196,403 results

1. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

   Plasmid

   #55222

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAJ075_hsCRBN

   Plasmid

   #124214

   Purpose

   Insect cell expression vector for His6 tagged hsCRBN

   Depositor

   Eric Fischer

   Article

   Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

   Insert

   Cereblon (CRBN Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-Matr3 delRRM1

   Plasmid

   #32881

   Depositor

   Yossi Shiloh

   Article

   Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

   Insert

   MATR3 Matrin3 (MATR3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   deleted amino acids 399-468

   Promoter

   CMV

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pGOAL17

   Plasmid

   #20189

   Depositor

   Tanya Parish

   Article

   Parish et al Microbiology. 2000 Aug . 146 ( Pt 8)():1

   Type

   Empty backbone

   Use

   Marker cassette

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Syn-VARNAM2

   Plasmid

   #195520

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   Synapsin

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1 F-tractin-EGFP

   Plasmid

   #58473

   Purpose

   Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   F-tractin (Itpka Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tet-O-FUW-EGFP

   Plasmid

   #30130

   Depositor

   Marius Wernig

   Article

   Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

   Insert

   Enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. LBR EGFP

   Plasmid

   #128150

   Purpose

   Expression of lamin B receptor

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

   Insert

   lamin B receptor (LBR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TREM2_Halo_N_allele

   Plasmid

   #178122

   Purpose

   Donor vector for endogenous tagging of human TREM2 at the N-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (TREM2 Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

    Plasmid

    #64613

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S33A, S37A, T41A, S45A

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. MIC60-mCherry

    Plasmid

    #232789

    Purpose

    Encodes an mCherry-tagged MIC60/IMMT

    Depositor

    Giulia Bertolin

    Article

    Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

    Insert

    MICOS complex subunit MIC60 (IMMT Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-P2X2-YFP

    Plasmid

    #22400

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSEVA351-Cpf1

    Plasmid

    #192361

    Purpose

    CRISPR-based vector for genome editing

    Depositor

    Juana María Navarro Llorens

    Article

    Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

    Insert

    Cas12a, gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. V5-APEX2-SENP2

    Plasmid

    #129276

    Purpose

    Expression of APEX2 at nuclear pore

    Depositor

    Alice Ting

    Article

    Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

    Insert

    V5-APEX2-SENP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cdk1-mCherry

    Plasmid

    #54466

    Purpose

    CDK1-mCherry in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

    Insert

    Cdk1 mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF3081

    Plasmid

    #144557

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELK3 (ELK3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-PIK3C2B

    Plasmid

    #161989

    Purpose

    Mammalian expression vector containing PIK3C2B tagged with GFP

    Depositor

    Volker Haucke, David Bryant

    Article

    Marat et al Science. 2017 Jun 2;356(6341):968-972. d

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    siRNA resistance to siRNA#2 with 4 silent mutatio…

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Nme2Cas9_pMCSG7

    Plasmid

    #119925

    Purpose

    Nme2Cas9 bacterial expression plasmid

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    Tags

    NLS, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. 39R861+

    Bacterial Strain

    #119737

    Purpose

    Six stable plasmids can be used as size standards

    Depositor

    Ruth Hall

    Article

    Moran et al Plasmid. 2019 Mar;102:6-9. doi: 10.1016/

    Bacterial Resistance

    None

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-S5E2-GCaMP6f (AAV1)

    Viral Prep

    #135632-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Promoter

    Tags

    None

    Available since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

21. pAAV-hsyn-GRAB_DA-mut (AAV9)

    Viral Prep

    #140555-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA-mut (#140555). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA-mut plasmid DNA. Synapsin-driven expression of control dopamine sensor GRAB_DA-mut (does not respond to DA). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Promoter

    Syn

    Tags

    None

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

22. pT4-U6-sgRNA-CMV-EGFP

    Plasmid

    #239587

    Purpose

    Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Type

    Empty backbone

    Use

    Sleeping beauty transposone

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. PKC alpha WT

    Plasmid

    #21232

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC alpha (PRKCA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lentiCRISPRv2_ISG

    Pooled Library

    #125753

    Purpose

    Expresses gRNAs targeting human Interferon-Stimulated Genes

    Depositor

    Molly OhAinle, Michael Emerman

    Article

    Roesch et al Retrovirology. 2018 Mar 20;15(1):26. doi

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

25. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. qTAG-N-Blast-miRFP670nano3

    Plasmid

    #207680

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. repressilator (pZS1-lTlrLLtCL)

    Plasmid

    #26489

    Depositor

    Michael Elowitz

    Article

    Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

    Insert

    LacI, tetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. iMbody_pSANG10-3F

    Plasmid

    #184496

    Purpose

    Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

    Depositor

    Marcel Dinger

    Article

    Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

    Insert

    iMbody
    

    Use

    Tags

    3xFLAG, 6xHis, and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid

    #218628

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    EcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDH_CMV_TFAM_mKate2

    Plasmid

    #167331

    Purpose

    Expresses mKate2 fused to full length TFAM in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHR-tdPP7-3xmCherry

    Plasmid

    #74926

    Purpose

    Expresses tdPP7-3xmCherry

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    PP7-3xmCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV_hSyn-PdCO-EGFP-WPRE (AAV1)

    Viral Prep

    #198513-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV_hSyn-PdCO-EGFP-WPRE (#198513). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-EGFP-WPRE plasmid DNA. Synapsin-driven expression of the optimized opsin PdCO in frame with EGFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Promoter

    Syn

    Tags

    EGFP

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

34. pLminP_Luc2P_RE3

    Plasmid

    #90337

    Purpose

    AHR - Aryl hydrocarbon receptor gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    AHR

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV-PB-SRT-tdTomato

    Plasmid

    #154889

    Purpose

    piggyBac self-reporting transposon with tdTomato marker in AAV backbone

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    piggyBac SRT
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PRS303-240xtetO

    Plasmid

    #44754

    Depositor

    Narendra Maheshri

    Article

    Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

    Insert

    240x tetO array and CHRI homology
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42337

    Purpose

    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. His-CL7-2NLS-SpyCas9(dS)-A22p3star

    Plasmid

    #222970

    Purpose

    Bacterial expression of A22p-fused SpyCas9 construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His-CL7-2NLS-SpyCas9(dS)-A22p3
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCEP4-M2L

    Plasmid

    #20926

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Inserts

    MYC (MYC Human)
    LIN28 (LIN28A Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    CMV and IRES

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTA7002-avrPto

    Plasmid

    #49156

    Purpose

    Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto

    Depositor

    Sheng Yang He

    Article

    Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

    Insert

    avrPto
    

    Use

    Plant transformation binary vector

    Tags

    Expression

    Mutation

    Promoter

    dexamethasone inducible

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only