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Showing: 521 - 540 of 33667 results

  8. OfCasp3b

    Plasmid
    #166054
    Purpose
    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Orbicella faveolata caspase-3b
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166054-plasmid-map-sequence-id-328273

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  9. OfCasp3a

    Plasmid
    #166053
    Purpose
    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Orbicella faveolata caspase-3a
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166053-plasmid-map-sequence-id-328272

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  10. PaCasp3

    Plasmid
    #166052
    Purpose
    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Porites astreiodes caspase-3
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166052-plasmid-map-sequence-id-328270

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  11. PaCasp7a

    Plasmid
    #166051
    Purpose
    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Porites astreiodes caspase-7a
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166051-plasmid-map-sequence-id-328269

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  15. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

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  16. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  17. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid
    #136656
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    136656-plasmid-map-sequence-id-275491

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  18. B-HypaSpCas9

    Plasmid
    #126763
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A; M694A; Q695A; H698A; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126763-plasmid-map-sequence-id-410968

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  19. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid
    #136655
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136655-plasmid-map-sequence-id-273341

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  20. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid
    #136653
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136653-plasmid-map-sequence-id-273329

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Showing: 521 - 540 of 33667 results