164,868 results

1. pAAV-TRE-mTagBFP2-WPRE

   Plasmid

   #193339

   Purpose

   AAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   mTagBFP2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   A XhoI site in the coding sequence is silenced

   Promoter

   TRE

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMV306hsp+LuxG13

   Plasmid

   #26161

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   Bacterial luciferase operon + G13 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-ChroME2f-mRuby2-ST

   Plasmid

   #170160

   Purpose

   Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2f-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-U6+27-Tornado-RhoBAST

   Plasmid

   #196341

   Purpose

   Overexpression of circular RhoBAST

   Depositor

   Murat Sunbul

   Article

   Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

   Insert

   Tornado-RhoBAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ERmoxGFP

   Plasmid

   #68072

   Purpose

   mammalian expression of ER localized moxGFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Insert

   moxGFP
   

   Use

   Tags

   KDEL ER retention sequence and bovine prolactin s…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFW6000

   Plasmid

   #224215

   Purpose

   Bacteroides genomic editing tool; aTc inducible-Fncpf1

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Insert

   Fncpf1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0056

   Plasmid

   #142529

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MAF (MAF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pnEA-vH_His-TEV-SEPT2

   Plasmid

   #174491

   Purpose

   bacterial expression of human SEPT2

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cherry-Fibrillarin-C1

   Plasmid

   #134539

   Purpose

   Expresses Cherry-Fibrillarin fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   Cherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. JD630

    Plasmid

    #160394

    Purpose

    Vitis GRF4-GIF1 in pDONR-zeo

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    Vitis GRF4-GIF1 chimera
    

    Use

    Entry clon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. EFSp-GFP-Empty

    Plasmid

    #174171

    Purpose

    Control lentiviral vector expressing GFP under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDONR223_SUV39H1_WT

    Plasmid

    #82236

    Purpose

    Gateway Donor vector containing SUV39H1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SUV39H1 (SUV39H1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-NFAT1(4-460)-GFP

    Plasmid

    #11107

    Purpose

    Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

    Depositor

    Anjana Rao

    Article

    Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Tags

    3x HA and GFP

    Expression

    Mammalian

    Mutation

    Contains amino acids 4-460 of NFAT1; L78P compare…

    Promoter

    CMV

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70

    Plasmid

    #62904

    Purpose

    Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4DBD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xHA-TurboID pRetrox

    Plasmid

    #155203

    Purpose

    For doxycycline-inducible expression of 3xHA-TurboID on N-terminus of bait protein

    Depositor

    Kyle Roux

    Article

    May et al Cells. 2020 Apr 25;9(5). pii: cells90510

    Insert

    3xHA-TurboID
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promotor

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

    Plasmid

    #61590

    Purpose

    Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

    Depositor

    David Komander

    Article

    Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

    Insert

    TRABID (ZRANB1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-244 and aa 698-708.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSP1E1

    Plasmid

    #20062

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E1 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeat of hel…

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBa. BDNF-Halo

    Plasmid

    #162718

    Purpose

    Mammalian expression of rat BDNF

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Brain derived neurotrophic factor (Bdnf Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. EMTB-mCherry

    Plasmid

    #26742

    Depositor

    William Bement

    Article

    Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Probe for microtubules

    Tags

    2x mCherry and Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV Retrograde)

    Viral Prep

    #105551-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CaMKII

    Tags

    GFP

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

21. lenti F9-TetR-EGFP-IRES-PuroR

    Plasmid

    #117049

    Purpose

    lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Insert

    F9-TetR-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    F9 promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pWZL-Blast-Flag-HA-hTERT

    Plasmid

    #22396

    Depositor

    William Hahn

    Article

    Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

    Insert

    TERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. paGFP-Omp25

    Plasmid

    #69598

    Purpose

    expresses GFP-labeled mitochondrial outer membrane protein 25

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    paGFP-Omp25 (Synj2bp Rat)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    photoactivatable GFP fused to rat Omp25 C-terminus

    Promoter

    CMV

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. Lifeact-Halo

    Plasmid

    #193328

    Purpose

    mammalian transient expression - expression of Lifeact-Halo

    Depositor

    Klaus Hahn

    Article

    Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

    Insert

    Lifeact
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid

    #78311

    Purpose

    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

    Depositor

    Timo Otonkoski

    Article

    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

    Insert

    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA4TO-24xGCN4_v4-BFP-24xPP7

    Plasmid

    #74929

    Purpose

    Translation reporter

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    24xGCN4_v4-BFP-24xPP7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV-CAG-jGCaMP8s-WPRE

    Plasmid

    #179256

    Purpose

    AAV-mediated expression of GCaMP8s under the CAG promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pECE-M2-CDC27 wt

    Plasmid

    #31661

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    cell division cycle 27 homolog (CDC27 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

29. mCRISPRi-v2, Gene Expression (m5), top 5 sgRNAs/gene

    Pooled Library

    #83993

    Purpose

    Mouse CRISPRi Pooled Library targeting gene expression

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

30. p6659 MSCV-IP N FlagHA HPV16E6

    Plasmid

    #44152

    Depositor

    Peter Howley

    Article

    White et al J Virol. 2012 Dec;86(24):13174-86. doi:

    Insert

    HPV16E6
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

31. PyronicSF/pcDNA3.1(-)

    Plasmid

    #124812

    Purpose

    Highly resposive GFP-based pyruvate nanosensor.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Human Kinase Domain-Focused CRISPR Knockout Library

    Pooled Library

    #117725

    Purpose

    gRNAs targeting the kinase domain of human protein kinases

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

33. pBAD-bARGSer-AxeTxe

    Plasmid

    #192473

    Purpose

    Second-generation bacterial acoustic reporter gene derived from Serratia

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Inserts

    bARGSer
    AxeTxe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    the gene Ser39006_001280 was deleted from the clu…

    Promoter

    Native promoter from Enterococcus faecium and pBAD

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-Ef1a-DIO mScarlet (AAV5)

    Viral Prep

    #131002-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO mScarlet (#131002). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO mScarlet plasmid DNA. EF1a-driven, Cre-dependent mScarlet expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    EF1a

    Tags

    mScarlet (Cre-dependent)

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

35. pMXs-beta-catenin-S33Y

    Plasmid

    #13371

    Purpose

    Retroviral expression of mouse beta-catenin S33Y mutant

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    catenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Serine 33 to Tyrosine and Leucine 388 to Proline

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti Lifeact-iRFP670 BlastR

    Plasmid

    #84385

    Purpose

    Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

    Plasmid

    #20943

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available since

    July 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKOR1

    Plasmid

    #133446

    Purpose

    Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

    Depositor

    Olaf Schneewind, Taeok Bae

    Article

    Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

    Type

    Empty backbone

    Use

    E.coli/s.aureus shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET15b-hnRNP-H

    Plasmid

    #23020

    Depositor

    Douglas Black

    Article

    Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

    Insert

    hnRNP-H (HNRNPH1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA-LoxP-mKate-LoxP-EGFP

    Plasmid

    #167940

    Purpose

    CMV promoter-LoxP-mKate-LoxP-EGFP

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    LoxP-mKate-LoxP-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge