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Plasmid#185778PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
RHO3
Bacterial Strain#124700PurposeE. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.DepositorBacterial ResistanceDap (400 µg/ml)Available sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag
Plasmid#132411Purposeoverexpression in human cellsDepositorInsertdRfxCas13b
UseLentiviralTagsflagExpressionMammalianMutationR239A; H244A; R858A; H863APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWSK29
Plasmid#172972PurposeLow copy number cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGK-HALO-CTERM-GFP
Plasmid#185777PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, HaloExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-SMASH-NTERM-GFP
Plasmid#185771PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, SMAShExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR
Plasmid#224435PurposeAAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmScarlet-KASH
U6-gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6b-IVT
Plasmid#215834PurposeTemplate for in vitro transcription of CBE6b (with SpCas9)DepositorInsertCBE6b-SpCas9-2xUGI
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRB_Hormone-recep
Plasmid#109909PurposeProtein expression and purification of ESRRB_Hormone-recepDepositorInsertESRRB_Hormone-recep (ESRRB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby
Plasmid#71760PurposemGFP and Synaptophysin-mRuby expression from Cre-expressing neuronsDepositorHas ServiceAAV1InsertsmGFP
Synaptophysin
UseAAV and Cre/LoxTagsPalmitoylation signal and mRubyExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tet-on circRNA Expression Vector
Plasmid#92351PurposeThe vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.DepositorInsertmCherry and split EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterpTetAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-7xGFP11
Plasmid#138397PurposeRMCE vector for generating C-terminal fusion of seven tandem GFP11 tags (split GFP)DepositorInsert7xGFP11
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3459
Plasmid#144935PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHMGB2 (HMGB2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T2
Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF3286
Plasmid#144762PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR2F6 (NR2F6 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRETightBI-RY-0
Plasmid#31463DepositorInserteYFP and mCherry
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
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AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
Plasmid#65454PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinasesDepositorInsertChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMutationH134R variantPromoterhSyn1Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-3x-p67phox-GBD
Plasmid#214141PurposeRac activity sensorDepositorInsert3xp67phox-GBD (NCF2 Human)
UseTagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-mCherry
Plasmid#80946Purposehuman AAVS1 site targeting donor plasmid for knocking-in mCherry expression cassette driven by CAG promoterDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER Short P2A
Plasmid#164108PurposeDetect the short Mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP Mt-ER Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiP13780: pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780)
Plasmid#214848PurposeExpression of iCre(R297T) in striatal cholinergic neuronsInsertiCre(R297T)
UseAAVTagsExpressionMutationR297TPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mir30
Plasmid#14758DepositorInsertmir30 cassette
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-miniDi-P2A-mCherry
Plasmid#204358PurposeAAV vector for miniDi expression under the control of human synapsin promoterDepositorInsertminiDi-P2A-mCherry
UseAAVTagsmCherryExpressionMutationThe third intracellular loop (ICL3) of hM4Di was …PromoterhSynAvailable sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVG
Plasmid#64084PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein geneDepositorInsertVSV glycoprotein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
PGK-HALO-NTERM-GFP
Plasmid#185772PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, HaloExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-Y1699C
Plasmid#25364DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutationTyrosine 1699 mutated to CysteinePromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR-L858R
Plasmid#116276PurposeLentiviral expression of EGFR L858RDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationL858RPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExp-sfGFP
Plasmid#112573PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis, Strep II, and sfGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only