167,785 results

1. KW54_HART_ODC1

   Plasmid

   #207398

   Purpose

   Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR

   Depositor

   Stephen Floor

   Article

   Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

   Insert

   eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ucp1-Cre-miR122

   Plasmid

   #228408

   Purpose

   Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

   Depositor

   Joerg Heeren

   Article

   Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

   Inserts

   cre recombinase with SV40 NLS
   Cre recombinase with SV40 NLS
   

   Use

   AAV

   Promoter

   rat Ucp1 enhancer-promoter

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSP2-48-merTetO-EFS-BLaR

   Plasmid

   #118712

   Purpose

   Contains an optimized 48-mer TetO repeat for imaging of desired loci

   Depositor

   Huimin Zhao

   Article

   Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

   Inserts

   Blasticidin resistance gene
   EFS-NS promoter
   

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKCyR8

   Plasmid

   #149494

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CymR(AM)-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PCymRC

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. BLBP-mCherry

   Plasmid

   #63721

   Purpose

   Expressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

   Depositor

   Bart De Strooper

   Article

   Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

   Insert

   BLBP (Fabp7 Mouse)
   

   Expression

   Mammalian

   Mutation

   1700 bp upstream of BLBP gene cloned in CAG-mCher…

   Promoter

   1700bp upstream of BLBP gene

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK289 (mAID-mClover-NeoR)

   Plasmid

   #72827

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Neo
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pplss-mRFP-KDEL-pcDNA3.1/Hygro

   Plasmid

   #242808

   Purpose

   ER marker

   Depositor

   Christoph Kaether

   Insert

   mRFP with ER targeting signal sequence and C-terminal KDEL-ER retention signal
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV2 Bxb1-c22

   Plasmid

   #242874

   Purpose

   Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, A369P, E434G)

   Depositor

   Jesse Owens

   Article

   Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

   Insert

   Bxb1 Integrase
   

   Expression

   Mammalian

   Mutation

   I87L, A369P, E434G

   Promoter

   CMV

   Available Since

   Sept. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. SIN40C.SFFV.dCas9-KRAB.P2A.mNeon

    Plasmid

    #170482

    Purpose

    Lentiviral dSpCas9-KRAB (CRISPRi) delivery, mNeon coexpression

    Depositor

    Dirk Heckl

    Article

    Ng et al iScience. 2023 Sep 7;26(10):107844. doi:

    Inserts

    dCas9-KRAB
    SFFV
    P2A-mNeonGreen
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF1-Pls4-sfGFP

    Plasmid

    #205751

    Purpose

    Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.

    Depositor

    Jerome Bonnet

    Article

    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

    Type

    Empty backbone

    Use

    Shuttle vector gram+ gram-

    Expression

    Bacterial

    Promoter

    Pls4 (Phyperspank mutant, IPTG inducible)

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE2-Ef1a-SOX2-3xFLAG

    Plasmid

    #216197

    Purpose

    Overexpress human SOX2 in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUW mCherry-P2A-NeoR

    Plasmid

    #239570

    Purpose

    Cytosolic expression of mCherry

    Depositor

    Tim Ahfeldt, Joel Blanchard

    Article

    et al iScience 28 (2025)

    Insert

    mCherry
    

    Use

    Lentiviral

    Promoter

    UbC

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRE2_CMV_Mex3a_t2a_mCherry

    Plasmid

    #223244

    Purpose

    tetO responsive expression of Mex3a in mammalian cells with t2a cleaved mCherry

    Depositor

    Stavros Lomvardas

    Insert

    Mex3a (Mex3a Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    amino acids conserved, underlying DNA sequence re…

    Promoter

    CMV

    Available Since

    May 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV_Mex3a_mutRING_t2a_mCherry

    Plasmid

    #223245

    Purpose

    Expression of mutant RING Mex3a in mammalian cells with t2a cleaved mCherry

    Depositor

    Stavros Lomvardas

    Insert

    Mex3a (Mex3a Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    4 point mutations in RING Domain: Cys 484 Ala; Hi…

