215,785 results

1. pME phluorin2-gpi

   Plasmid

   #73793

   Purpose

   ratiometric pHluorin2 with a gpi linkage sequence in a middle entry clone

   Depositor

   David Raible

   Article

   Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

   Insert

   ratiometric pHluorin2-gpi
   

   Use

   Middle entry clone

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-Merlin S518D

   Plasmid

   #84295

   Purpose

   Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Merlin (NF2) (NF2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S518D

   Promoter

   CMV

   Available Since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28b-RfxCas13d-His

   Plasmid

   #141322

   Purpose

   Plasmid for bacterial expression and purification of RfxCas13d protein

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40

   Plasmid

   #100831

   Purpose

   AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Eric Schreiter, Loren Looger

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHiUGE HA-epitope donor ORF0

   Plasmid

   #200371

   Purpose

   Tagging endogenously expressed proteins with HA epitope, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   HA epitope
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-PKCA D463N

   Plasmid

   #195203

   Purpose

   Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

   Depositor

   Peter Parker, David Bryant

   Article

   Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

   Insert

   PRKCA (PRKCA Human)
   

   Expression

   Mammalian

   Mutation

   D463N

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. gTBEv4

   Plasmid

   #220620

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
   

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-VEGFR2-V5/HIS

   Plasmid

   #205617

   Purpose

   Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR2 receptor tyrosine kinase (KDR Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-SEP-GluA2 TKIT

   Plasmid

   #169442

   Purpose

   Expression of 2 guides + donor DNA

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   AAV and CRISPR

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG_smFP FLAG

    Plasmid

    #59756

    Purpose

    encodes multiple copies of an epitope tag FLAG

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _FLAG
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG_smFP FLAG

    Plasmid

    #59756

    Purpose

    encodes multiple copies of an epitope tag FLAG

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _FLAG
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbAk-w4-loxP-TT-loxP-mCherry-PCB

    Plasmid

    #228469

    Purpose

    Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production

    Depositor

    Mary Dunlop

    Article

    Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

    Inserts

    w4-loxP-TT-loxP-mCherry
    Ho1
    PcyA
    

    Expression

    Bacterial

    Promoter

    J23106, J23108, and T7A1

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFBP-2_6.sensor

    Plasmid

    #162800

    Purpose

    Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    2_6_RNA-device
    

    Expression

    Yeast

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-tmpknot-GG-acceptor

    Plasmid

    #174039

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_DKK1_WT

    Plasmid

    #82250

    Purpose

    Gateway Donor vector containing DKK1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    DKK1 (DKK1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3962 pcDNA3-IRF3-FL

    Plasmid

    #22860

    Depositor

    Peter Howley

    Article

    Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

    Insert

    IRF3 (IRF3 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3962 pcDNA3-IRF3-FL

    Plasmid

    #22860

    Depositor

    Peter Howley

    Article

    Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

    Insert

    IRF3 (IRF3 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TAC-OSKM

    Plasmid

    #80481

    Purpose

    piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mRFP-Lact-C2

    Plasmid

    #74061

    Purpose

    Fluorescent biosensor for phosphatidylserine.

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 (MFGE8 Bovine)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. mRFP-Lact-C2

    Plasmid

    #74061

    Purpose

    Fluorescent biosensor for phosphatidylserine.

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 (MFGE8 Bovine)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. Dronpa3-C1

    Plasmid

    #54655

    Purpose

    Localization: C1 Cloning Vector, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    Dronpa3

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

22. Dronpa3-C1

    Plasmid

    #54655

    Purpose

    Localization: C1 Cloning Vector, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    Dronpa3

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pDONR221_SLC4A7

    Plasmid

    #132204

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC4A7 (SLC4A7 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pCAG-FIP200 (1-640)-msfGFP

    Plasmid

    #203546

    Purpose

    FIP200 NTD-GFP-10His mammalian expression

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Tags

    msfGFP-10His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Tol2-UAS-Dendra2

    Plasmid

    #97145

    Purpose

    For Gal4/UAS expression system

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    Dendra2
    

    Use

    Zebrafish

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.GFAP.Cre.WPRE.hGH

    Plasmid

    #105550

    Purpose

    AAV expression of Cre from GFAP promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB and AAV5

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    GFAP

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G

    Plasmid

    #194457

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-CD19 CAR
    

    Use

    Lentiviral

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EGFP-Cyclin F

    Plasmid

    #215054

    Purpose

    Expresses EGFP-Cyclin F in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCNF (CCNF Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV-CAG-SV40NLSf-mScarlet-P2A-hLuc-WPRE

    Plasmid

    #203539

    Purpose

    AAV reporter genome plasmid

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    NLS-mScarlet-2A-mScarlet
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSimpleII-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47867

    Purpose

    This plasmid encode NmCas9 with two NLS and a HA tag

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Insert

    NmCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSimpleII-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47867

    Purpose

    This plasmid encode NmCas9 with two NLS and a HA tag

    Depositor

    James Thomson, Erik Sontheimer

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Insert

    NmCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TFORF1761

    Plasmid

    #142843

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF24 (ZNF24 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCCL-c-MNDU3-X

    Plasmid

    #81071

    Purpose

    Empty lentiviral backbone with the MNDU3 promoter

    Depositor

    Donald Kohn

    Article

    Logan et al Hum Gene Ther. 2004 Oct;15(10):976-88.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MNDU3

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28-SaCas9

    Plasmid

    #178901

    Purpose

    SaCas9 (Cas9 from Staphylococcus aureus)

    Depositor

    Hendrik Milting

    Article

    Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

    Insert

    SaCas9
    

    Tags

    His6-Tag, bipartite nuclear localization signal f…

    Expression

    Bacterial

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EGFP-Actin-7

    Plasmid

    #56421

    Purpose

    Localization: Actin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. EGFP-Actin-7

    Plasmid

    #56421

    Purpose

    Localization: Actin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. GFP-ASH1L

    Plasmid

    #65369

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    ASH1L (ASH1L Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GFP-ASH1L

    Plasmid

    #65369

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    ASH1L (ASH1L Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171617

    Purpose

    AAV Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    AAV and Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pm-iATPSnFR1.0

    Plasmid

    #102548

    Purpose

    membrane displayed ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Expression

    Mammalian

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge