We narrowed to 15,977 results for: LEA

1. SBE-NLS-mCherry PGK-rtTA3

   Plasmid

   #195332

   Purpose

   Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Inserts

   NLS-mCherry
   rtTA3
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   hPGK and miniCMV

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAR-Ec633

   Plasmid

   #130873

   Purpose

   A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

   Depositor

   Chang Liu

   Article

   Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

   Insert

   TP-DNAP1
   

   Expression

   Yeast

   Mutation

   L477V, L640Y, I777K, W814N

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. H2B-2A-GFP-IRESpuro2

   Plasmid

   #183746

   Purpose

   Expresses H2B-2A-GFP

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Tags

   GFP with 2A protease cleavage site between H2B an…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SENP2M497A

   Plasmid

   #61088

   Purpose

   cleave the SUMO off when use PETSUMO vector for express fusion protein

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

   Insert

   SENP2M497A catalytic domain (SENP2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   M497A; Catalytic domain is residues 364-589

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

5. AAV-syn-ChR2-EYFP-Kv

   Plasmid

   #89256

   Purpose

   Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

   Depositor

   McLean Bolton

   Article

   Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

   Insert

   Channelrhodopsin
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   synapsin

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3.1-H-Luc_nucl

   Plasmid

   #113931

   Purpose

   Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc_nucl
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

   Plasmid

   #228364

   Purpose

   mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Enascas12a nuclease and en…

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLENTI_RPS10

   Plasmid

   #127129

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   RPS10 (RPS10 Human)
   

   Use

   Lentiviral

   Mutation

   K24R- please see depositor comments

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDC191

   Plasmid

   #115792

   Purpose

   Expresses Rat synaptobrevin 2

   Depositor

   Axel Brunger

   Article

   Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

   Insert

   Synaptobrevin2 (Vamp2 Rat)
   

   Tags

   hexa histidine

   Expression

   Bacterial

   Mutation

   S28C and C103S (please see comments below)

   Promoter

   T7

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMF-TetTight

    Plasmid

    #193288

    Purpose

    Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

    Depositor

    Rudolf Jaenisch

    Article

    Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

    Insert

    rtTA-P2A-tTS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF‐1α

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTC217

    Plasmid

    #70018

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CAG-H2B-GFP

    Plasmid

    #116869

    Purpose

    Nuclear localized GFP

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    GFP
    

    Use

    AAV

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mTurquoise2-LaminB

    Plasmid

    #98830

    Purpose

    In vivo visualization of the nuclear envelope (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    LaminB
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET15b PKA Cat

    Plasmid

    #14921

    Depositor

    Susan Taylor

    Article

    Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

    Insert

    PKA catalytic subunit alpha (Prkaca Mouse)
    

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    July 24, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-FKBP-Venus-173-C

    Plasmid

    #87617

    Purpose

    FKBP-tagged C-terminal Venus (YFP) fragment for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    FKBP-Venus173-C
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-SpyTag002-MBP

    Plasmid

    #102831

    Purpose

    Bacterial expression of SpyTag002 fused to maltose binding protein

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyTag002-MBP
    

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to the N-terminus of MBP via a …

    Promoter

    T7

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGSKU

    Plasmid

    #72243

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    kanMX4
    I-SceI
    

    Expression

    Bacterial

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-YU2gp160

    Plasmid

    #100927

    Purpose

    Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (YU2) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCSC-GFP-NES-EGFP

    Plasmid

    #221810

    Purpose

    Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    GFP-NES-EGFP
    

    Use

    Lentiviral

    Tags

    NES (internal tag)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTC223

    Plasmid

    #70019

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMB1: All in one SpCas9 expression and sgRNA cloning backbone

    Plasmid

    #228363

    Purpose

    mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-6His-MBP-MpAgo

    Plasmid

    #79249

    Purpose

    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

    Depositor

    Jennifer Doudna

    Article

    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

    Insert

    Marinitoga piezophila Argonaute
    

    Use

    CRISPR

    Tags

    6XHis-tag + MBP + PreScission Cleavage Site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNCS mRFP

    Plasmid

    #91765

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

    Insert

    monomeric Red Fluorescent Protein1
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCEP4-BaLgp160

    Plasmid

    #100919

    Purpose

    Mammalian expression plasmid for Env from the BaL HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRRL_U2AF1_S34F_mCherry

    Plasmid

    #84019

    Purpose

    expression of mutant U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    S34F

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCoofy57

    Plasmid

    #122009

    Purpose

    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His8 and Multi1: depending on the LP2 primer, eit…

    Expression

    Mammalian

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTP1174

    Plasmid

    #104162

    Purpose

    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Flag-NRF1-HA

    Plasmid

    #34708

    Depositor

    Raymond Deshaies

    Article

    Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

    Insert

    NRF1 (NFE2L1 Human)
    

    Use

    Retroviral

    Tags

    3XFlag and HA

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

    Plasmid

    #175524

    Purpose

    Co-expression of HaloTag7 (golgi) and SNAPf-tag (membrane inner leaflet) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7 and Lyn11-SNAPf
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Flag-NRF1

    Plasmid

    #34707

    Depositor

    Raymond Deshaies

    Article

    Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

    Insert

    NRF1 (NFE2L1 Human)
    

    Use

    Retroviral

    Tags

    3XFlag

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

32. 14UAS PSD95:GFP 5UAS DSRedExpress

    Plasmid

    #74315

    Purpose

    Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

    Depositor

    Stephen Smith, Martin Meyer

    Article

    Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

    Inserts

    zebrafish PSD95
    DsRedExpress
    

    Tags

    EGFP

    Mutation

    Y599H and I793S (please see depositor comment bel…

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pAR-Ec611

    Plasmid

    #130872

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Expression

    Yeast

    Mutation

    I777K, L900S

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Orai1 CFP

    Plasmid

    #19757

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCEP4-DU422gp160

    Plasmid

    #100926

    Purpose

    Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (DU422.1) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCEP4-CH111gp160

    Plasmid

    #123268

    Purpose

    Mammalian expression plasmid for Env from the CH111 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCEP4-B41gp160

    Plasmid

    #123247

    Purpose

    Mammalian expression plasmid for Env from the B41 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (B41) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-Venus-CAAX

    Plasmid

    #87612

    Purpose

    Plasma membrane-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus-CAAX
    

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. cr3Dd

    Plasmid

    #184862

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, bet, exo
    

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCEP4-AD8gp160

    Plasmid

    #123260

    Purpose

    Mammalian expression plasmid for Env from the AD8 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCEP4-25711gp160

    Plasmid

    #111841

    Purpose

    Mammalian expression plasmid for Env from the 25711 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (25711.2) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCEP4-CH115gp160

    Plasmid

    #123280

    Purpose

    Mammalian expression plasmid for Env from the CH115 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (CH115) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCEP4-001428gp160

    Plasmid

    #123255

    Purpose

    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (001428) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCEP4-H031gp160

    Plasmid

    #123272

    Purpose

    Mammalian expression plasmid for Env from the H031 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (H031) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. JDW 1039 (pLenti6.2_CMV_V5_TCF7_P2A_H2A_mCherry)

    Plasmid

    #233539

    Purpose

    A CMV driven human TCF7 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    TCF7 (TCF7 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. JDW 1045 (pLenti6.2_CMV_V5_TCF7L2_P2A_H2A_mCherry)

    Plasmid

    #233541

    Purpose

    A CMV driven human TCF7L2 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PP1 7-300

    Plasmid

    #26566

    Depositor

    Wolfgang Peti

    Article

    Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

    Insert

    PP1a (PPP1CA Human)
    

    Tags

    His6, TEV cleavage site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains only aa 7-330

    Available Since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

48. P2-ECFP-pA (Construct 6)

    Plasmid

    #55198

    Purpose

    Synthetic promoter responsive to gRNA2

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    Minimal Ade MLP

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3-smURFP-StagRFP-EKAR-EV

    Plasmid

    #173019

    Purpose

    Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    smURFP-StagRFP-EKAR-EV
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pGSHU

    Plasmid

    #72244

    Purpose

    Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    hyg
    I-SceI
    

    Expression

    Bacterial

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge