190,472 results

1. 2333_Cas9-CtIP-dnRNF168

   Plasmid

   #200254

   Purpose

   This plasmid expresses the Cas9-CtIP-dnRNF168 in mammalian cells

   Depositor

   Claudio Mussolino

   Article

   Carusillo et al Nucleic Acids Res. 2023 May 22;51(9):466

   Insert

   Cas9-CtIP-dnRNF168
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6_N7-sgRNA2

   Plasmid

   #190664

   Purpose

   Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   N7 sgRNA2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDMS151A_Tet-On-3G

   Plasmid

   #191577

   Purpose

   ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

   Depositor

   Joshua Leonard

   Article

   Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

   Insert

   Tet-On(R) 3G
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mTagBFP2-Lifeact-7

   Plasmid

   #54602

   Purpose

   Localization: Actin, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Type

   Empty backbone

   Use

   Tags

   Lifeact and mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLenti.PGK.LifeAct-Ruby.W

   Plasmid

   #51009

   Purpose

   Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

   Depositor

   Rusty Lansford

   Insert

   LifeAct-mRFPruby
   

   Use

   Lentiviral

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   Promoter

   human PGK

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti.PGK.LifeAct-Ruby.W

   Plasmid

   #51009

   Purpose

   Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

   Depositor

   Rusty Lansford

   Insert

   LifeAct-mRFPruby
   

   Use

   Lentiviral

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   Promoter

   human PGK

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-All-in-one-hCascade

   Plasmid

   #134919

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CD68 promoter/enhancer

   Plasmid

   #34837

   Depositor

   Peter Murray

   Article

   Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

   Insert

   CD68 promoter/enhancer -680/+140 (CD68 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-CD68 promoter/enhancer

   Plasmid

   #34837

   Depositor

   Peter Murray

   Article

   Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

   Insert

   CD68 promoter/enhancer -680/+140 (CD68 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV6-Neo-ISG15

    Plasmid

    #80404

    Purpose

    overexpression of ISG15

    Depositor

    Isidoro Martinez

    Article

    Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5-HA-Ubiquitin-K11

    Plasmid

    #22901

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K11 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-HA-Ubiquitin-K11

    Plasmid

    #22901

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K11 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLminP_Luc2P_RE5

    Plasmid

    #90339

    Purpose

    STAT3 - Stat3 homodimer gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT3

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Proinsulin-NanoLuc in pLX304

    Plasmid

    #62057

    Purpose

    Luminescent reporter of insulin secretion

    Depositor

    David Altshuler

    Article

    Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

    Insert

    Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Inserted NanoLuc into C-peptide of mouse Ins2 cod…

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

    Plasmid

    #85428

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-12
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. sfGFP-C1

    Plasmid

    #54579

    Purpose

    Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

    Depositor

    Michael Davidson, Geoffrey Waldo

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. p11-LacY-wtx1

    Plasmid

    #69056

    Purpose

    The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

    Depositor

    Huimin Zhao

    Article

    Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

    Inserts

    ccdB (ccdB )
    lacY (lacY )
    

    Use

    Tags

    A177C

    Expression

    Mutation

    Promoter

    pBAD and pLac

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. ANO6-Plvx-mCherry-c1

    Plasmid

    #62554

    Purpose

    Full length mouse ANO6 tagged with mCherry on the C-terminus

    Depositor

    Renzhi Han

    Article

    Zhao et al PLoS One. 2014 Mar 24;9(3):e92749. doi:

    Insert

    mouse ANO6 (Ano6 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-CAG-Cre

    Plasmid

    #196140

    Purpose

    AAV-mediated expression of Cre under the CAG promoter .

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    codon diversified

    Promoter

    CAG

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EasyClone 2.0 Yeast Toolkit

    Plasmid Kit

    #1000000073

    Purpose

    Integrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strains

    Depositor

    Irina Borodina

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only