167,966 results

1. Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)

   Pooled Library

   #1000000048

   Purpose

   CRISPR gRNA knockout pooled library for targeting the human genome

   Depositor

   Feng Zhang

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

2. HA-HIF2alpha-pcDNA3

   Plasmid

   #18950

   Depositor

   William Kaelin

   Article

   Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL2-p21 promoter-Luc

   Plasmid

   #33021

   Depositor

   Martin Walsh

   Article

   Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

   Insert

   p21 promoter (CDKN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   p21

   Available Since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-hALIX

   Plasmid

   #21504

   Depositor

   James Hurley

   Article

   Lee et al Science. 2008 Oct 24. 322(5901):576-80.

   Insert

   ALIX (PDCD6IP Human)
   

   Tags

   Cherry

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. Jacquere (split-vector)

   Pooled Library

   #247026

   Purpose

   Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.

   Depositor

   David Root, John Doench

   Article

   Drepanos et al bioRxiv 2025.08.26.672375; doi: https://

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

6. Tet1-dCas9

   Plasmid

   #136650

   Purpose

   Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Tet1-dCas9-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Jacquere (all-in-one vector)

   Pooled Library

   #247027

   Purpose

   Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.

   Depositor

   David Root, John Doench

   Article

   Drepanos et al bioRxiv 2025.08.26.672375; doi: https://

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

8. AAVS1 T2 CRIPR in pX330

   Plasmid

   #72833

   Purpose

   CRISPR/Cas to target the AAVS1 locus in human cells

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   Cas9 and gRNA for targeting the AAVS1 locus in human cells
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MBP-Benzonase

   Plasmid

   #218192

   Purpose

   Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001

   Depositor

   Cheryl Arrowsmith

   Insert

   nucA
   

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   April 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3.1-mCherry

    Plasmid

    #128744

    Purpose

    Expresses mCherry in mammalian cells

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-mCherry

    Plasmid

    #114470

    Purpose

    mCherry Control

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBiFC-VC155

    Plasmid

    #22011

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VC155
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Alanine 206 to Lysine

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. hLRP1-pEZT-BM

    Plasmid

    #177985

    Purpose

    Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

    Depositor

    Lora Hooper

    Article

    Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

    Insert

    LDL receptor related protein 1 (LRP1 Human)
    

    Tags

    No

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-puro-cOVA

    Plasmid

    #135073

    Purpose

    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance

    Depositor

    Maria Castro

    Insert

    cOVA (OVAL Chicken)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBiFC-VN173

    Plasmid

    #22010

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VN173
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Venus residus 1-172

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-loxp-DsRed-loxp-eGFP

    Plasmid

    #141148

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    loxp-DsRed-loxp-eGFP
    

    Use

    Lentiviral

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-cMSP1D1

    Plasmid

    #199768

    Purpose

    Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP1D1
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-Lck-mStayGold

    Plasmid

    #234539

    Purpose

    to label the membrane of cells with a fluorescent protein (mStayGold)

    Depositor

    Hajime Hirase

    Article

    Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

    Insert

    Lck-mStayGold(Ando)
    

    Use

    AAV

    Mutation

    None

    Promoter

    Short CAG

    Available Since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-CMV-LoxP-DsRed-LoxP-eGFP

    Plasmid

    #65726

    Purpose

    Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Insert

    LoxP-DsRed-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pYL192 (TRV1)

    Plasmid

    #148968

    Purpose

    Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    TRV RNA1
    

    Use

    Plant vector with trv rna1; this vector used with…

    Mutation

    P697S mutation in putative replicase. S239G and S…

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLAMP1-mCherry

    Plasmid

    #45147

    Depositor

    Amy Palmer

    Article

    Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

    Insert

    LAMP1-mCherry (LAMP1 Human, Synthetic)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)

    Pooled Library

    #1000000049

    Purpose

    CRISPR gRNA knockout pooled library for targeting the human genome

    Depositor

    Feng Zhang

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pYL156 (TRV RNA2)

    Plasmid

    #148969

    Purpose

    Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    Modified TRV RNA2
    

    Use

    T-dna vector

    Mutation

    see comments section below

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. E-cadherin-GFP

    Plasmid

    #28009

    Depositor

    Jennifer Stow

    Article

    Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

    Insert

    E-cadherin (CDH1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. qTAG-C-mNeon-Blast

    Plasmid

    #207709

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYD1-mSA

    Plasmid

    #39865

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin
    

    Tags

    Aga2, FLAG, and HA

    Expression

    Yeast

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-EGFP-Cdc42-T17N

    Plasmid

    #12976

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 DN (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Promoter

    CMV

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Tet-pLKO-neo

    Plasmid

    #21916

    Purpose

    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

    Depositor

    Dmitri Wiederschain

    Article

    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-EGFP-Cdc42-Q61L

    Plasmid

    #12986

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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32. MinLibCas9

    Pooled Library

    #164896

    Purpose

    Human genome-wide CRISPR-Cas9 gRNA library, optimised by mining existing large-scale screening datasets, which provides a >60% reduction in size while preserving key information content

    Depositor

    Mathew Garnett

    Article

    Gonçalves et al Genome Biol. 2021 Jan 21;22(1):40. doi:

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

33. PH-PLCd1-mNGx1

    Plasmid

    #202751

    Purpose

    expresses fluorescent protein-tagged lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    PLCD1v2(1-170):mNG2 (PLCD1 Human)
    

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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34. GoldenBraid 2.0 Kit

    Plasmid Kit

    #1000000076

    Purpose

    Modular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional units

    Depositor

    Diego Orzaez

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

35. MORF library without GFP and mCherry controls

    Pooled Library

    #192821

    Purpose

    Barcoded transcription factor ORF library for high throughput screening

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

36. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid

    #170186

    Purpose

    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

    Depositor

    Bradley Bernstein

    Article

    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

    Insert

    CreERT2-T2A-eBFP2
    

    Use

    Lentiviral

    Promoter

    EF1 alpha

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mOrange2-pBAD

    Plasmid

    #54531

    Purpose

    Localization: Protein Expression Vector , Excitation: 549, Emission: 565

    Depositor

    Roger Tsien, Michael Davidson, Nathan Shaner

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Tags

    6xHis and mOrange2

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AYBEv3

    Plasmid

    #193967

    Purpose

    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    

    Expression

    Mammalian

    Mutation

    G163R, N169S, S198A, K202A, G203A, S206A, K210A

    Promoter

    hU6, EF1a, CBh

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Ef1-a and hU6

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Human SLC overexpression library

    Pooled Library

    #213694

    Purpose

    Inducible barcoded library with 467 codon-optimized cDNAs with UMIs to test the impact of SLC transporters on biological processes.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

41. pAAV-CAG-Lck-mTagBFP2

    Plasmid

    #234537

    Purpose

    to label the membrane of cells with a fluorescent protein (mTagBFP2)

    Depositor

    Hajime Hirase

    Article

    Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

    Insert

    Lck-mTagBFP2
    

    Use

    AAV

    Mutation

    None

    Promoter

    Short CAG

    Available Since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3-myc-FLNa WT

    Plasmid

    #8982

    Depositor

    John Blenis

    Article

    Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

    Insert

    Filamin A (FLNA Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMV-PEmax-SpG

    Plasmid

    #211549

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

    Plasmid

    #71396

    Purpose

    2Gi2R assay

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Inserts

    2xGFP:NES
    2xRFP:NLS
    

    Use

    Lentiviral

    Tags

    IRES

    Expression

    Mammalian

    Promoter

    EF1alpha and IRES

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pDawn

    Plasmid

    #43796

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. hCRISPRa-v2, genome-wide, top 5 sgRNAs/gene

    Pooled Library

    #83978

    Purpose

    Version 2 of the human CRISPRa genome wide library

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Use

    Lentiviral

    Available Since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

47. PGK EGFP

    Plasmid

    #216110

    Purpose

    PGK EGFP

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    PGK promoter
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pICH47772

    Plasmid

    #48004

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197061

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMK344 (Hygro-P2A-mAID)

    Plasmid

    #121178

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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