Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

179,463 results

Showing: 51 - 100 of 179463 results

  1. pAAV.TBG.PI.eGFP.WPRE.bGH (AAV8)

    Viral prep
    #105535-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.TBG.PI.eGFP.WPRE.bGH (#105535). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.eGFP.WPRE.bGH plasmid DNA. TBG-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    TBG
    Tags
    EGFP
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  3. pHR-SFFV-dCas9-BFP-KRAB

    Plasmid
    #46911
    Purpose
    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain
    Depositor
    Stanley Qi, Jonathan Weissman
    Publication
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Insert
    dCas9-BFP-KRAB fusion
    Use
    CRISPR and Lentiviral
    Tags
    2xNLS, BFP, HA, and KRAB domain
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120 Articles
    46911-plasmid-map-sequence-id-449615

    Enlarge

  4. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV5)

    Viral prep
    #44361-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pLVTHM

    Plasmid
    #12247
    Purpose
    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid
    Depositor
    Didier Trono
    Publication
    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194 Articles
    12247-plasmid-map-sequence-id-352425

    Enlarge

  13. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid
    #36047
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag
    Depositor
    Kyle Roux
    Publication
    Roux et al J Cell Biol. 2012 Mar 12.
    Insert
    BirA(R118G)-HA
    Tags
    HA
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Available since
    April 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65 Articles
    36047-plasmid-map-sequence-id-381279

    Enlarge

  17. MCS-BioID2-HA

    Plasmid
    #74224
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68 Articles
    74224-plasmid-map-sequence-id-238509

    Enlarge

  18. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV Retrograde)

    Viral prep
    #105540-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    EGFP
    Available since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-hSyn-DIO-mCherry (AAV8)

    Viral prep
    #50459-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV-CAG-tdTomato (codon diversified) (AAV Retrograde)

    Viral prep
    #59462-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. tdTomato expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available since
    May 10, 2017
    Availability
    Academic Institutions and Nonprofits only

  28. pcDNA3.1 mycBioID

    Plasmid
    #35700
    Purpose
    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
    Depositor
    Kyle Roux
    Publication
    Roux et al J Cell Biol. 2012 Mar 12.
    Insert
    BirA
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    cmv
    Available since
    April 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    94 Articles
    35700-plasmid-map-sequence-id-228311

    Enlarge

  33. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118 Articles
    61427-plasmid-map-sequence-id-322387

    Enlarge

  36. pAAV2/8

    Plasmid
    #112864
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap8
    Use
    AAV
    Promoter
    Truncated P5
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    76 Articles
    112864-plasmid-map-sequence-id-333531

    Enlarge

  40. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Publication
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Promoter
    Tight TRE promoter
    Available since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    82 Articles
    87360-plasmid-map-sequence-id-473508

    Enlarge

  42. pAAV-hSyn-DIO-mCherry (AAV5)

    Viral prep
    #50459-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only

  46. pAAV.TBG.PI.Null.bGH (AAV8)

    Viral prep
    #105536-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.TBG.PI.Null.bGH (#105536). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.Null.bGH plasmid DNA. AAV vector with TBG promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    TBG
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 51 - 100 of 179463 results