCherry
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only

  44. tFucci(CA)5

    Plasmid
    #153521
    Purpose
    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.
    Insert
    tFucci(CA)5.
    Use
    Tags
    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    153521-plasmid-map-sequence-id-308996

    Enlarge

  45. eeBxb1

    Plasmid
    #222339
    Purpose
    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells
    Depositor
    David Liu
    Article
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    222339-plasmid-map-sequence-id-473210

    Enlarge

  46. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV Retrograde)

    Viral Prep
    #105553-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    None
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  48. pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (AAV9)

    Viral Prep
    #100851-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (#100851). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Tags
    None
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  50. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52962-plasmid-map-sequence-id-322376

    Enlarge

Showing: 51 - 100 of 165637 results