167,895 results
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Plasmid#209905PurposeTo monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertEREG-ScNeo (EREG Human)
TagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF2-EFGR-hMer
Plasmid#225996PurposeExpresses a chimeric construct having N-terminal human EGFR and C-terminal human MerTKDepositorExpressionMammalianPromoterEF1-aAvailable SinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS10-ZBASIC-TEV-GG
Plasmid#215797PurposeCloning of target gene with N-terminal His10 Zbasic tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-NoTag-GG
Plasmid#215810PurposeCloning of target gene with no tagsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB-PARP1-del.p119K120S–eGFP
Plasmid#211554PurposeA piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagseGFPExpressionMammalianMutationdeletion of amino acids 119K 120SPromoterCMVAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-PARP1-delM43;F44I
Plasmid#211579PurposeCMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selectionDepositorInsertPARP1 (PARP1 Human)
TagseGFPExpressionMammalianMutationContains deletion of M43 and F44IAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
CHFR-PBZ-eGFP
Plasmid#211581PurposeExpressing CHFR PBZ domain fused to eGFPDepositorInsertCHFR PBZ domain (CHFR Human)
TagseGFPExpressionMammalianMutationPBZ domain of the proteinPromoterCMVAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJR152
Plasmid#196280PurposeCassette used in dual sgRNA library generationDepositorInserthU6-sgRNA-CR3
UseCRISPR and LentiviralPromoterhU6Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL049
Plasmid#198809PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.DepositorInsert15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
ExpressionWormAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal NLS
Plasmid#188398PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterD-mannoseAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-ATG13 (2-197)
Plasmid#188075PurposeTo make ATG101/ATG13 HORMA complex for expression in mammalian cellsDepositorInsertATG13 (ATG13 Human)
Tagsstrep-strep-Flag tagExpressionMammalianMutationATG13 (2-197)PromoterCMVAvailable SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_CCDC124-Halo
Plasmid#175328PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCk4
Plasmid#136698PurposeAcceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.DepositorInsertlacZ
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
RV-mCD4-HH5-4
Plasmid#124984PurposeExpress the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cellsDepositorInsertHH5-4 TCRa P2A TCRb
UseretroviralExpressionMammalianAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PDZK1_PDZ_2
Plasmid#103943PurposeProtein expression and purification of PDZK1 PDZ2 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PDZK1_PDZ_3
Plasmid#103944PurposeProtein expression and purification of PDZK1 PDZ3 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-ATG101-ATG13 HORMA
Plasmid#99332PurposeMammalian expression of one subcomplex from the Human ULK1 complexDepositorAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
EcSTH-LbNOX
Plasmid#211924PurposeThis plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.DepositorInsertEcSTH-LbNOX
UseLentiviralTags3xFLAGAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato
Plasmid#234951PurposeHuman codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmidDepositorInsertHuman codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsFLAGExpressionMammalianPromoterEF1AAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
Plasmid#134868PurposeExpresses EGFP-tagged lysosomal markerDepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseLentiviralTagsGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20_mGFP
Plasmid#190027PurposeDoxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFPDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
araBAD-BCD20-ClpP-BCD17-ClpX
Plasmid#153301PurposeExpresses clpX and clpP in E.ColiDepositorExpressionBacterialPromoteraraBADAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ultra-Exon1Q145-Myc-A
Plasmid#110487PurposeLentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationPartial long 3'UTR wild type huntingtin exon…PromoterUbCAvailable SinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP
Plasmid#113884PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFPDepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepPromoterpHR-CMV-TetO2Available SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_HA1h
Plasmid#190473PurposeExpress the high-affinity histamine sensor GRAB_HA1h in neuronsDepositorInsertHistamine sensor GRAB_HA1h (high affinity)
UseAAVPromoterhSynAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga15
Plasmid#196060PurposeEncodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-VSVG
Plasmid#1733PurposeVSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan labDepositorInsertVSV-G Envelope
ExpressionMammalianAvailable SinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA1m (AAV9)
Viral Prep#140556-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA1m (#140556). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA1m plasmid DNA. Synapsin-driven expression of red fluorescent medium affinity dopamine sensor GRAB_rDA1m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_WT-talin1 (1-2541)
Plasmid#166112PurposeFor expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.DepositorAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
EXT1-pLJC2-3XFLAG
Plasmid#215818PurposeEctopic expression of EXT-1 in mammalian cellsDepositorInsertExostosin glycosyltransferase 1 (EXT1 Human)
UseLentiviralTags3X FlagExpressionMammalianAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only