    Promoter

    CMV

    Available Since

    May 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKEK3230

    Plasmid

    #234102

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    FuGFP
    

    Expression

    Bacterial

    Promoter

    pJ23100-BsaI

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJPC259

    Plasmid

    #234531

    Purpose

    Cre/Lox 71/66 Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Expression

    Plant

    Mutation

    none

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJPC055

    Plasmid

    #234536

    Purpose

    TRV2 expressing Cre

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    Cre recombinase
    

    Use

    Recombinase

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. SRP19 in pLX311

    Plasmid

    #233292

    Purpose

    Expression of WT SRP19

    Depositor

    Sefi Rosenbluh

    Article

    Xi et al Proc Natl Acad Sci U S A. 2025 Apr 15;12

    Insert

    SRP19 (SRP19 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHW1385

    Plasmid

    #229879

    Purpose

    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    

    Tags

    mScarlet-I-C1

    Expression

    Worm

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. PKMC356_pTRE-ActivinA_IRES_BFP_WPRE

    Plasmid

    #225355

    Purpose

    Lentiviral vector for ActivinA and BFP expression

    Depositor

    Wendell Lim

    Article

    Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

    Insert

    Actvin A and TagBFP (Inhba Synthetic, Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHPHS853

    Plasmid

    #228236

    Purpose

    Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible EYFP reporter for integration into Bxb1 attP_GA landing pad

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

    Inserts

    mCherry
    EYFP
    

    Tags

    HA and PuroR

    Expression

    Mammalian

    Promoter

    TRE3GV and none

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pHPHS309

    Plasmid

    #228230

    Purpose

    GFP dropout backbone for sgRNA library cloning

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. qTAG-N-Solo-mStayGold-ACTB

    Plasmid

    #227326

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid

    #227321

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. qTAG-C-mScarlet-EFS-Zeo

    Plasmid

    #227261

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FFA2-DuET

    Plasmid

    #213238

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    FFA2 (FFAR2 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

    Plasmid

    #210709

    Purpose

    This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    U6

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBad33_IS605_reRNA

    Plasmid

    #205321

    Purpose

    Bacterial expression of IS605 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    IS605 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLV-Kif5b-T92N-Myc

    Plasmid

    #186615

    Purpose

    Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B Rigor mutant (T92N) fused to Myc tag

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Kinesin Heavy Chain isoform 5b Rigor mutant (T92N) (KIF5B Human)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    T92N

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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31. pJEP201-Lenti-TRE3G-Flag-GluN2B-WPRE-pA

    Plasmid

    #164775

    Purpose

    pLenti vector backbone designed to express Flag-GluN2B from a TRE3G promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2B
    

    Use

    Lentiviral

    Tags

    Flag

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a-XPO5-His6

    Plasmid

    #171562

    Purpose

    Encodes human XPO5 protein

    Depositor

    Yan Zeng

    Insert

    XPO5 (XPO5 Human)
    

    Expression

    Bacterial

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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33. pGA2ox2:GUS

    Plasmid

    #108564

    Purpose

    Arabidopsis transformation, expresses GA2ox2 promoter/GUS fusion to study expression pattern in roots

    Depositor

    Ton Bisseling

    Article

    Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

    Insert

    GA2ox2 promoter (GA2OX2 Mustard Weed)
    

    Tags

    GUS

    Expression

    Plant

    Promoter

    GA2ox2

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA-ET-F656W

    Plasmid

    #72546

    Purpose

    expresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    BRD4 ET (BRD4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F656W

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA-ET-I654Q

    Plasmid

    #72545

    Purpose

    expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    BRD4 ET (BRD4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I654Q

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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36. pcDNA-ET-L630W

    Plasmid

    #72543

    Purpose

    expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    BRD4 ET (BRD4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L630W

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGL4.R_TRE_minCMV

    Plasmid

    #211518

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    SV40 and minCMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Expression

    Worm

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV8)

    Viral Prep

    #105547-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    EF1a

    Tags

    EGFP

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

40. pLV-TetO-hNGN2-Neo

    Plasmid

    #99378

    Purpose

    Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    hNGN2-P2A-Neo (NEUROG2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge