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Showing: 10201 - 10240 of 160933 results

  2. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV5)

    Viral Prep
    #26975-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. pSBtet-BH

    Plasmid
    #60499
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60499-plasmid-map-sequence-id-97548

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  7. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73440-plasmid-map-sequence-id-135339

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  21. R777-E283 Hs.ROCK1

    Plasmid
    #70567
    Purpose
    Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK1 (ROCK1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70567-plasmid-map-sequence-id-126016

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  24. pHes7-Achilles-Hes7

    Plasmid
    #153528
    Purpose
    Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.
    Depositor
    Ryoichiro Kageyama
    Article
    Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:
    Insert
    Hes7 (Hes7 Mouse)
    Use
    Tags
    Achilles
    Expression
    Mammalian
    Mutation
    Promoter
    mouse Hes7 promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153528-plasmid-map-sequence-id-305297

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  26. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83476-plasmid-map-sequence-id-189195

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  27. BK Dunlop

    Plasmid
    #25466
    Depositor
    Peter Howley
    Insert
    human papovavirus BK Dunlop
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 26, 2011
    Availability
    Academic Institutions and Nonprofits only

  28. pAAV-Ef1a-Con/Fon-BFP (AAV8)

    Viral Prep
    #137129-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-BFP (#137129). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-BFP plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of BFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    BFP (Cre and Flp-dependent)
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only

  31. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208942-plasmid-map-sequence-id-415667

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  33. TFORF1497

    Plasmid
    #143126
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LYL1 (LYL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143126-plasmid-map-sequence-id-397859

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  36. hSyn1-eYFP (AAV Retrograde)

    Viral Prep
    #117382-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Promoter
    Syn
    Tags
    eYFP
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  37. TFORF2535

    Plasmid
    #142956
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX6 (SOX6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142956-plasmid-map-sequence-id-397292

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Showing: 10201 - 10240 of 160933 results