165,802 results

1. cytoplasmic cpVenus control

   Plasmid

   #186788

   Purpose

   Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   cytoplasmic cpVenus control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-puro-hPPM1A

   Plasmid

   #185382

   Purpose

   For mammalian expression of shRNA: AGGGTAATGGGTTGCGATATG that targets human PPM1A

   Depositor

   Ayaz Najafov

   Insert

   PPM1A (PPM1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

   Plasmid

   #71805

   Purpose

   Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-Tight2-AkaLuc

   Plasmid

   #186186

   Purpose

   AAV vector packaging AkaLuciferase under the TRE promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

   Insert

   AkaLuc luciferase
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE-Tight2

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_mApple-SEPT7 Gmut2

   Plasmid

   #180317

   Purpose

   mammalian expression of human SEPT7 Gmut2 fused to mApple

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN7 (SEPTIN7 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   SEPT7 H279D

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFB2B

   Plasmid

   #195284

   Purpose

   Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   HSV-TK 2A Puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSec61β-mScarlet3-H (aka mYongHong)

   Plasmid

   #239414

   Purpose

   Expresses Sec61b-mScarlet3-H in mammalian cells

   Depositor

   Zhifei Fu

   Article

   Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

   Insert

   Sec61β (SEC61B Human)
   

   Use

   Tags

   mScarlet3-H (aka mYongHong)

   Expression

   Mammalian

   Mutation

   M163H in mScarlet3

   Promoter

   CMV

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY49

   Plasmid

   #128915

   Purpose

   mammalian expression, proximity labelling, mitochondrial relocalisation

   Depositor

   Sean Munro

   Article

   Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

   Insert

   Rab11BSN-BirA*-HA-MAO (RAB11B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-ACVR1C

   Plasmid

   #23615

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ACVR1C (ACVR1C Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pOPINB-OTULIN (full-length, aa 1-352)

    Plasmid

    #61464

    Purpose

    Expresses human OTULIN (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

    Insert

    OTULIN (OTULIN Human)
    

    Use

    Tags

    His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABEpuro HA Brca1

    Plasmid

    #14999

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    Brca1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPagP

    Plasmid

    #112125

    Purpose

    Bacterial expression of outer membrane protein PagP, wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    PagP (pagP E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV_U6-ωRNA*_ISDra2-TnpB-mNG

    Plasmid

    #212968

    Purpose

    AAV vector for encoding an ISDra2-TnpB driven by EFs promoter

    Depositor

    Chunlong Xu

    Article

    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

    Inserts

    ISDra2-TnpB-T2A-mNG,
    ωRNA*
    

    Use

    AAV and CRISPR

    Tags

    NLS and NLS-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, T7 and U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221_SLC5A4

    Plasmid

    #132170

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC5A4 (SLC5A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLPCX mito roGFP2-Orp1

    Plasmid

    #64992

    Purpose

    Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJS61

    Plasmid

    #207585

    Purpose

    GFP-TolA expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TolA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Str-Ii_SBP-mCherry-Golgin84

    Plasmid

    #65304

    Purpose

    synchronize trafficking of Golgin-84 from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. tdTomato-ER-5

    Plasmid

    #58098

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    ER (CALR Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. L-CRISPR-CTN (MLL-ENL)

    Plasmid

    #69212

    Purpose

    Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pN40

    Plasmid

    #128898

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab2AQL-BirA*-HA-MAO (RAB2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV Tmx1-V5

    Plasmid

    #175138

    Purpose

    Lentiviral expression of V5-tagged mouse Tmx1

    Depositor

    Larry Gerace

    Insert

    Tmx1 (Tmx1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-Egr1

    Plasmid

    #11729

    Depositor

    Eileen Adamson

    Article

    Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

    Insert

    Egr-1 (Egr1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRDA_816

    Plasmid

    #201157

    Purpose

    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    5x-ALFA tag-dEnAsCas12a-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D908A

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. qTAG-C-miRFP670nano3-Blast-H3C2

    Plasmid

    #207784

    Purpose

    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pRK5_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

    Plasmid

    #238237

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-2xKS_FtoG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc

    Plasmid

    #193346

    Purpose

    tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    TetO mini CMV

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCCL/IFNB1-d2eGFP-3’UTR

    Plasmid

    #180232

    Purpose

    Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

    Insert

    d2eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    1 kb upstream of IFNB1 CDS

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ES-FUCCI

    Plasmid

    #62451

    Purpose

    SMX_DEST vector with 3 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
    minPGK::Cherry-hCdt1(30-120)-bGHpA
    PGK::HygroR-bGpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTK_Msx1_enh_264_Citrine

    Plasmid

    #130610

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Msx1_enh_264
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MS2-P65-HSF1_GFP

    Plasmid

    #61423

    Purpose

    Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Insert

    MS2(N55K)-P65-HSF1_2A_GFP (HSF1 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LZRS-Mek1-wt1 (wt1)

    Plasmid

    #21196

    Depositor

    Paul Khavari

    Insert

    Mek1 (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

32. pDY1623 NOLC1 sgRNA 2

    Plasmid

    #234834

    Purpose

    sgRNA 2 for NOLC1 STITCHR insertion

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. JB40

    Plasmid

    #128901

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab5ASN-BirA*-HA-MAO (RAB5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHT-trxA.ads

    Plasmid

    #47383

    Purpose

    Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Insert

    ads
    

    Use

    Tags

    6xHis and trxA

    Expression

    Bacterial

    Mutation

    Promoter

    Pgrac

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDONR223_PPARG_WT

    Plasmid

    #82223

    Purpose

    Gateway Donor vector containing PPARG , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PPARG (PPARG Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LeGO-EBFP2

    Plasmid

    #85213

    Purpose

    Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    EBFP2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMC_mNG2(11)_BSDminus_F2

    Plasmid

    #184164

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mEmerald-TPT1-C-10

    Plasmid

    #54283

    Purpose

    Localization: Microtubule Associated Protein, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    TPT1 (TPT1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pTK_Citrine_Nanotag_24_positive_Control

    Plasmid

    #130570

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    FoxD3_cranial_neural_crest_enhancer_NC1
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge