167,820 results

1. AAVS1-Tet-OsTIR1(F74G)-V5

   Plasmid

   #158664

   Purpose

   Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   Tet-OsTIR1(F74G) AAVS1
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   F74G

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-GFP-SFPQ-IRES-mCherry

   Plasmid

   #166950

   Purpose

   Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   GFP and mCherry

   Promoter

   eF1a

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCk3

   Plasmid

   #136697

   Purpose

   Acceptor plasmid for odd level position 3, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. DDX5 WT V5

   Plasmid

   #199576

   Purpose

   Expresses WT DDX5 cloned into pLenti puro

   Depositor

   Michael Kastan

   Article

   McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

   Insert

   DDX5 (DDX5 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_OTX2_P2A_Hygro_Barcode

   Plasmid

   #120471

   Purpose

   Barcoded lentiviral vector to express OTX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   OTX2 (OTX2 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA GSK3 beta S9A pcDNA3

   Plasmid

   #14754

   Purpose

   Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

   Depositor

   Jim Woodgett

   Article

   Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

   Insert

   GSK3 beta S9A (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S9A. Constitutively active mutant.

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. mCherry-XRCC1

   Plasmid

   #215142

   Purpose

   Expresses mCherry-XRCC1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   XRCC1 (XRCC1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ECFP-Nucleus-7

   Plasmid

   #55346

   Purpose

   Localization: SV40 NLS Triplet, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Insert

   SV40 T-Antigen Nuclear Localization Signal (NLS)
   

   Tags

   ECFP-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAAV-CAG-GFP (AAV6 trial size)

   Viral Prep

   #37825-AAV6.T

   Purpose

   Ready-to-use AAV6 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

10. MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

    Plasmid

    #160369

    Purpose

    Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    GPR143 (GPR143 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet-3xHA

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMF230

    Plasmid

    #62546

    Purpose

    Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

    Depositor

    Michael Franklin

    Article

    Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

    Insert

    GFP mut2
    

    Use

    Broad host range plasmid

    Promoter

    trc

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-Ccdc134-IRES-BFP

    Plasmid

    #117266

    Purpose

    Retroviral overexpression of Ccdc134

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    Ccdc134 (Ccdc134 )
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCk2

    Plasmid

    #136696

    Purpose

    Acceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.

    Depositor

    Nicola Patron

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRRLsinPGK_IRFP720_WPRE

    Plasmid

    #91785

    Purpose

    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    iRFP720
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. N1-G-Ca-FLITS

    Plasmid

    #191465

    Purpose

    Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AiP1795 - pAAV-AiE0170h-minBG-iCre(R297T)-BGHpA

    Plasmid

    #220717

    Purpose

    AiE0170h is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNIC-HIS6-NUSA-TEV-GG

    Plasmid

    #215798

    Purpose

    Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. mCherry-APEX1

    Plasmid

    #215126

    Purpose

    Expresses mCherry-APEX1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    APEX1 (APEX1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBZS-M.CviPI

    Plasmid

    #198355

    Purpose

    Express M.CviPI in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    M.CviPI (M.CviPI )
    

    Expression

    Bacterial

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTK168_mCherry-H2B

    Plasmid

    #46363

    Purpose

    visualizes chromosomes

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    H2B (H2BC11 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D26G, V119I and S125D

    Promoter

    pBABE

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. PCBP1_pLX307

    Plasmid

    #98362

    Purpose

    Lentiviral expression of PCBP1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    PCBP1 (PCBP1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV-HA C/EBP gamma

    Plasmid

    #15715

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    C/EBP gamma (CEBPG Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

23. pHAGE-MET

    Plasmid

    #116762

    Purpose

    Lentiviral expression of MET

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pUltra-Plus-H2B-mTagBFP2

    Plasmid

    #161790

    Purpose

    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Bicistronic

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC and UbC promoter

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. CD47-bio-His

    Plasmid

    #53150

    Purpose

    Expresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD47 (CD47 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pmTurquoise2-H2A

    Plasmid

    #36207

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    H2A (H2AC16 Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCRU5HT1-inLuc

    Plasmid

    #58954

    Purpose

    HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

    Depositor

    Dmitriy Mazurov, David Derse

    Article

    Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

    Insert

    inLuc
    

    Use

    Retroviral

    Promoter

    CMV/R/U5

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pPB_TRE3G_scFV-sfGFP-Ezh2FL

    Plasmid

    #235575

    Purpose

    Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. TFORF3472

    Plasmid

    #144948

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFATC3 (NFATC3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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