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219,539 results

Showing: 10651 - 10700 of 219539 results

  1. v5 ABE-eVLP

    Plasmid
    #228467
    Purpose
    Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs
    Depositor
    David Liu
    Publication
    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag(C507V)–ABE8e
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    MMLVgag(C507V)
    Promoter
    CMV
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    228467-plasmid-map-sequence-id-451030

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  5. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    198397-plasmid-map-sequence-id-389535

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  9. pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE (AAV8)

    Viral prep
    #130991-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE (#130991). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE plasmid DNA. CamKIIa-driven, soma-targeted ChRmine-mScarlet expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie
    Promoter
    CaMKII
    Tags
    mScarlet
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  10. pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde)

    Viral prep
    #162376-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  14. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Publication
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    163148-plasmid-map-sequence-id-331409

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  15. pAAV-hSyn-Cre-P2A-dTomato (AAV2)

    Viral prep
    #107738-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Rylan Larsen
    Promoter
    Syn
    Tags
    dTomato (physically separate, not a fusion protein)
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV PHP.eB)

    Viral prep
    #20298-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  20. GFP-Dcp1a

    Plasmid
    #153972
    Purpose
    Fluorescent protein fused to mRNA decapping enzyme 1a
    Depositor
    Gia Voeltz
    Publication
    Lee et al Science. 2020 Jan 31;367(6477). pii: 367
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRNA decapping enzyme 1a (DCP1A Human)
    Tags
    pAcGFP-C1
    Expression
    Mammalian
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153972-plasmid-map-sequence-id-306138

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  23. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid
    #127368
    Purpose
    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127368-plasmid-map-sequence-id-246345

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  25. pAAV.hSyn-FLEX.iGABASnFR (AAV1)

    Viral prep
    #112163-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn-FLEX.iGABASnFR (#112163). In addition to the viral particles, you will also receive purified pAAV.hSyn-FLEX.iGABASnFR plasmid DNA. Synapsin-driven, Cre-dependent iGABASnFR GABA sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi
    Promoter
    Syn
    Available since
    Jan. 11, 2019
    Availability
    Academic Institutions and Nonprofits only

  29. pKC-4.06

    Plasmid
    #112084
    Purpose
    NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)
    Depositor
    James Inglese
    Publication
    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3XFLAG firefly luciferase-beta-globin 39PTC (HBB Photinus pyralis, Human, Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    112084-plasmid-map-sequence-id-214510

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  32. pGP-AAV-syn-jGCaMP7s-WPRE

    Plasmid
    #104487
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has service
    AAV1, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jGCaMP7s
    Use
    AAV
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42 Articles
    104487-plasmid-map-sequence-id-241275

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  35. pET28a-AviTag-SnoopLigase

    Plasmid
    #105626
    Purpose
    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation
    Depositor
    Mark Howarth
    Publication
    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3
    Insert
    AviTag-SnoopLigase
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    105626-plasmid-map-sequence-id-205296

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  37. pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

    Plasmid
    #103849
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    CMV
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    103849-plasmid-map-sequence-id-199152

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Showing: 10651 - 10700 of 219539 results