168,004 results

1. Kaede-N1

   Plasmid

   #54726

   Purpose

   Localization: N1 Cloning Vector, Excitation: 508 / 572, Emission: 518 / 580

   Depositor

   Michael Davidson

   Article

   Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

   Type

   Empty backbone

   Tags

   Kaede

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Opie2-PuroR

   Plasmid

   #131613

   Purpose

   Expresses PuroR and dsRed.

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

   Inserts

   PuroR
   dsRed
   

   Expression

   Insect

   Promoter

   Opie2 and hr5Ie1

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBPp65ADZpUw

   Plasmid

   #26234

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   p65AD-Zip (RELA Human)
   

   Expression

   Insect

   Available Since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBad-PhoCl2c

   Plasmid

   #164035

   Purpose

   Improved PhoCl variant with enhanced dissociation efficiency

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl2c
   

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Promoter

   araBad promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTD-NTwinStrep_Km

   Plasmid

   #45938

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Promoter

   Trc (lactose/IPTG inducible)

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Flag-HA-USP38

   Plasmid

   #22580

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP38 (USP38 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GST-AR-624-919

   Plasmid

   #104200

   Purpose

   Expresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion protein

   Depositor

   Elizabeth Wilson

   Article

   He et al Mol Cell Biol. 2003 Mar;23(6):2135-50.

   Insert

   human androgen receptor aa 624-919 (AR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Dec. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Fibronectin-human-plasma in pMAX

   Plasmid

   #120401

   Purpose

   enables eukaryotic expression of human plasma fibronectin

   Depositor

   Inaam Nakchbandi

   Article

   Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

   Insert

   Fibronectin (FN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGTM_COV2_NSP5_004_SUMO

   Plasmid

   #190062

   Purpose

   Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   3CLpro (ORF1ab SARS-CoV-2)
   

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Promoter

   T7 lac

   Available Since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. SLC25A1_pLX307

    Plasmid

    #98374

    Purpose

    Lentiviral expression of SLC25A1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    SLC25A1 (SLC25A1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV1)

    Viral Prep

    #105545-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    HI

    Tags

    EGFP

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pRK5_mEGFP-TAZ-CAMTA1

    Plasmid

    #237642

    Purpose

    For overexpression of mEGFP-TAZ-CAMTA1

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    TAZ-CAMTA1 (WWTR1, CAMTA1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Human Brunello kinome pooled library, guides 1-8 in lentiGuide-Puro backbone

    Pooled Library

    #1000000082

    Purpose

    CRISPR gRNA knockout pooled library for targeting the human kinome

    Depositor

    David Root, John Doench

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

14. ND4-DdCBE-right side TALE

    Plasmid

    #157843

    Purpose

    expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLG005 (Retron-TIR)

    Plasmid

    #157883

    Purpose

    Expresses the retron-TIR defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    Retron-TIR
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available Since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Yes-EGFP

    Plasmid

    #110497

    Purpose

    Fluorescent Yes

    Depositor

    Bernardo Mainou

    Insert

    Yes (YES1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX6p1-HH1713

    Plasmid

    #124994

    Purpose

    E. coli expression of recombinant H. hepaticus antigen HH_1713

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH_1713
    

    Expression

    Bacterial

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC gRNA Shuttle

    Plasmid

    #47024

    Purpose

    Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Insert

    gRNA Shuttle
    

    Use

    CRISPR; Cas9

    Expression

    Plant

    Mutation

    G to T cloning mutation at position 323

    Promoter

    Medicago truncatula U6.6

    Available Since

    Sept. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pQE30-His-NDK

    Plasmid

    #124136

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Nucleotide diphosphate kinase (ndk Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1(+)-Cyto-CaNARi

    Plasmid

    #64849

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Tags

    Cerulean, Nuclear export signal (NES), and Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBS_nanorod_105b

    Plasmid

    #120347

    Purpose

    phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

    Insert

    Staple sequences for a DNA origami nanorod
    

    Expression

    Bacterial

    Promoter

    none

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MS2-epegRNA-Dnmt1

    Plasmid

    #211372

    Purpose

    Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MS2-epegRNA
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGPN-TMPRSS2

    Plasmid

    #187484

    Purpose

    Expression vector - TMPRSS2

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Promoter

    EFS

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFRT_TO_eGFP_TIA1

    Plasmid

    #106094

    Purpose

    gene expression of EGFP-TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIA1 (TIA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn-JEDI-2P-WPRE

    Plasmid

    #179464

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV9

    Insert

    JEDI-2P
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO.1 puro shRNA beta-catenin

    Plasmid

    #18803

    Purpose

    3rd gen lentiviral vector for knocking down beta-catenin gene expression

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    b-catenin shRNA (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3

    Plasmid

    #200630

    Purpose

    Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    iCasp9
    

    Use

    Lentiviral

    Promoter

    pTRE3G

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Talin-CS

    Plasmid

    #83377

    Purpose

    Control FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted at C-terminal o…

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Expression

    Yeast

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBR322-PVCpnf17-22

    Plasmid

    #198272

    Purpose

    Unmodified pPayload; contains WT PVC payloads/regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf17-22
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

    Plasmid

    #209080

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T

    Promoter

    TRE4G

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRRL_U2AF1_WT_mCherry

    Plasmid

    #84017

    Purpose

    expression of U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pQE30-HyPer7

    Plasmid

    #141071

    Purpose

    Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    Hisx6

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUAS-luc2

    Plasmid

    #24343

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Luciferase
    

    Tags

    5XGAL4

    Expression

    Mammalian

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. His6TEV-UbG76C

    Plasmid

    #75299

    Purpose

    Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    human Ubiquitin mutant G76 to C
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Glycine 76 to Cystine

    Promoter

    T7

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pIVM_02

    Plasmid

    #204500

    Purpose

    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    amino acids 54-213 VHL (VHL Human)
    

    Tags

    6xHis, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    0

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLVX-Puro-hGSDME

    Plasmid

    #154876

    Purpose

    Expresses hGSDME in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. STIM1-EGFP

    Plasmid

    #210370

    Purpose

    Expresses STIM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge